Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD coupled with pre-column DPPH assay

Meda, Naamwin R.; Fraisse, Didier; Gnoula, Charlemagne; Vivier, Magali; Felgines, Catherine; Sénejoux, François

doi:10.1007/s00217-017-2873-7

Cited by 20 publications

(18 citation statements)

References 24 publications

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“…strong antioxidant activity among extracts from the five Moraceae species or hybrids, and 23 phenolic substances were identified via UPLC-QTOF-MS/MS. We next used DPPH/ABTS-guided HPLC assays, a rapid method for screening active antioxidant compounds from complex mixture extracts 36 , to screen effective antioxidants and evaluate the contribution of individual compounds to total antioxidant activity within a complex mixture extract 21 . When an antioxidant compound reacts with a radical such as DPPH• or ABTS• + , the result is a redox reaction; i.e., the molecular structure of the antioxidant changes 37 , with the peak area (PA) becoming significantly smaller in the chromatogram 38 .…”

Section: Screening Of Active Antioxidants By Dpph/abts-guided Hplc Anmentioning

confidence: 99%

“…Therefore, it is of great importance to develop a rapid and accurate technique to determine the specific ability of individual active compounds in the natural extracts of complexes. High-performance liquid chromatography (HPLC) analysis combined with DPPH and/or ABTS assays are currently implemented for rapid screening of active compounds in extracts 21,22 .…”

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confidence: 99%

“…In DPPH/ABTS-guided HPLC analysis, after a specific antioxidant in the extract reacts with DPPH• or ABTS• + , one or more hydrogen atoms from the former are transferred to the latter, leading to a decrease in, or extinction of, the antioxidant peak compared to that in the HPLC profile of the unreacted extract 21 . In total, 127 compounds have been identified from the so-called 'Yangxinshi Tablet' and 34 antioxidant compounds were rapidly screened using an online DPPH-guided HPLC assay 22 .…”

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confidence: 99%

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Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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Morus and Broussonetia trees are widely used as food and/or feed. Among 23 phenolics identified from leaves of five Moraceae species using UPLC-QTOF-MS/MS, 15 were screened using DPPH/ABTSguided HPLCs, including seven weak (flavonoids with one hydroxyl on B-ring) and eight strong (four caffeoylquinic acids and four flavonoids, each with a double hydroxyl on B-ring) antioxidants. We then determined the activity and synergistic effects of individual antioxidants and a mixture of the eight strongest antioxidants using DPPH-guided HPLC. Our findings revealed that (1) flavonoid glucuronide may have a more negative effect on antioxidant activity than glucoside, and (2) other compounds in the mixture may exert a negative synergistic effect on antioxidant activity of the four flavonoids with B-ring double hydroxyls but not the four caffeoylquinic acids. In conclusion, the eight phenolics with the strongest antioxidant ability reliably represented the bioactivity of the five extracts examined in this study. Moreover, the Morus alba hybrid had more phenolic biosynthesis machinery than its cross-parent M. alba, whereas the Broussonetia papyrifera hybrid had significantly less phenolic machinery than B. papyrifera. This difference is probably the main reason for livestock preference for the hybrid of B. papyrifera over B. papyrifera in feed.Morus and Broussonetia tree species (family: Moraceae) have high economic value; among other uses, their leaves are widely used as feed to improve meat quality. The genus Broussonetia comprises five species: B. papyrifera, B. kazinoki, B. zeylanica, B. kaempferi, and B. kurzii 1 , some of which are used as folk medicinal plants and/or for making bark paper 2 . The bark, fruits, leaves, and roots of B. papyrifera and B. kazinoki exhibit antioxidant, anti-inflammatory, antinociceptive, and anti-tyrosinase activity 3-6 . In China, B. papyrifera and B. kazinoki leaves, from which several bioactive components such as polyphenols, flavonoids and alkaloids have been isolated 7 , are widely used to feed livestock for meat quality improvement. Methanol extracts from B. papyrifera leaves have greater inhibitory effects on mushroom tyrosinase and 2,2-diphenyl-1-picrylhydrazyl/(DPPH)• scavenging activity 8 , and ethanol extracts from B. papyrifera roots inhibit both acetyl-and butyl-cholinesterases 2 . Papyriflavonol A, isolated from the root bark of B. papyrifera, exhibits strong antimicrobial activity against different pathogenic bacteria and fungi 9 , whereas a 70% ethanol leaf extract of B. kazinoki was reported to dramatically reduce ear and dorsal skin thickness and other clinical symptoms in mice with atopic dermatitis, possibly via the downregulation of immunoglobulin E (IgE) and interleukin-4 (IL-4) plasma levels 5 . Broussonone A extracted from B. kanzinoki stem bark displays noncompetitive inhibitory activity against pancreatic lipase 10 . However, data on the antioxidant activity and bioactive composition of B. kanzinoki remains limited.The genus Morus comprises 10-16 species 11 , of whic...

