Characterization of Apatite from Human Teeth via XRD, FT-IR and TGA Techniques

Abstract

Human teeth (HT) are natural composites which consist of nanohydroxyapatite (HAp) arranged in lamellae and bound to collagen. In present study, prepared HT powders with different ages were characterized by X-Ray Diffraction (XRD), Fourier transform infrared (FT-IR) and thermogravimetric anlysis (TGA) techniques. HT at age ranges of 1-10, 11-20, 21-30, 31-40, 41-50 and 51-60 years old were selected for tests. To prepare samples, human teeth were washed in boiling water for 1 h and left in 1 M KOH solution for 6 h to remove any organic material. After HT samples were cleaned with ultrasonic in ethanol, they were then finely grounded using agate mortar. The results from XRD indicate that the major diffraction peaks of all samples with different ages were very closely identified to that of stoichiometric HAp. All HT powders were found to be nanocrystalline structure. Furthermore, it was found that the decrease in HAp crystallinity seem to increase with higher age of 31-40 years old. To investigate chemical structure confirmed by FT-IR, All HT powders showed the band positions and function groups, which are similar to that of HAp. group found in chemical structure indicates the structure of carbonated apatite. TGA results were found that the increase of weight loss seem to increase with higher age.

Calcium orthophosphates (CaPO4): occurrence and properties

“…However, data are available that the “central dark line” appears to be the calcium richest part of the human tooth enamel crystallites (Gasga et al 2008 ). As described above for bones, X-ray diffraction studies revealed that the biological apatite of younger teeth was less crystalline than that of more mature ones, while the maximum crystallinity was found for teeth belonged to people of 31–40 years old (Pankaew et al 2012 ). Therefore, maturation of teeth also means a slow transformation (re-crystallization?)…”

“…However, crystallinity of teeth belonged to older people (41–50 and 51–60 years old) was found to decrease with age. Simultaneously, thermogravimetric studies revealed bigger weight loss for teeth of the older people (Pankaew et al 2012 ), which could be due to a greater amount of carbonates.…”

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

“…However, data are available that the ''central dark line'' appears to be the calcium richest part of the human tooth enamel crystallites (Gasga et al 2008). As described above for bones, X-ray diffraction studies revealed that the biological apatite of younger teeth was less crystalline than that of more mature ones, while the maximum crystallinity was found for teeth belonged to people of 31-40 years old (Pankaew et al 2012). Therefore, maturation of teeth also means a slow transformation (re-crystallization?)…”

“…However, crystallinity of teeth belonged to older people (41-50 and 51-60 years old) was found to decrease with age. Simultaneously, thermogravimetric studies revealed bigger weight loss for teeth of the older people (Pankaew et al 2012), which could be due to a greater amount of carbonates.…”