Characterization of Austrian koi herpesvirus samples based on the ORF40 region

Marek, Ana; Schachner, Oskar; Bilić, Ivana; Heß, Michael

doi:10.3354/dao02170

Cited by 5 publications

(9 citation statements)

References 9 publications

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“…In this study, 6 of the KHV isolates were analyzed and shown to be identical to the Japanese type (GenBank accession number AP008984) based on an analysis of the KHV ORF40 region as suggested by Marek et al (2010). Therefore, the KHV detected in this study is likely derived from the Japanese strain.…”

Section: Discussionmentioning

confidence: 99%

“…Japan and Israel/USA, based on full-length sequence analysis. Bigarré et al (2009) (Marek et al 2010). …”

Section: Abstract: Carp · Cyprinus Carpio · Carassius Auratus · Cyprmentioning

confidence: 99%

“…The Indonesian lineage is more closely related to the Asian than the European lineage. The study of genetic relationships based on ORF40 demonstrated that Austrian isolates share 100% nucleotide sequence identity with the Japanese strain of KHV (Marek et al 2010). …”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the KHV detected in this study is likely derived from the Japanese strain. Similarly, Bigarré et al (2009) reported that most KHV recently isolated in the Netherlands were of the Japanese genotype, and Marek et al (2010) reported that 15 Austrian isolates were 100% identical to the Japanese strain. Marek et al (2010) suggested that an experimental infection using KHV isolates with various genetic characteristics is needed to analyze the effect of viral genetic type on viral pathogenicity and host mortality.…”

mentioning

confidence: 99%

“…Marek et al (2010), using primer sets targeting the ORF40 region, showed that KHV derived from Japan, USA, and Israel produced amplicons of 445, 458, and 460 bp, respectively. These primer sets were useful for the classification of KHV derived from various regions (Marek et al 2010).In this study, 6 of the KHV isolates were analyzed and shown to be identical to the Japanese type (GenBank accession number AP008984) based on an analysis of the KHV ORF40 region as suggested by Marek et al (2010). Therefore, the KHV detected in this study is likely derived from the Japanese strain.…”

mentioning

confidence: 99%

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Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

Cho¹,

Won²,

Kim³

et al. 2014

Dis. Aquat. Org.

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Koi herpesvirus (KHV) disease is a lethal disease in common carp, an important food fish in Asian countries, the seed of which is used in restocking programs for freshwater fishery management. We inspected apparently healthy seed stock of common carp Cyprinus carpio L. and Siberian crucian carp Carassius auratus for the presence of KHV using PCR-based diagnostic tests as a part of a stock enhancement program from 2009 to 2010 in Korea. Consequently, KHV was detected from 24 of 232 inspections with yearly detection percentages of 5.2% in 2009 and 15.5% in 2010 using PCR primer sets for TK or SphI-5 as recommended by the OIE Manual of Diagnostic Tests for Aquatic Animals. Results indicate that the SphI-5 primer set was slightly more sensitive than the TK primer set, as shown by a higher detection rate. To determine the genotype of the KHV strains detected in this study, ORF40-specific PCR amplification was conducted, and the PCR products from 6 samples showed 100% nucleotide sequence identity with a Japanese strain (GenBank accession number AP008984) but not with US (DG657948) and Israeli strains (DG177346). This report conclusively demonstrated the presence of KHV in externally healthy seed of common carp and Siberian crucian carp, indicating a possible risk that subclinically infected seed stock can be released with a potential threat to wild populations. KEY WORDS: Carp · Cyprinus carpio · Carassius auratus · Cyprinid herpesvirus 3 · PCR · ORF40Resale or republication not permitted without written consent of the publisher Dis Aquat Org 112: [29][30][31][32][33][34][35][36] 2014 Marine and inland stock enhancement by releasing aquatic animals raised in hatcheries has been considered a potential management tool for increasing production and profitability of fisheries and meeting the demand for food requirements for an ever-increasing human population (Bartley et al. 2006). In Korea, hatchery-reared carp seed, especially common carp and Siberian crucian carp, have been released into the environment for the purpose of restoring the indigenous fish species and replenishing severely depleted fisheries in inland ranching programs.Concerns about unfavorable consequences of hatchery-reared seed releases such as the spread of pathogens, competition for food, and cannibalism, as well as genetic deterioration of wild stocks, have been increasing within the framework of aquatic animal health management (Fushimi 2001). Subasinghe (2005) also pointed out that movements of live aquatic animals and animal products (broodstock, seed, and feed) have accelerated the accidental spread and incursion of diseases into new populations and geographic regions, for example, through movements of hatchery-produced stocks. Thus special efforts have been required to prevent disease spread due to the release of cultured seed into the ecosystem, in terms of aquatic animal health management (Blankenship & Leber 1995).To prevent transmission of infectious agents by releasing hatchery-raised seed into the wider environment as part of...

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Section: Discussionmentioning

confidence: 99%

“…Japan and Israel/USA, based on full-length sequence analysis. Bigarré et al (2009) (Marek et al 2010). …”

Section: Abstract: Carp · Cyprinus Carpio · Carassius Auratus · Cyprmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

Cho¹,

Won²,

Kim³

et al. 2014

Dis. Aquat. Org.

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Genetic Diversity of Cyprinid Herpesvirus 3 from Different Geographical Locations during 1999–2019 in the United States of America

et al. 2020

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Cyprinid herpesvirus 3, also known as koi herpesvirus (KHV), is an important pathogen in common and koi carp Cyprinus carpio, varieties. Two main genotypes of KHV have been reported worldwide that are associated with Asian and European origins. In the USA, outbreaks of KHV diseases have been reported in different states since the early 1990s; however, the diversity of KHV is unknown. In the current study, 67 DNA samples that were extracted from clinical cases of koi tissues that were submitted for diagnosis during KHV outbreaks from 10 different states in the USA from 1999 to 2019 were used to investigate their genetic diversity. The thymidine kinase gene was amplified, sequenced, and used for phylogenetic analysis. Our results showed that the KHV isolates that were collected from the different states were clustered in the two known KHV genogroups, where 31 isolates belonged to the Asian genotype branch and 36 to the European genotype branch. The spatiotemporal analysis demonstrated fluctuation of KHV genotypes in the USA, as the main KHV genotype that was detected in koi in the USA from 1999 to 2013 was the European genotype, whereas the Asian KHV genotype appeared to emerge in the USA in 2008, increasing in incidence until 2019. The current study provides evidence on the genetic diversity of KHV in the USA. Future studies that evaluate the virulence of these genetically diverse isolates is warranted to obtain a better understanding of the epidemiology of this re‐emerging pathogen. This may provide an improved awareness of the current status of KHV and help to control the disease in the koi population in the USA.

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Emergence of fatal European genotype CyHV-3/KHV in mainland China

Dong

Weng

et al. 2013

Veterinary Microbiology

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Characterization of Austrian koi herpesvirus samples based on the ORF40 region

Cited by 5 publications

References 9 publications

Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

Genetic Diversity of Cyprinid Herpesvirus 3 from Different Geographical Locations during 1999–2019 in the United States of America

Emergence of fatal European genotype CyHV-3/KHV in mainland China

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