Characterization of avian pathogenic Escherichia coli isolated from free-range helmeted guineafowl

Borzi, Mariana Monezi; Cardozo, Marita Vedovelli; Oliveira, Érika Arantes de; Pollo, Andressa de Souza; Guastalli, Elisabete Aparecida Lopes; Santos, Luis Fernando dos; Ávila, Fernando Antônio de

doi:10.1016/j.bjm.2018.04.011

Cited by 23 publications

(24 citation statements)

References 39 publications

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“…Low resistance frequency to sulphamethoxazole-trimethoprim was also reported in this study, contrasting with most data [59][60][61]66]. Among aminoglycosides, high resistance prevalence to streptomycin is widespread, but not to other drugs of this class [61][62][63]67]. Colistin and polymyxin B resistance were rarely described [59].…”

Section: E Colicontrasting

confidence: 81%

“…Regarding beta-lactams, ampicillin resistance frequency varies from around 20% [58] to over 80% [59], reaching 100% in clinical isolates [60,61]. Similar data have been described for first-generation cephalosporins [62,63], while low resistance has been reported to second and third-generation cephalosporins [64], except for ceftiofur, despite reports of low resistance frequencies in some collections [62,65]. Resistance to tetracycline is also reported with high frequencies, usually over 70%, but with exceptions again, including 13.3% in APEC strains [61].…”

Section: E Colisupporting

confidence: 68%

“…Vaz et al (2017) in Pernambuco State (NE) studied E. coli strains isolated from poultry liver carcasses and detected multidrug resistance with frequencies up to 48%, varying according to the farm [67]. Borzi et al (2017) studied E. coli recovered from free-range helmeted guinea fowl in São Paulo State (SE), and multidrug resistance was detected in 90.4% of the isolates [62]. Moreover, Maciel et al (2017) reported that two E. coli isolates recovered from an avian colisepticemia outbreak in the Rio Grande do Sul State (S) had resistance to all antimicrobial agents tested (ampicillin, tetracycline, gentamicin, neomycin, sulfa-trimethoprim, enrofloxacin, and norfloxacin) [60].…”

Section: E Colimentioning

confidence: 99%

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Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Rabello

Bonelli

Penna

et al. 2020

Animals

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In animal husbandry, antimicrobial agents have been administered as supplements to increase production over the last 60 years. Large-scale animal production has increased the importance of antibiotic management because it may favor the evolution of antimicrobial resistance and select resistant strains. Brazil is a significant producer and exporter of animal-derived food. Although Brazil is still preparing a national surveillance plan, several changes in legislation and timely programs have been implemented. Thus, Brazilian data on antimicrobial resistance in bacteria associated with animals come from official programs and the scientific community. This review aims to update and discuss the available Brazilian data on this topic, emphasizing legal aspects, incidence, and genetics of the resistance reported by studies published since 2009, focusing on farm animals and derived foods with the most global public health impact. Studies are related to poultry, cattle, and pigs, and mainly concentrate on non-typhoid Salmonella, Escherichia coli, and Staphylococcus aureus. We also describe legal aspects of antimicrobial use in this context; and the current occurrence of genetic elements associated with resistance to beta-lactams, colistin, and fluoroquinolones, among other antimicrobial agents. Data here presented may be useful to provide a better understanding of the Brazilian status on antimicrobial resistance related to farm animals and animal-derived food products.

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Section: E Colicontrasting

confidence: 81%

Section: E Colisupporting

confidence: 68%

Section: E Colimentioning

confidence: 99%

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Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Rabello

Bonelli

Penna

et al. 2020

Animals

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“…Bacterial suspension (5 µL) was streaked onto the plates, and the plates were incubated at 37 °C for 24 h. The strong biofilm-producing isolates were visualized as deep brick red-colored. For more confirmation, colonies were also consecutively examined following 48 and 72 h of incubation, and the results were interpreted as +++, ++ and + depending on their color intensity [ 23 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

“…The P fimbriae are important factors for the beginning and expansion of human urinary tract infections [ 30 ]; however, their role in the pathogenesis of avian colibacillosis is not yet clearly understood. The role of curli fimbriae which encodes for crl and csg A genes in the pathogenesis is poorly elucidated, though these genes facilitate the adherence of APEC strains to fibronectin and laminin [ 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

et al. 2020

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The avian pathogenic Escherichia coli (APEC) strains are the chief etiology of colibacillosis worldwide. The present study investigated the circulating phylotypes, existence of virulence genes (VGs), and antimicrobial resistance (AMR) in 392 APEC isolates, obtained from 130 samples belonged to six farms using both phenotypic and PCR-based molecular approaches. Congo red binding (CRB) assay confirmed 174 APEC isolates which were segregated into ten, nine, and eight distinct genotypes by RAPD assay (discriminatory index, DI = 0.8707), BOX-PCR (DI = 0.8591) and ERIC-PCR (DI = 0.8371), respectively. The combination of three phylogenetic markers (chuA, yjaA and DNA fragment TspE4.C2) classified APEC isolates into B23 (37.36%), A1 (33.91%), D2 (11.49%), B22 (9.20%), and B1 (8.05%) phylotypes. Majority of the APEC isolates (75–100%) harbored VGs (ial, fimH, crl, papC, and cjrC). These VGs (papC and cjrC) and phylotypes (D2 and B2) of APEC had significant (p = 0.004) association with colibacillosis. Phylogenetic analysis showed two distinct clades (clade A and clade B) of APEC, where clade A had 98–100% similarity with E. coli APEC O78 and E. coli EHEC strains, and clade B had closest relationship with E. coli O169:H41 strain. Interestingly, phylogroups B2 and D2 were found in the APEC strains of both clades, while the strains from phylogroups A1 and B1 were found in clade A only. In this study, 81.71% of the isolates were biofilm formers, and possessed plasmids of varying ranges (1.0 to 54 kb). In vitro antibiogram profiling revealed that 100% isolates were resistant to ≥3 antibiotics, of which 61.96%, 55.24%, 53.85%, 51.16% and 45.58% isolates in phylotypes B1, D2, B22, B23, and A1, respectively, were resistant to these antimicrobials. The resistance patterns varied among different phylotypes, notably in phylotype B22, showing the highest resistance to ampicillin (90.91%), nalidixic acid (90.11%), tetracycline (83.72%), and nitrofurantoin (65.12%). Correspondence analysis also showed significant correlation among phylotypes with CRB (p = 0.008), biofilm formation (p = 0.02), drug resistance (p = 0.03), and VGs (p = 0.06). This report demonstrated that B2 and A1 phylotypes are dominantly circulating APEC phylotypes in Bangladesh; however, B2 and D2 are strongly associated with the pathogenicity. A high prevalence of antibiotic-resistant APEC strains from different phylotypes suggest the use of organic antimicrobial compounds, and/or metals, and the rotational use of antibiotics in poultry farms in Bangladesh.

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Gamebird Skin Diseases and Multisystemic Diseases

Porter¹,

Morishita²

2022

Gamebird Medicine and Management

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Characterization of avian pathogenic Escherichia coli isolated from free-range helmeted guineafowl

Cited by 23 publications

References 39 publications

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

Gamebird Skin Diseases and Multisystemic Diseases

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