Characterization of Bacilli Isolated from the Gut of Rohu,Labeo rohita, Fingerlings and Its Significance in Digestion

Ghosh, Koushik; Sen, Sukanta; Ray, Arun Kumar

doi:10.1300/j028v12n03_04

Cited by 117 publications

(109 citation statements)

References 16 publications

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“…In Microbial management, native species isolated from aquatic digestive tract, have played important roles in aquaculture purposes. Similar research were done by Ghosh et al in 2002 with Bacillus circulans isolated from intestine of fish species, Labeo rohita, in diets supplemented with them [12,13]. Also, bacteria Lactobacillus fructivorans isolated from fish species, Sparus auratus, as well as Lactobacillus plantarum (L. plantarum) isolated from human feces, led to increased growth and survival of this fish, after supplementation with Artemia franciscana nauplii [14].…”

Section: Introductionsupporting

confidence: 74%

“…From above dilutions, a volume equal to 100 micro liters were taken and were transported to pellets containing culture medium of Bacillus cereus agar and triptic soy agar (TCA) and were distributed in it. The above pellets were incubated in incubator for 24 hours in temperature of 30C and forming colonies again were purely cultured in culture (TCA) medium [5,12,13].…”

Section: Mixtures Of Bacteriamentioning

confidence: 99%

“…Bacterial rates' increasing was determined by each ration equal to one single logarithmic unit. Similarly, also in Lactobacillus group, the experimental rations were prepared with these bacterial concentrations from their powder products and were mixed with other rations and their homogenation suspension was prepared [12,13,16].…”

Section: Supplementation Of Rationsmentioning

confidence: 99%

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Study on Bacillus Isolated from Intestine of Persian Sturgeon (Acipenser persicus) Comparing with Commercial Probiotics

Tabari

Karimzadegan

et al. 2016

ILNS

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Abstract. At present, probiotic bacteria are proposed as an important alternative for antibiotic elimination and for providing renewable products through biological control in cultivation systems of marine fish larvae. The aim of this study is to compare the effects of native and commercial probiotics on growth parameters of Persian sturgeon (Acipenser persicus) larvae fed with controlled biomar diet. Experimental diets with three probiotic bacterial mixture including commercial lactobacillus (L. plantarum and L. rhamnosus), commercial Bacillus (B. subtillis and B. licheniformis) and Bacillus isolated from intestine of Persian sturgeon (B. mycoides) were completed in two levels (6.1 and 7.2 log CFU in each g of feed) and were fed to larvae of Persian sturgeon in six experimental treatments. The control treatment was fed with diet without supplementation. The experiment was done in randomized design. In all the treatments, feed conversion index was increased considerably and had significant difference with control treatment and in all the experimental treatments influenced by probiotics were higher than that of control treatment (P<0.05). The highest rate means of special growth (1.84±0.21%) and thermal growth index (3.44±0.37%) was obtained respectively in treatment C1 and C2 (isolated from intestine). Maximum special growth rate (SGR %) were obtained from C1 (4.69±0.71%) and B2 (4.62±0.87%, commercial Bacillus). In addition, native probiotic bacteria had better performance than the non native commercial species as well as selection of suitable probiotic (natural) with respect to fish species, region and condition of culture plays a special importance role in enhancing the yield of Persian sturgeon larvae.

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Section: Introductionsupporting

confidence: 74%

Section: Mixtures Of Bacteriamentioning

confidence: 99%

Section: Supplementation Of Rationsmentioning

confidence: 99%

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Study on Bacillus Isolated from Intestine of Persian Sturgeon (Acipenser persicus) Comparing with Commercial Probiotics

Tabari

Karimzadegan

et al. 2016

ILNS

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“…Ghosh et al (2002) suggested from their in vitro studies on enzymeproducing bacterial flora that Bacillus circulans, B. pumilus, and B. cereus, isolated from the alimentary canal of Labeo rohita fingerlings were good producers of proteolytic enzymes, though they did not quantify the enzyme activity. Bairagi et al (2002), however, quantified the proteolytic activity in the bacterial strains isolated from nine freshwater teleosts.…”

