Characterization of atrial-natriuretic-factor-receptor-coupled membrane guanylate cyclase from rat and mouse testes

Marala, Ravi B.; Sharma, Rameshwar K.

doi:10.1042/bj2510301

Cited by 29 publications

(13 citation statements)

References 28 publications

(36 reference statements)

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“…These Vol. 282 (6 x l05 c.p.m./tube; specific radioactivity 3000 Ci/mmol) in the presence of 4 mM-Mg2" at room temperature for 10 min. Membranes were filtered through GF/C filters and washed four times with ice-cold phosphate-buffered saline, pH 7.5.…”

Section: Resultsmentioning

confidence: 99%

“…Atrial natriuretic factor (ANF) is a member of a family of structurally related peptide hormones which is primarily released from atria in response to volume expansion and which regulates sodium excretion, water balance, blood pressure [1][2][3] and steroidogenesis [4][5][6][7]. One important second messenger of ANFmediated biological processes is cyclic GMP [8].…”

Section: Introductionmentioning

confidence: 99%

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A structural motif that defines the ATP-regulatory module of guanylate cyclase in atrial natriuretic factor signalling

Goraczniak

Duda

Sharma

1992

Biochemical Journal

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Atrial natriuretic factor (ANF)-dependent guanylate cyclase is a single-chain transmembrane-spanning protein, containing an ANF receptor and having catalytic activity. ANF binding to the receptor domain activates the catalytic domain, generating the second messenger cyclic GMP. Obligatory in this activation process is an intervening step regulated by ATP, but its mechanism is not known. Through a programme of site-directed and deletion mutagenesis/expression studies, we report herein the identity of a structural motif (Gly503-Arg-Gly-Ser-Asn-Tyr-Gly509) that binds ATP and amplifies the ANF-dependent cyclase activity; this, therefore, represents an ATP-regulatory module (ARM) of the enzyme, which plays a pivotal role in ANF signalling.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A structural motif that defines the ATP-regulatory module of guanylate cyclase in atrial natriuretic factor signalling

Goraczniak

Duda

Sharma

1992

Biochemical Journal

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“…Further studies, using the 125I-ANP radioreceptor methods, confirmed this hypothesis (9,10). Experimental results on cultured murine and rat Leydig cells demonstrated that ANP increases cyclic GMP levels, inducing a selective activation of particulate guanylate cyclase (6,11,12). Later, it has been observed that cGMP is able to stimulate testosterone production in murine and rat Leydig cells, suggesting that this nucleotide is involved as a second messenger in the steroidogenic effect of rat atriopeptin II (rAP-II) (13).…”

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confidence: 77%

The role of calcium ions in rat Leydig cell steroidogenesis induced by atrial natriuretic peptide

Mioni¹

1993

Acta Endocrinologica

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Foresta C, Mioni R. The role of calcium ions in rat Leydig cell steroidogenesis induced by atrial natriuretic peptide. Acta Endocrinol 1993:128:274-80. ISSN 0001-5598 The aim of this study was to clarify whether calcium ions play a role in the mechanisms by which rat atriopeptin II exerts its stimulatory effects on rat Leydig cell steroidogenesis. The absence of calcium in the medium and the calcium channel-blocker drug, verapamil, both significantly inhibited testosterone production in rat atriopeptin Il-treated rat Leydig cells. Similar effects were observed on human chorionic gonadotropin-stimulated Leydig cells. Furthermore, 8-Bromoguanosine 3\m=' \:5\m=' \cyclic mono\x=req-\ phosphate, the putative second messenger of atrial natriuretic peptide, significantly increased rat Leydig cell steroidogenesis, determining a stimulatory effect comparable to that obtained with rat atriopeptin II. Calcium-free medium and verapamil inhibited this stimulatory effect. The addition of a calcium ionophore, A-23187, significantly counteracted the inhibitory effect of verapamil on rat atriopeptin I I \ x=r eq-\ induced and 8-Bromoguanosine 3\ m=' \ : 5\ m=' \ monophosphate-induced testosterone production. Furthermore, rat atriopeptin II significantly stimulated the calcium flux through the plasma membrane, as evaluated by the 45Ca2+ uptake method. This effect was strongly inhibited by the addition of verapamil. These data underline that calcium ions may play a role in the mechanisms by which atriopeptin II stimulates rat Leydig steroidogenesis, although other post-receptor systems cannot be excluded.

