Characterization of aStaphylococcus aureusTransposon, Tn5405,Located within Tn5404and Carrying the Aminoglycoside Resistance Genes,aphA-3andaadE

Derbise, Anne; Kg, Dyke; N, el Solh

doi:10.1006/plas.1996.0020

Cited by 71 publications

(53 citation statements)

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“…In the streptococci, this 3-kbp region contains a homolog of an ABC transporter of unknown function and mefA, a gene that, unlike the erm gene, confers resistance to macrolides but not to streptogramins and lincosamides. A 2-kb region immediately downstream of the ermG gene in CTnGERM1 contained sequences that had been found previously on a Staphylococcus aureus ermB-containing plasmid (6). Other sequences in the downstream region included a homolog of a Bacteroides fragilis IS element (17).…”

Section: Vol 71 2005 Distribution Of the Ermg Gene 4931mentioning

confidence: 89%

Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents

Wang

Shoemaker

et al. 2005

Appl Environ Microbiol

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The ermG gene was first found in the soil bacterium Bacillus sphaericus. More recently, it was found in several human intestinal Bacteroides species. We report here the first finding of ermG genes in gram-positive bacteria isolated from porcine feces and from under-barn manure pits used to store porcine wastes. The porcine ermG sequences were identical to the sequence of the B. sphaericus ermG gene except that six of the seven ermGcontaining strains contained an insertion sequence element insertion in the C-terminal end of the gene. The porcine ermG genes were found in three different gram-positive genera, an indication that it is possible that the gene is being spread by horizontal gene transfer. A segment of a Bacteroides conjugative transposon that carries an ermG gene cross-hybridized with DNA from six of the seven porcine isolates, but the restriction patterns in the porcine strains were different from that of the Bacteroides conjugative transposon.

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Section: Vol 71 2005 Distribution Of the Ermg Gene 4931mentioning

confidence: 89%

Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents

Wang

Shoemaker

et al. 2005

Appl Environ Microbiol

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“…The DNA was digested with SmaI and subjected to pulsed-field gel electrophoresis as described previously (Fig. 1) (13). Concatameric bacteriophage lambda DNA molecules (48.5 kb; Bio-Rad) and the SmaI fragments of the cellular DNA from S. aureus NCTC 8325 were used as size standards.…”

Section: Methodsmentioning

confidence: 99%

Clonal Diversity among Streptogramin A-Resistant Staphylococcus aureus Isolates Collected in French Hospitals

Haroche¹,

Morvan²,

Davi³

et al. 2003

J Clin Microbiol

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We analyzed 62 clinical isolates of streptogramin A-resistant (SGA r ) Staphylococcus aureus collected between 1981 and 2001 in 14 hospitals located in seven French cities. These isolates, including five with decreased susceptibility to glycopeptides, were distributed into 45 antibiotypes and 38 SmaI genotypes. Each of these genotypes included between 1 and 11 isolates, the SmaI patterns of which differed by no more than three bands. Although numerous clones were identified, we observed the spread of monoclonal isolates either within the same hospital or within hospitals in distinct cities and at large time intervals. Hybridization with probes directed against 10 SGA r genes (vatA, vatB, vatC, vatD, vatE, vgaA, vgaB, vgaAv, vgbA, and vgbB) revealed six patterns: vgaAv (21 isolates), vatA-vgbA (24 isolates), vgaAv-vatB-vgaB (14 isolates), vgaAv-vatA-vgbA (1 isolate), vgaAv-vatA-vgbA-vatB-vgaB (1 isolate), and vgaA (1 isolate). We detected at least one SGA r determinant in all of the tested isolates. vgaAv, which is part of the recently characterized transposon Tn5406, was found in 59.7% of the tested isolates. Of the 16 streptogramin B-susceptible isolates, 14 carried vgaAv alone and were susceptible to the mixtures of streptogramins, whereas the 2 isolates carrying vgaAv-vatB-vgaB were resistant to these mixtures. vatA-vgbA was found on plasmids of the same apparent size in 26 (42%) of the tested clinical isolates from 18 unrelated SmaI genotypes. The possible dissemination of some of the multiple clones characterized in the present study with an expected increased selective pressure of streptogramins following the recent licensing of Synercid (quinupristin-dalfopristin) must be carefully monitored.Methicillin-resistant Staphylococcus aureus (MRSA) has become a major nosocomial pathogen worldwide. Glycopeptides have been the reference drugs for the treatment of MRSA infections (16,21,30). After the emergence of clones with decreased susceptibility to these antibiotics, first reported as sporadic cases in several countries and more recently also as outbreaks, alternative treatments such as quinupristin-dalfopristin (Synercid) have been promoted (17,21,33). Quinupristin and dalfopristin are derivatives of pristinamycins IA and IIA, respectively (10). Quinupristin-dalfopristin is an injectable, semisynthetic, mixture with a synergistic activity against most gram-positive pathogens. Since 1999, quinupristin-dalfopristin has been available for use in hospitals for the treatment of infections caused by gram-positive cocci that are resistant to other antibiotics.Quinupristin-dalfopristin and the natural antibiotics produced by streptomycetes, such as streptogramin, pristinamycin, synergistin, mikamycin, and virginiamycin, are mixtures of two classes of compounds, A and B, with distinct primary structures (10, 14). The A compounds are polyunsaturated cyclic macrolactones, and the B compounds are cyclic hexadepsipeptides. Both types of compounds bind different targets in the peptidyltransferase domain of the 23S ribosoma...

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“…The sat4 gene encoding streptothricin (nourseothricin) resistance has first been described in Campylobacter coli (Jacob et al, 1994;Bischoff and Jacob 1996). In staphylococci the aadE-sat4-aphA gene cluster is flanked by two copies of IS1182 constituting transposon Tn5405 (Derbise et al, 1996;Derbise et al, 1997). The aadE-sat4-aphA gene cluster is widespread among many Gram-positive genera and it remains to be speculative where this gene clusters originates from and subsequently spread to other bacteria.…”

Section: Aminoglycoside Resistancementioning

confidence: 99%

Current Trends of Emergence and Spread of Vancomycin-Resistant Enterococci

Werner¹

2012

Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium

182

229

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Characterization of aStaphylococcus aureusTransposon, Tn5405,Located within Tn5404and Carrying the Aminoglycoside Resistance Genes,aphA-3andaadE

Cited by 71 publications

References 0 publications

Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents

Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents

Clonal Diversity among Streptogramin A-Resistant Staphylococcus aureus Isolates Collected in French Hospitals

Current Trends of Emergence and Spread of Vancomycin-Resistant Enterococci

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