Characterization of Antibodies to Androgen-Dependent Secretory Proteins of the Mouse Dorsolateral Prostate*

Abstract

The mouse prostate is an attractive model for studying the relationship between epithelial-mesenchymal interactions and the mechanism of androgen action because of the volume of information on tissue interactions in the development of the prostate of this species and the existence of a mutant mouse lacking functional androgen receptors (Tfm mouse). In this paper the major proteins of the mouse dorsolateral prostate (DLP) have been described, and antibodies to these proteins have been characterized. The two most abundant secreted proteins were of 110,000-115,000 (Mj1) and 55,000-62,000 (Mj2) mol wt. They were glycosylated, androgen dependent, and appeared to exist in an oligomeric complex. Antibodies raised against mouse DLP secretion reacted mainly with Mj1, Mj2, and a minor protein of 140,000 mol wt (Mn1). The antibodies were of a high titer and recognized these three mouse DLP proteins by Western blotting, immunoprecipitation, and immunocytochemical techniques. Mj1 and Mj2 were antigenically similar to proteins in the mouse coagulating gland and in the rat DLP, but were not found in other organs. Immunocytochemical staining of the DLP from intact mice revealed many ducts that were lined by a tall columnar epithelium whose cells stained intensely. However, ducts that were distended with luminal secretion had a low columnar epithelium that rarely showed intracellular staining. These marker proteins and the antibodies to them will be useful for detecting androgen-dependent functional activity in tissue recombinant studies with a variety of experimental tissues.

Effect of COX-2 (PGE2) and IL-6 on Prostate Cancer Bone Metastases

“…Androgens regulate normal prostate development and are required for prostate tumorigenesis. In both development and carcinogenesis, many of the androgenic effects on prostate epithelial cells are elicited via signaling through stromal AR (38)(39)(40). We confirmed that PCa growth in the bone is stimulated by androgenic effects on stromal cells and specifically by activation of Wnt signaling in preosteoblasts.…”

Pathologic progression of autochthonous prostate cancer in the TRAMP model

“…In fact, reliable histologic comparisons between lobes of the prostate can only be determined through meticulous anatomical dissections at the time of necropsy or through utilization of additional immunohistochemical staining for proteins unique to particular lobes of the prostate. 18 The prostate glands can be readily distinguished histologically from the seminal vesicles that characteristically display a more prominent ®bromuscular stroma with granular lumenal secretions ( Figure 1G) and from the ampullary glands that display a more prominent ®bro-muscular stroma with`Swiss cheese' like secretions ( Figure 1H). …”

Identification of prostatic-secreted proteins in mice by mass spectrometric analysis and evaluation of lobe-specific and androgen-dependent mRNA expression

“…An early study revealed that spermine-binding protein (SBP) and serine protease inhibitor Kazal type-3 (SPI-KT3) are abundant in the mouse VP (Mills et al 1987a(Mills et al , 1987b. Proteins secreted from the dorso-lateral prostate (DLP) and AP have not yet been identified, although Cunha's group developed a specific polyclonal antibody for major DLP protein(s) to be used as a differentiation marker (Donjacour et al 1990).…”