Characterization of Bacillus thuringiensis soil isolates from Cuba, with insecticidal activity against mosquitoes

Abstract

Chemical insecticides may be toxic and cause environmental degradation. Consequently, biological control for insects represents an alternative with low ecological impact. In this work, three soil isolates (A21, A51 and C17) from different regions of the Cuban archipelago were identified, characterized and evaluated against Aedes aegypti and Culex quinquefasciatus. The new isolates were compared with reference IPS82 strain and two strains isolated from biolarvicides Bactivec and Bactoculicida, respectively. The differentiation was done by morphological, biochemical, bioassays activity and molecular methods (SDS-PAGE, plasmid profile and random amplified polymorphic analysis). All isolates were identified as Bacillus thuringiensis. The A21, A51 and C17 isolates showed higher larvicide activity than Bactivec's isolated reference strain, against both A. aegypti and C. quinquefasciatus. A21 isolate had a protein profile similar to IPS82 and Bactivec strain. A51 and C17 isolates produced a characteristic proteins pattern. A21 and A51 isolates had plasmid patterns similar to IPS82 standard strain, while C17 isolate had different both plasmid profile and protein bands. All the studied isolates showed a diverse RAPD patterns and were different from the strains previously used in biological con

Isolation and characterization of entomopathogenic bacteria from soil samples from the western region of Cuba

“…There are reports of extensive isolation of the Bacillus genus in Cuba but these focused on the control of agricultural pests (Carreras 2009). In a previous short screening, three Cuban native strains with activity against Diptera of medical importance were isolated (González et al 2011). The present study was oriented to isolate enthomopathogenic bacteria against vectors of human illness also, but a higher number of samples from the western region of the Cuban archipelago were processed.…”

“…quinquefasciatus and Ae. aegypti than did the reference strain 266/2, which has been applied in Cuba for mosquito control (González et al 2011). In all these isolates a similar pattern protein was observed, with the major proteins of 135, 78, 66, and 27 kDa.…”

ISOLATION AND IDENTIFICATION OF NOVEL LOCAL ISOLATES OF Bacillus thuringiensis ACTIVE AGAINST RED PALM WEEVIL (RPW)

“…Bacillus thuringiensis soil isolates A21, A51 and C17 isolated from different regions of the Cuban archipelago showed higher larvicide activity than Bactivec's isolated reference strain, against both Aedes aegypti and Culex quinquefasciatus (González et al, 2011). Accordingly, This study describe the isolation and identification of novel isolates of B. thuringiensis, in order to study insecticidal activity of isolates produced proteins against Red Palm Weevil insect.…”

Biodegradation of carbofuran in soils within Nzoia River Basin, Kenya

“…Studies indicate that such physical characteristics, as observed in the isolates, are associated with gram positive rod-shaped bacteria. [37][38][39][40][41][42][43] …”