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Section: Screening Of Active Antioxidants By Dpph/abts-guided Hplc Anmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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“…On this basis, peak areas of radical scavenging compounds are reduced in HPLC chromatograms of spiked extracts whereas peak areas of inactive constituents are not affected. Of note, such a kind of approach has already been successfully adapted to identify DPPH [ 11 ] and ABTS [ 12 ], as well as peroxynitrite scavengers [ 13 ]. However, a method capable of detecting NO scavengers has yet to be devised.…”

Section: Introductionmentioning

confidence: 99%

A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Aloysia triphylla Leaves

et al. 2018

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The present study aimed at developing an original pre-column HPLC assay allowing rapid characterization of nitric oxide (NO) scavengers from complex plant extracts. Sodium nitroprusside (SNP) was employed as a NO donor and spiked with an aqueous extract from Aloysia triphylla leaves prior to HPLC analysis. Relying on the ability of radical scavenging constituents to be oxidized upon reaction with radicals, this assay successfully allowed direct identification of three potential NO scavengers, including verbascoside, isoverbascoside, and luteolin-7-O-diglucuronide. These three phenolics were also individually assessed for their NO scavenging activities by using a Griess colorimetric assay. With respective IC50 values of 56 ± 4, 51 ± 3, and 69 ± 5 µg/mL, verbascoside, isoverbascoside, and luteolin-7-O-diglucuronide were all reported as potent NO scavenging compounds, confirming the efficiency of the SNP spiking HPLC assay. The present method can, thus, be considered as a valuable and effective approach for speeding up the discovery of NO scavenging constituents.

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“…The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity was evaluated according to the method described by Meda et al [11], with slight modifications. Briefly, 10 µL of a solution of BRE (1 mg/mL in methanol) was mixed with 2.5 mL of fresh DPPH solution (25 µg/mL in methanol).…”

Section: Major Compounds Phenolic Content and Antioxidant Activitymentioning

confidence: 99%

In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium

et al. 2019

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Background: Some Bupleurum species, such as the Bupleurum chinense DC. or the Bupleurum scorzonerifolium Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of Bupleurum rotundifolium have been studied and showed anti-inflammatory or antiproliferative activities. This study was conducted to investigate the antioxidant, anti-inflammatory, and immunomodulatory effects of Bupleurum rotundifolium roots. Methods: To tackle the various aspects of inflammation, we studied in vitro a methanolic extract from the roots of Bupleurum rotundifolium on peripheral blood mononuclear cells (PBMCs), polymorphonuclear neutrophils (PMNs), and the monocytic cells THP-1. Its antioxidant capacities and iron-chelating activity were assessed. The extract was tested on THP-1 differentiation, reactive oxygen species (ROS) production by leukocytes, neutrophils chemotaxis, cytokines, PGE2 production, and NF-κB activation in PBMCs. Results: The extract showed a decreased ROS production in stimulated cells. It increased PBMC chemokine secretion and up-regulated the differentiation of THP-1 monocytes into macrophage-like cells, indicating a potential interest of the extract in the resolution of acute inflammation. In addition, the analysis of cytokine production suggests that Bupleurum rotundifolium has immunomodulatory properties. Conclusions: Cytokines secretion, especially IL-1β and IL-12p70, provided us with a set of indicators suggesting that the extract might be able to drive the polarization of macrophages and lymphocytes toward a Th2 anti-inflammatory profile in excessive inflammation. Formal analysis, J.C.; Funding acquisition, P.C.; Investigation, J.C.

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Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD coupled with pre-column DPPH assay

Abstract: Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD coupled with pre-column DPPH assa

Cited by 20 publications

References 24 publications

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Aloysia triphylla Leaves

In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium

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