Characterization of Avian γδ T-Cell Subsets after<i>Salmonella enterica</i>Serovar Typhimurium Infection of Chicks

Pieper, Jana; Methner, Ulrich; Berndt, Angela

doi:10.1128/iai.00788-10

Cited by 42 publications

(20 citation statements)

References 53 publications

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“…However, in caecal tonsils, we found evidence of infection induced activation of TCRc/d+CD8b+ cells, particularly by the increased expression of CD25 after each E. tenella infection. Increased mRNA expression of IL-2 receptor a and other immune genes in TCRc/d+CD8a/b+ cells has also been observed after Salmonella Typhimurium infection (47). Hence, these observations suggest that chicken TCRc/d+CD8b+ cells do have a role in infection responses and our results with similar responses upon repeated infections with the same pathogen indicate an innate function.…”

Section: Discussionsupporting

confidence: 80%

“…After the E. tenella infections, we did not observe any prominent alterations in the proportions of caecal or caecal tonsil TCRγ/δ+CD8β+ cells. Similarly, it has been reported that the proportions of TCRγ/δ+CD8α/β+ cells were not affected in caecum, blood and spleen at Salmonella enterica serovar Typhimurium infection or in blood at S. enterica serovar Enteritidis infection . Moreover, TCRγ/δ+CD8α/β+ cells did not increase cell surface CD25 expression upon Salmonella Enteritidis stimulation in vitro .…”

Section: Discussionmentioning

confidence: 54%

“…Without further differentiation of TCRγ/δ+ cells, these observations are difficult to correlate to the present study. Nonetheless, the chicken TCRγ/δ+CD8αα+ cell population, which was not included in the present analysis, has been shown to expand and/or be activated upon Salmonella infections in vivo and in vitro and could therefore also have been responsible for those earlier observed increases in TCRγ/δ+ cells after Eimeria infections.…”

Section: Discussionmentioning

confidence: 91%

“…Proposed functions for TCRc/d+CD8b+ cells and other TCRc/d+ cell subpopulations include, for example cytotoxcicity. Evidence in this matter is so far conflicting, results from experiments depleting different T-cell populations combined with in vitro CTL-assays point against TCRc/d+ cells having a role in cytotoxicity (51,52) while the expression of Fasligand mRNA in TCRc/d+CD8a/b+ cells suggest they may have cytolytic capacity (37,47). We found that TCRc/ d+CD8b+ cells readily responded with cell surface CD107a mobilization upon in vitro PMA-ionomycin stimulation, which supports that these cells may have cytolytic functions.…”

Section: Discussionmentioning

confidence: 99%

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Monitoring of local CD8β‐expressing cell populations during Eimeria tenella infection of naïve and immune chickens

Wattrang

Thebo

Lundén

et al. 2016

Parasite Immunology

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The purpose of this study was to monitor abundance and activation of local CD8β-expressing T-cell populations during Eimeria tenella infections of naïve chickens and chickens immune by previous infections. Chickens were infected with E. tenella up to three times. Caecal T-cell receptor (TCR) γ/δ-CD8β+ cells (cytotoxic T lymphocytes; CTL) and TCRγ/δ+CD8β+ cells were characterized with respect to activation markers (blast transformation, CD25 and cell surface CD107a). Cells were also induced to degranulate in vitro as a measure of activation potential. Major findings included a prominent long-lasting, up to 6 weeks, increase in the proportion of CTL among caecal CD45+ cells in the later stages after primary E. tenella infection. These CTL also showed clear signs of activation, that is blast transformation and increased in vitro induced degranulation. At second and third E. tenella infection, chickens showed strong protective immunity but discrete signs of cellular activation were observed, for example increased in vitro induced degranulation of CTL. Thus, primary E. tenella infection induced clear recruitment and activation of local CTL. Upon subsequent infections of strongly immune chickens cellular changes were less prominent, possibly due to lower overall numbers of cells being activated because of the severe restriction of parasite replication.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Monitoring of local CD8β‐expressing cell populations during Eimeria tenella infection of naïve and immune chickens

Wattrang

Thebo

Lundén

et al. 2016

Parasite Immunology

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“…The γδ lymphocyte population has a heterogenous structure and phenotype in the chicken, with association of subsets with particular tissues ( 55 ). In the caeca, the CD8 + αα + γδ population is thought to be the main activator of the adaptive response ( 56 ).…”

Section: The Adaptive Response To Infection and The Success Of Vaccinmentioning

confidence: 99%

Salmonella enterica in the Chicken: How it has Helped Our Understanding of Immunology in a Non-Biomedical Model Species

Wigley

2014

Front. Immunol.

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Salmonella infection of the chicken is important both as a source of foodborne human salmonellosis and as a source of disease in the chicken itself. Vaccination and other control strategies require an understanding of the immune response and as such have been important in understanding both mucosal immunity and more generally the response to bacterial infection. In this review, we discuss the contribution the study of avian salmonellosis has made to understanding innate immunity including the function of phagocytic cells, pattern recognition receptors, and defensins. The mucosal response to Salmonella infection and its regulation and the contribution this makes in protection against infection and persistence within the gut and future directions in better understanding the role of TH17 and Tregs in this response. Finally, we discuss the role of the immune system and its modulation in persistent infection and infection of the reproductive tract. We also outline key areas of research required to fully understand the interaction between the chicken immune system and Salmonella and how infection is maintained in the absence of substantive gastrointestinal disease.

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Immune Response in Mammals and Chickens

Morgan

2021

IgY-Technology: Production and Application of Egg Yolk Antibodies

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Characterization of Avian γδ T-Cell Subsets afterSalmonella entericaSerovar Typhimurium Infection of Chicks

Cited by 42 publications

References 53 publications

Monitoring of local CD8β‐expressing cell populations during Eimeria tenella infection of naïve and immune chickens

Monitoring of local CD8β‐expressing cell populations during Eimeria tenella infection of naïve and immune chickens

Salmonella enterica in the Chicken: How it has Helped Our Understanding of Immunology in a Non-Biomedical Model Species

Immune Response in Mammals and Chickens

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