Section: Discussionmentioning

confidence: 99%

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Mondal¹,

Roy²,

Sen³

et al. 2008

Acta Icth et Piscat

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Mondal S., Roy T., Sen S.K., Ray A.K. 2008. Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish. Acta Ichthyol. Piscat. 38 (1): 1-8.Background. The information on gut microflora in fish is scanty and there is a paucity of knowledge regarding microbial enzyme activity in fish gastrointestinal tracts. Although some information is available on the enzymeproducing bacteria in fish digestive tracts, almost nothing is known about their distribution in different regions of the gut. In the present study, an attempt has been made to investigate the distribution of enzyme-producing microflora in the foregut and hindgut regions of seven culturable freshwater teleosts. Materials and Methods. Isolation and enumeration of aerobic bacterial flora in the foregut and hindgut regions of the gastrointestinal tracts of seven freshwater teleosts of different feeding habits, namely rohu, Labeo rohita; catla, Catla catla; mrigal, Cirrhinus mrigala; bata, Labeo bata; orange-fin labeo, Labeo calbasu; Nile tilapia, Oreochromis niloticus; and climbing perch, Anabas testudineus, have been carried out. Microbial culture of the gut mucosa on selected nutrient media, following the enrichment culture technique, was done for bacterial isolation. Bacterial isolates were qualitatively screened on the basis of their extracellular enzyme-producing ability. The selected strains were further quantitatively assayed for amylase, cellulase and protease activities.Results. In general, bacterial population was lower in the foregut region of all the seven species of fish examined. Amylolytic strains were present in higher densities in the foregut region of orange-fin labeo and bata (12.20 × 10 3 CFU · g -1 gut tissue and 11.50 × 10 3 CFU · g -1 gut tissue, respectively) in comparison to the hindgut region. The cellulolytic population exhibited maximum densities in the hindgut region of bata (7.20 × 10 3 CFU · g -1 gut tissue) followed by the foregut region of the same fish (5.50 × 10 3 CFU · g -1 gut tissue). Amylolytic and cellulolytic bacterial flora was not detected in both the fore and hindgut regions of climbing perch. Proteolytic bacterial flora was found in all the species of fish studied and the maximum count was observed in the hindgut region of bata (13.40 × 10 3 CFU · g -1 gut tissue), orange-fin labeo (9.00 × 10 3 CFU · g -1 gut tissue), Nile tilapia (8.30 × 10 3 CFU · g -1 gut tissue) and climbing perch (7.20 × 10 3 CFU · g -1 gut tissue). Minimum count of proteolytic bacterial flora was observed in the foregut region of all the fishes studied. Peak amylase and cellulase activities were exhibited by bacterial strains isolated from the foregut of orange-fin labeo (266.43 ± 0.15 U) and the hindgut of bata (64.01 ± 0.42 U), respectively. Maximum protease activity was exhibited by a strain isolated from the hindgut region of orange-fin labeo (44.33 ± 0.09 U), followed by the strains isolated from the hindgut regions of climbing perch (32.87 ± 0.12 U), bata (29.71 ± 0.11 U), and Nile tilapia (29.46 ± 0.11 U). C...

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“…Colonies with different morphological appearance were isolated and streaked separately on TSA plates to check their purity. Isolated colonies were characterised and identified (Ghosh et al 2002). Among them Lr 1.1 strain of Bacillus circulans was selected for incorporation into diets for its excellent protease and moderate cellulase producing capacities.…”

Section: Isolation and Selection Of Intestinal Bacterial Fl Oramentioning

confidence: 99%

Growth and survival of rohu, Labeo rohita (Hamilton) spawn fed diets supplemented with fish intestinal microflora

Ghosh¹,

Sen²,

Ray³

2002

Acta Ichthyol. Piscat.

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A 21-day feeding experiment was conducted to assess the effects of diets supplemented with fish intestinal bacteria producing extracellular enzyme on growth and survival of rohu, Labeo rohita (Hamilton) spawn (average weight 0.3 mg). Intestinal bacteria were isolated from healthy rohu fingerlings s averag � w � ight 3.51 _ ±0.31 _ g). Six isocaloric (4.24 kcal·g-) and 1somtrogenous \approximately 35% crude protein) diets (Dl-D6) were prepared containing 32% fish meal, 34% mustard oil cake, 30% rice bran, 2o/o cod liver oil, and 2% vitamin premix. Experimental diets (D2-D6) were supplemented with the isolated bacterial strain Bacillus circu lans Lr 1

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Characterization of Bacilli Isolated from the Gut of Rohu,Labeo rohita, Fingerlings and Its Significance in Digestion

Cited by 117 publications

References 16 publications

Study on Bacillus Isolated from Intestine of Persian Sturgeon (<i>Acipenser persicus</i>) Comparing with Commercial Probiotics

Study on Bacillus Isolated from Intestine of Persian Sturgeon (<i>Acipenser persicus</i>) Comparing with Commercial Probiotics

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Growth and survival of rohu, Labeo rohita (Hamilton) spawn fed diets supplemented with fish intestinal microflora

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