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“…A potential role for ANF in the maturation of the oocyte was previously suggested by the demonstration of ANF receptors in mammalian gonads [3,4,19] and the ability of these receptors to mediate ANF-induced increases in steroid production. The peptide stimulates testosterone production in mouse interstitial cells [20] and enhances both basal and gonadotropin-stimulated testosterone secretion from mouse Leydig cells [21].…”

Section: Potentiation Of Oocyte Maturationmentioning

confidence: 94%

Atrial Natriuretic Factor Activates Cyclic Adenosine 3′,5′-Monophosphate Phosphodiesterase in Xenopus Laevis Oocytes and Potentiates Progesterone-Induced Maturation via Cyclic Guanosine 5′-Monophosphate Accumulation

Sandberg¹,

Bor²,

Ji³

et al. 1993

Biology of Reproduction

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Xenopus oocytes were found to express atrial natriuretic factor (ANF) receptors that activate guanylate cyclase and stimulate cyclic guanosine 5'-monophosphate (cGMP) production in a dose- and time-dependent manner. A truncated fragment of ANF, known to bind to mammalian ANF receptors without stimulating cGMP accumulation, did not elicit a cGMP response in oocytes. In addition, preincubation with ANF increased the number of oocytes that underwent progesterone-induced maturation. The maximally effective dose of ANF (1 microM) elevated intracellular and extracellular cGMP accumulation from 50 to 200 and 5 to 800 fmol/oocyte, respectively, and increased the number of maturing oocytes by up to 3-fold. ANF's effects on progesterone-induced maturation were mimicked by nonhydrolyzable analogues of cGMP. ANF and 8-Br-cGMP had no effect on maturation in the absence of progesterone, indicating that elevation of cGMP alone is not sufficient to induce maturation. Dibutyryl-cGMP was as effective as 8-Br-cGMP, whereas 8-Br-guanosine monophosphate, 8-Br-guanosine, and 8-Br-cyclic inosine monophosphate did not potentiate ovum maturation. In Xenopus oocytes, an initial step in progesterone-induced maturation is the reduction of intracellular cAMP levels; both ANF and 8-Br-cGMP lowered cAMP levels and enhanced progesterone's ability to do so. This decrease in cAMP levels was attributable to increased cAMP-phosphodiesterase activity, which was enhanced by both ANF and 8-Br-cGMP. These findings, and the presence of functional ANF receptors on Xenopus oocytes, demonstrate that ANF can participate in ovum development by stimulation of cGMP accumulation and activation of cAMP phosphodiesterase, thereby potentiating progesterone's ability to decrease cAMP levels and promote ovum maturation.

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Characterization of atrial-natriuretic-factor-receptor-coupled membrane guanylate cyclase from rat and mouse testes

Cited by 29 publications

References 28 publications

A structural motif that defines the ATP-regulatory module of guanylate cyclase in atrial natriuretic factor signalling

A structural motif that defines the ATP-regulatory module of guanylate cyclase in atrial natriuretic factor signalling

The role of calcium ions in rat Leydig cell steroidogenesis induced by atrial natriuretic peptide

Atrial Natriuretic Factor Activates Cyclic Adenosine 3′,5′-Monophosphate Phosphodiesterase in Xenopus Laevis Oocytes and Potentiates Progesterone-Induced Maturation via Cyclic Guanosine 5′-Monophosphate Accumulation

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