Characterization of Antigen-Presenting Cells in Fresh and Cultured Human Corneas Using Novel Dendritic Cell Markers

Mayer, Wolfgang J.; Irschick, Ulrike M; Moser, Patrizia; Wurm, Martin; Huemer, Hartwig P.; Romani, Nikolaus; Irschick, Eveline U.

doi:10.1167/iovs.06-1184

Cited by 94 publications

(95 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presumption that the dendritic cells observed with CCM are Langerhans cells and not another subset of dendritic cells is supported by previous findings that Langerhans cell‐specific surface markers are expressed by dendritic cells in the corneal and limbal epithelium 53, 54. Due to the fact that there are no animal studies for inflammatory neuropathies and that cell differentiation in the current study was based on morphological aspects, it is speculative to specify which immunological subtype was counted.…”

Section: Discussionsupporting

confidence: 64%

Corneal confocal microscopy in chronic inflammatory demyelinating polyneuropathy

Stettner

Hinrichs

Guthoff

et al. 2015

Ann Clin Transl Neurol

105

View full text Add to dashboard Cite

ObjectiveThere is an unmet need for better diagnostic tools to further delineate clinical subsets of heterogeneous chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) and multifocal motor neuropathy (MMN) to facilitate treatment decisions. Corneal confocal microscopy (CCM) is a noninvasive and reproducible nerve imaging technique. This study evaluates the potential of CCM as a diagnostic surrogate in CIDP and MMN.MethodsIn a cross‐sectional prospective approach, 182 patients and healthy controls were studied using CCM to quantify corneal nerve damage and immune cell infiltration.ResultsPatients with CIDP and MMN had a reduction in corneal nerve fiber (CNF) measures and an increase in corneal immune cell infiltrates. In CIDP, CNF parameters decreased with increasing duration of disease. The number of dendritic cells in proximity to CNFs was increased in patients with early disease and correlated with the degree of motor affection. A further reduction in CNF parameters and an increase in nondendritic cells were observed in patients with painful neuropathy. In CIDP patients with antineuronal antibodies the number of nondendritic cells was increased.InterpretationOur findings suggest that CNF loss may reflect severity of neuropathy and quantification of distinct cells around the CNF plexus may help in stratifying CIDP subtypes, clinical course, and disease activity. However, further longitudinal studies are required before CCM can be considered as a valid surrogate endpoint for patients with CIDP and MMN.

show abstract

Section: Discussionsupporting

confidence: 64%

Corneal confocal microscopy in chronic inflammatory demyelinating polyneuropathy

Stettner

Hinrichs

Guthoff

et al. 2015

Ann Clin Transl Neurol

105

View full text Add to dashboard Cite

show abstract

“…However, studies have also demonstrated that there are resident CD11b-positive cells present in the unwounded corneal stroma (Mayer et al, 2007). Therefore, although the authors believe it is likely most of the infiltrating cells detected in these experiments are bone marrow-derived cells, we cannot exclude the possibility that some cells are locally recruited CD11b-positive cells.…”

Section: Discussionmentioning

confidence: 69%

Topical interleukin-1 receptor antagonist inhibits inflammatory cell infiltration into the cornea

