1992
DOI: 10.1093/infdis/165.4.658
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Characterization of Antigenic Determinants of Borrelia burgdorferi Shared by Other Bacteria

Abstract: Three antigenic determinants of Borrelia burgdorferi that were shared with other spirochetes and gram-negative bacterial species, as measured by Western blot, ELISA, or both, were identified and characterized using monoclonal antibodies (MAbs). Two were associated with immunogenic integral membrane lipoproteins of 19- and 22-kDa, respectively, by [3H]palmitate incorporation and Triton X-114 phase partitioning. A third antigenic determinant was shown to be associated with a 72-kDa heat shock protein that was al… Show more

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Cited by 66 publications
(57 citation statements)
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“…Monoclonal antibodies kindly provided by other investigators included the following: anti-FlaB (H9724) by A. Barbour (University of California, Irvine, CA), anti-FlaA by B. Johnson (Centers for Disease Control and Prevention, Atlanta, GA), and anti-DnaK by J. Benach (SUNY, Stony Brook, NY). Specific reactivity to B. burgdorferi FlaA, FlaB, and DnaK has been reported previously (4,11,20,39,40).…”
Section: Methodssupporting
confidence: 68%
“…Monoclonal antibodies kindly provided by other investigators included the following: anti-FlaB (H9724) by A. Barbour (University of California, Irvine, CA), anti-FlaA by B. Johnson (Centers for Disease Control and Prevention, Atlanta, GA), and anti-DnaK by J. Benach (SUNY, Stony Brook, NY). Specific reactivity to B. burgdorferi FlaA, FlaB, and DnaK has been reported previously (4,11,20,39,40).…”
Section: Methodssupporting
confidence: 68%
“…The following antibodies were kindly provided by other investigators: Blair (University of Utah, Salt Lake City, UT). The specific reactivity of these antibodies to B. burgdorferi CheX, CheY3, Pfs, FlaB, MotB, FliG1, FliG2, and FliM has been reported previously (16,50,52,56,58,60,74,82).…”
Section: Methodssupporting
confidence: 53%
“…Monoclonal and polyclonal antibodies that were kindly provided by other investigators included the following: anti-FlaB (H9724) by A. G. Barbour (University of California, Irvine, CA), anti-FlaA by B. Johnson (Centers for Disease Control and Prevention, Atlanta, GA), anti-DnaK by J. Benach (SUNY, Stony Brook, NY), and polyclonal anti-CheA by P. Matsumura (University of Illinois, Chicago, IL). The specific reactivities of those antibodies to B. burgdorferi FlaB, FlaA, DnaK, and CheA2, respectively, have been reported previously (1,13,33,40,43). The intensity of the CheY3 protein bands in wild-type and complemented cheY3 ϩ was measured using Alpha View spot densitometry (Version 3.0.2.0; Alpha Innotech Corporations).…”
Section: Ry3-f (Ggatccattcaaaagactacaattgc) and Ry3-r (Aagcttt Ttaacamentioning
confidence: 95%