Characterization of Autonomic Nerve Markers and Lymphocyte Subsets in the Ileal Peyer’s Patch of Pigs Infected Experimentally with Brachyspira hyodysenteriae

Kaleczyc, Jerzy; Podlasz, Piotr; Winnicka, A.; Wąsowicz, Wojciech; Sienkiewicz, Waldemar; Żmudzki, J.; Łakomy, M

doi:10.1016/j.jcpa.2010.04.003

Cited by 20 publications

(24 citation statements)

References 31 publications

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“…550083, all from DB Pharmingen, USA) antibodies. Peyer's patch leukocytes for cytometry were obtained in accordance with the procedure described by Kaleczyc et al (2010). The samples were analyzed in a flow cytometer (FACScalibur, Becton Dickinson, USA), and the results were processed in the Cell QuestTM program (Becton Dickinson, USA).…”

Section: Lymphocyte Subpopulation Studymentioning

confidence: 99%

“…The ENS may undergo changes under the influence of various physiological and pathological stimuli such as aging, diet, inflammatory processes and other diseases (Gonkowski et al 2003, Vasina et al 2006, Gonkowski and Całka 2012. Lakomy et al (2009) and Kaleczyc et al (2010) demonstrated that cytokine expression in Peyer's patches and the correlations between the ENS and the immune system of the digestive tract can change during selected pathological processes.…”

Section: Introductionmentioning

confidence: 99%

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Zearalenone-induced changes in the lymphoid tissue and mucosal nerve fibers in the porcine ileum

Obremski¹,

Gonkowski²,

Wojtacha³

2015

Polish Journal of Veterinary Sciences

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This is the first study to examine zearalenone-(ZEN) induced changes in the immune system of the ileum and substance P-(SP-) and vasoactive intestinal peptide-(VIP-) immunoreactive nerve fibers in the mucosa, which participate in the regulation of intestinal functions under physiological conditions and during pathological processes. The aim of this study was also to identify potential relationships between selected immune and neural elements in ileal Peyer's patches in pigs that were and were not exposed to ZEN. The experiment was performed on 10 prepubertal gilts divided into two groups: the experimental group (n=5) where ZEN was administered at 0.1 mg kg -1 feed day -1 for 42 days, and the control group (n=5) which was administered a placebo. The tissue levels of cytokines were determined by enzyme-linked immunosorbent assay which revealed elevated concentrations of IL-12/23 40p and IL-1 β in animals exposed to ZEN. Flow cytometry revealed a lower percentage of CD21+ lymphocytes in pigs exposed to ZEN in comparison with control animals. The tissue levels of neuropeptides were evaluated in the dot blot procedure which demonstrated higher concentrations of VIP and SP in experimental pigs. In experimental animals, numerous VIP-like immunoreactive processes were observed, and SP-immunoreactive nerve fibers formed a very dense network. Our results demonstrate for the first time that ZEN can modify the chemical coding of nerve structures in the gastrointestinal system. Those modifications can be attributed to ZEN's impact on estrogen receptors or its pro-inflammatory properties, and they reflect changes that take place in the nervous system at the transcriptional, translational and metabolic level.

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Section: Lymphocyte Subpopulation Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zearalenone-induced changes in the lymphoid tissue and mucosal nerve fibers in the porcine ileum

Obremski¹,

Gonkowski²,

Wojtacha³

2015

Polish Journal of Veterinary Sciences

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“…Payer's patch leukocytes for cytometry were obtained in accordance with the procedure described by Kaleczyc et al (2010).…”

Section: Determination Of the Percentages Of T Lymphocyte Subpopulationsmentioning

confidence: 99%

The effect of in vivo exposure to zearalenone on cytokine secretion by Th1 and Th2 lymphocytes in porcine Peyer’s patches after in vitro stimulation with LPS

Obremski¹

2014

Polish Journal of Veterinary Sciences

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Most research studies investigating the estrogenic effects of zearalenone (ZEN) focus on the mycotoxin's effect on the reproductive system. Since estrogen receptors are present on various types of immunocompetent cells, ZEN can also modify diverse immune functions. This study analyzed immunocompetent cells isolated from Peyer's patches in the ileum of pigs administered ZEN in the estimated daily dose of 8 μg kg -1 BW (equivalent of 100 μg kg -1 feed per day -1 ). The objective of the study was to determine whether long-term exposure to low ZEN doses below the NOEL threshold leads to changes in the percentages of lymphocyte subpopulations and cytokine secretion by Th1

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“…As a result, they are often used as models to study a variety of disease mechanisms and different pathophysiological conditions in humans, such as obesity and gastrointestinal dysfunctions (Spurlock and Gabler 2008;Pidsudko et al 2011;Zhang et al 2013). Substance P was reported to be localised in the porcine central nervous system (Barnes et al 1993;Holm et al 1993) and in the nerve fibres innervating many porcine organs (Schmidt et al 1991;Happola et al 1993;Czaja et al 2001;Lamb and Sparrow 2002;Podlasz et al 2003;Pidsudko et al 2008;Podlasz and Wasowicz 2008;Kaleczyc et al 2010). In the pig, substance P can stimulate both endocrine and exocrine pancreatic functions through NK-1 receptors (Schmidt et al 2000).…”

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confidence: 99%

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Jakimiuk¹,

Podlasz²,

Chmielewska-Krzesińska³

et al. 2017

Vet. Med.

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Substance P is involved in many physiological and pathophysiological processes. This functional diversity is mediated by three neurokinin receptor subtypes (NK1R, NK2R and NK3R) encoded by the TACR1, TACR2 and TACR3 genes, respectively. Despite the increasing interest in using pigs (Sus scrofa) to study human disease mechanisms, the sequences of these receptors are still unconfirmed or in the case of the NK1 receptor, not yet even unpredicted. We employed in silico analysis to define the localisation of the porcine tachykinin receptor genes, and to predict the structures and amino acid sequences of the respective proteins. A reverse transcription polymerase chain reaction (RT-PCR) assay was performed to analyse the expression of tachykinin receptor genes in different porcine tissues. The data show that the TACR1 gene is located on chromosome 3, TACR2 on chromosome 14 and TACR3 on chromosome 8. All three genes encode proteins with structures that incorporate features of G-protein-coupled receptors with sizes of 407, 381 and 464 amino acids, respectively. The receptors display a high degree of similarity to other mammalian neurokinin receptors. The NK1R subtype is expressed in both the central nervous system and peripheral tissues, while NK2R expression seems to be localised mostly to peripheral tissues. The expression of NK3R is found mainly in the central nervous system. This report provides for the first time the results of a comprehensive analysis of the structure and distribution of porcine NK1R, as well as other porcine neurokinin receptors and their genes. We hope that our data may offer an invaluable foundation for the future studies on the function of diverse tachykinin peptides in the central nervous system and peripheral tissues.

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Characterization of Autonomic Nerve Markers and Lymphocyte Subsets in the Ileal Peyer’s Patch of Pigs Infected Experimentally with Brachyspira hyodysenteriae

Cited by 20 publications

References 31 publications

Zearalenone-induced changes in the lymphoid tissue and mucosal nerve fibers in the porcine ileum

Zearalenone-induced changes in the lymphoid tissue and mucosal nerve fibers in the porcine ileum

The effect of in vivo exposure to zearalenone on cytokine secretion by Th1 and Th2 lymphocytes in porcine Peyer’s patches after in vitro stimulation with LPS

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

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