Stapleton

Chaurasia

Medeiros

et al. 2008

Experimental Eye Research

View full text Add to dashboard Cite

Interleukin (IL)-1α and β are important modulators of many functions of corneal epithelial and stromal cells that occur following injury to the cornea, including the influx of bone marrow-derived inflammatory cells into the stroma attracted by chemokines released from the stroma and epithelium. In this study, we examined the effect of topical soluble IL-1 receptor antagonist on bone marrowderived cell influx following corneal epithelial scrape injury in a mouse model. C57BL/6 mice underwent corneal epithelial scrape followed by application of IL-1 receptor antagonist (Amgen, Thousand Oaks, CA) at a concentration of 20 mg/ml or vehicle for 24 h prior to immunocytochemical detection of marker CD11b-positive cells into the stroma. In two experiments, topical IL-1 receptor antagonist had a marked effect in blocking cell influx. For example, in experiment 1, topical IL-1 receptor antagonist markedly reduced detectible CD11b-positive cells into the corneal stroma at 24 h after epithelial injury compared with the vehicle control (3.5 ± 0.5 (standard error of the mean) cells/400× field and 13.9 ± 1.2 cells/400× field, respectively, p < 0.01). A second experiment with a different observer performing cell counting had the same result. Thus, the data demonstrate conclusively that topical IL-1 receptor antagonist markedly down-regulates CD-11b-positive monocytic cell appearance in the corneal stroma. Topical IL-1 receptor antagonist could be an effective adjuvant for clinical treatment of corneal conditions in which unwanted inflammation has a role in the pathophysiology of the disorder.

show abstract

“…14 In our retrospective analysis, we found that even longer storage times of 421 days of organ culture led to a statistically significant decreased risk of immune reactions. From the in vitro results of the kinetics regarding the stromal APC decrease with the fastest depletion of stromal APCs during the first 7 days of organ culture 13 and our observation that the highest rate of rejections was in the group of grafts with storage times below 14 days compared with longer storage periods (see Figure 3) it might be concluded that stromal and not only epithelial DCs have an important role in corneal allograft rejection.…”

Section: Discussionmentioning

confidence: 73%

“…8 It has been shown that the number of LCs and DCs slowly decreases during organ culture, [9][10][11][12][13] although it seems that they do not vanish completely. Mayer et al 13 found that human corneas harbour different populations of LCs and DCs in a distribution pattern similar to that in the skin. In their kinetic study of DCs in organ culture, they found a significant decrease of APCs within the first 3 weeks of organ culture.…”

Section: Introductionmentioning

confidence: 99%

Prolonged organ culture reduces the incidence of endothelial immune reactions

Maier¹,

Heinzelmann²,

Böhringer³

et al. 2015

Eye

View full text Add to dashboard Cite

Purpose The number of antigen-presenting cells decreases during organ culture of corneoscleral discs. This might result in a decrease of immune reactions with increasing duration of organ culture. To investigate this hypothesis, we performed a retrospective analysis of all penetrating keratoplasties that were consecutively performed over the last 5 years. Patients and methods All cases of penetrating keratoplasties (n = 1006) were divided into two groups, with the division made at the median of the storage time (21 days). These two groups were compared by a Cox proportional hazards survival model regarding the incidence of endothelial immune reactions, clear graft survival, and chronic endothelial cell loss following penetrating keratoplasty considering patient's age, donor's age, and risk situation as co-variates. Results We observed statistically significantly fewer endothelial immune reactions (20.1% (95% confidence interval 15.5-24.5%) after 2 years) in the group with a storage time of more than 21 days compared with the group with a storage time of o21 days (26.5% (95% confidence interval 21.6-31.2%) after 2 years). However, the duration of organ culture did not have a statistically significant effect on clear graft survival or chronic endothelial cell loss. Conclusion Our results demonstrate that an increased duration of organ culture leads to a lower incidence of endothelial immune reactions following penetrating keratoplasty. However, we do not recommend increased storage times in general as overall graft survival did not improve. The reason for this apparent paradox may be that the endothelial cell count decreases during storage time.

show abstract

Characterization of Antigen-Presenting Cells in Fresh and Cultured Human Corneas Using Novel Dendritic Cell Markers

Cited by 94 publications

References 37 publications

Corneal confocal microscopy in chronic inflammatory demyelinating polyneuropathy

Corneal confocal microscopy in chronic inflammatory demyelinating polyneuropathy

Topical interleukin-1 receptor antagonist inhibits inflammatory cell infiltration into the cornea

Prolonged organ culture reduces the incidence of endothelial immune reactions

Contact Info

Product

Resources

About