Characterization of antibody-mediated inhibition of natural killer (NK) cytotoxicity: Evidence for blocking of both recognition and lethal hit stages of cytolysis

Kahle, Robert; Hiserodt, John C.; Bonavida, Benjamin

doi:10.1016/0008-8749(83)90097-7

Cited by 7 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In subsequent experiments using different batches of RAT* sera the blocking activity was similar to that seen with EGTA, i.e. restricted to the early stage of programming (Figure 3, Kahle et al 1983).…”

Section: Involvement Of Gp 95 Gp 140 Gp 180 In the Lethal Hit Smentioning

confidence: 55%

“…The results showed that RAT* IgG block NK targetconjugate formation. In addition, using the Ca^* pulse experiment, we saw that, similar to the effects seen with CTL (Kahle et al 1983), RAT* was capable of blocking the lethal hit event. Also noteworthy is our demonstration that RAT* also blocks cytotoxic factors derived from NK cells (Wright et al 1981), Taken together, these results suggest that RAT* recognizes membrane antigens or cell-free-extracts involved in the lethal hit event in both NK and CTL cytotoxic systems.…”

Section: Rat* Reveals That Ctl and Nk Cytotoxicity Share A Common Patmentioning

confidence: 69%

“…Considering that RAT* serum may be involved in blocking a step common to all cytotoxic systems, we investigated whether it could block natural killer cell (NK) cytotoxicity. It has been shown that NK-mediated lysis follows the same pathway as CTL, namely binding, programming for lysis and killer cellindependent target-cell lysis (Kahle et al 1983). We investigated the effect of RAT* on NK CMC.…”

Section: Rat* Reveals That Ctl and Nk Cytotoxicity Share A Common Patmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Interactions in T‐Cell‐Mediated Cytotoxicity

Bonavida

Bradley

Fan

et al. 1983

Immunological Reviews

Self Cite

View full text Add to dashboard Cite

Section: Involvement Of Gp 95 Gp 140 Gp 180 In the Lethal Hit Smentioning

confidence: 55%

Section: Rat* Reveals That Ctl and Nk Cytotoxicity Share A Common Patmentioning

confidence: 69%

Section: Rat* Reveals That Ctl and Nk Cytotoxicity Share A Common Patmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Interactions in T‐Cell‐Mediated Cytotoxicity

Bonavida

Bradley

Fan

et al. 1983

Immunological Reviews

Self Cite

View full text Add to dashboard Cite

“…The results suggest that these antibodies interfere with a post-binding step of the cytotoxic reaction. This distinguishes our mAb from other antibodies previously reported to inhibit NK by interfering with lymphocyte-target cell binding [22,25,56). On the other hand, Martz [16] and Neville and Hiserodt [17] have described antibodies that block cytotoxicity by human NK cells at a post-binding stage.…”

Section: Discussionmentioning

confidence: 78%

Monoclonal antibodies against leucoagglutinin‐reactive human T lymphocyte surface components: Two antibodies which inhibit cell‐mediated cytotoxicity at a post‐binding stage

Vargas‐Cortes¹,

Hammarström²,

Hammarström³

et al. 1986

Eur J Immunol

View full text Add to dashboard Cite

Two out of 20 monoclonal antibodies (IgM, kappa), mAb 3192 and mAb K3G, raised against leucoagglutinin-reactive components on human T cells, effectively blocked lymphocyte-mediated cytotoxicity in vitro. No antigenic polypeptide reactive with these antibodies has been identified thus far. However, they have previously been shown to react specifically with certain neutral glycolipids obtained from spleen. Both mAb inhibited the cytotoxicity of natural killer (NK) cells against K562 cells, antibody-dependent cellular cytotoxicity (ADCC) towards antibody-coated bovine erythrocytes and cytotoxic T lymphocyte activity against allogeneic target cells. In both NK and ADCC, preincubation of the lymphocytes with different antibody concentrations resulted in a dose-dependent reduction of cytotoxicity. In contrast, preincubation of the target cells had no effect indicating that the mAb inhibited cytotoxicity at the effector cell level. When studied at the single-cell level, the mAb did not alter the number of lymphocytes forming conjugates with K562 but significantly reduced the frequency of conjugates containing dead target cells. Addition of the mAb to preformed conjugates resulted in a dose-dependent reduction in the proportion of conjugates containing dead target cells. Furthermore, mAb 3192 did not reduce the number of lymphocytes forming rosettes with bovine erythrocytes, indicating that inhibition of ADCC was not due to blocking of the effector cell-target cell interaction mediated by the Fc receptor of the effector cells. Taken together, these results suggest that the mAb inhibited cytotoxicity by interfering with a post-binding step common for the different cytotoxicity systems.

show abstract

Monoclonal Antibodies (G1.4, D7.5) Induce Secretion of Lytic Factors From Natural Killer and T Lymphocytes

Stein‐Streilein

Guffee

1989

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Monoclonal antibodies were made by standard procedures using mononuclear cells isolated from hamster lung tissue to immunize the mice. The Moabs (G1.4, D7.5), secreted by the selected hybridomas, were isotyped as IgG2a, Kappa and were lytic in the presence of complement for 20% lymphocytes from lymph nodes or lung tissue; 40-50% of lymphocytes from spleen or bone marrow; and 70-80% of thymocytes or nylon wool nonadherent lung or lymph node lymphocytes. Functional studies showed that prior incubation of either Moab with enriched natural killer (NK) and T lymphocyte subpopulations did not interfere with effector:target conjugate formation but did block the ability of the effector cells to lyse NK-sensitive targets. We conclude that the Moabs bind to a molecule(s) on NK and T lymphocytes. Further, the preincubation medium acquired the capacity to lyse preferentially NK-sensitive targets but not NK-insensitive targets. Interestingly, the Moabs were able to induce secretion of lytic factors from thymocytes and to induce resting peripheral T lymphocytes to acquire NK activity and release lytic factors. The ability of the Moab to signal release of lytic factors was retained even when monovalent Fab fragments were used; therefore, the Moabs initiate secretion by mechanisms that do not apparently involve crosslinking or Fc receptor interaction. G1.4 and D7.5 Moabs were functionally and antigenically crossreactive with human, rat, and hamster lymphocytes but not with mouse lymphocytes. Biochemical and immunochemical analysis showed that the G1.4 and D7.5 antigen on thymus cells and lung mononuclear cells has an apparent molecular weight of 27 kD. Although it is unlikely that there is an universal mechanism for secretion used by cells in general, it is interesting that Moab treatment of three different subpopulation of lymphocytes induced secretion of lytic factors and suggests that under physiologic conditions a common molecule might participate in induction of the secretion pathway. These studies lead to the hypotheses that 1) NK and T lymphocytes share a cell surface molecule that participates in signals that initiate secretion and that 2) the expression of the G1.4, D7.5 antigens precedes thymic differentiation.

show abstract

Characterization of antibody-mediated inhibition of natural killer (NK) cytotoxicity: Evidence for blocking of both recognition and lethal hit stages of cytolysis

Cited by 7 publications

References 17 publications

Molecular Interactions in T‐Cell‐Mediated Cytotoxicity

Molecular Interactions in T‐Cell‐Mediated Cytotoxicity

Monoclonal antibodies against leucoagglutinin‐reactive human T lymphocyte surface components: Two antibodies which inhibit cell‐mediated cytotoxicity at a post‐binding stage

Monoclonal Antibodies (G1.4, D7.5) Induce Secretion of Lytic Factors From Natural Killer and T Lymphocytes

Contact Info

Product

Resources

About