Characterization of Antibodies Generated Against a Conserved Portion of Oviductal Glycoprotein (OGP) and Endogenous Hamster OGP and Their Ability to Decrease Sperm Binding to the Zona Pellucida In Vitro

Schmidt, Annette; Pa, Mavrogianis; Mb, O'Day-Bowman; Rc, Jaffe; Hg, Verhage

doi:10.1111/j.1600-0897.1997.tb00316.x

Cited by 15 publications

(13 citation statements)

References 36 publications

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“…Because pOSP was incubated with oocytes and sperm, modifications of zona pellucida receptors by co-operative action between pOSP and corticol granule contents may have occurred, as may alterations to the spermatozoa membrane. Results from this study appear to contradict findings observed in the hamster [49,50] and in humans [51]. Those studies [49][50][51] found an increased number of spermatozoa bound to the oocyte when exposed to OSP.…”

Section: Discussioncontrasting

confidence: 82%

“…Results from this study appear to contradict findings observed in the hamster [49,50] and in humans [51]. Those studies [49][50][51] found an increased number of spermatozoa bound to the oocyte when exposed to OSP. The differences between our data and these previous findings may result from species-specific differences, purity of OSP, and use of oocytes matured and fertilized in vitro during our study.…”

Section: Discussioncontrasting

confidence: 82%

“…Interpreting many of these studies becomes complicated, because the purity of OSP preparations has varied. In this study, as well as those of Schmidt et al [49,50] and Martus et al [47], antibodies specific to that particular species OSP were used to eliminate the possibility of observed effects resulting from copurified or contaminant proteins. To our knowledge, however, no such experiments have been done for other reported studies.…”

Section: Discussionmentioning

confidence: 99%

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Effects of the Porcine Oviduct-Specific Glycoprotein on Fertilization, Polyspermy, and Embryonic Development In Vitro1

Kouba

Abeydeera

Alvarez

et al. 2000

Biology of Reproduction

125

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This study evaluated the effects of porcine oviduct-specific glycoprotein (pOSP) on in vitro fertilization (IVF), polyspermy, and development to blastocyst. Experiment 1 evaluated the effects of various concentrations (0-100 microgram/ml) of purified pOSP on fertilization parameters, including penetration, polyspermy, male pronuclear formation, and mean number of sperm penetrated per oocyte. Experiment 2 examined the ability of an anti-pOSP immunoglobulin G to inhibit the observed effects of pOSP on fertilization parameters. Experiments 3 and 4 examined various concentrations of pOSP (0-100 microgram/ml) on zona pellucida solubility and sperm binding, respectively. Lastly, experiment 5 assessed the effects of various concentrations of pOSP (0-100 microgram/ml) on the in vitro embryo cleavage rate and development to blastocyst. Pig oocytes matured and fertilized in vitro were used for all experiments. An effect of treatment (P < 0.05) was detected for pOSP on penetration, polyspermy, and mean number of sperm per oocyte. Concentrations for pOSP of 0-50 microgram/ml had no effect on sperm penetration rates; however, compared with the control, 100 microgram/ml significantly decreased the penetration rate (74% vs. 41%). Addition of 10-100 microgram/ml significantly reduced the polyspermy rate compared with the control (61% vs. 24-29%). The decrease in polyspermy achieved by addition of pOSP during preincubation and IVF was blocked with a specific antibody to pOSP. No effect of treatment was observed on zona digestion time relative to the control; however, the number of sperm bound to the zona pellucida was significantly decreased by treatment (P < 0.05). Compared with the control, all concentrations of pOSP examined reduced the number of sperm bound per oocyte (45 vs. 19-34). A treatment effect (P < 0.05) was observed for pOSP on embryo development to blastocyst but not on cleavage rates. Addition of pOSP during preincubation and fertilization significantly increased postcleavage development to blastocyst, but a synergistic stimulation on development was not detected when pOSP was included during in vitro culture. These results indicate that exposure to pOSP before and during fertilization reduces the incidence of polyspermy in pig oocytes, reduces the number of bound sperm, and increases postcleavage development to blastocyst.

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Section: Discussioncontrasting

confidence: 82%

Section: Discussioncontrasting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Effects of the Porcine Oviduct-Specific Glycoprotein on Fertilization, Polyspermy, and Embryonic Development In Vitro1

Kouba

Abeydeera

Alvarez

et al. 2000

Biology of Reproduction

125

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show abstract

“…It is a highly glycosylated molecule normally produced by the secretory epithelial cells of the oviduct (1,2). Its synthesis and secretion is regulated during the menstrual cycle with the highest concentrations of oviduct-specific glycoprotein found during the periovulatory period when estrogen levels are high and it is believed to play a role in fertilization and early embryonic development (3)(4)(5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Gain of OGP, an Estrogen-Regulated Oviduct-Specific Glycoprotein, Is Associated with the Development of Endometrial Hyperplasia and Endometrial Cancer

Woo

Alkushi

Verhage

et al. 2004

Clinical Cancer Research

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Purpose: Lesions in the endometrium are difficult to differentially diagnose. The present study examined whether oviduct-specific glycoprotein is differentially expressed in normal, hyperplastic, and malignant endometrium.Experimental Design: The expression of oviduct-specific glycoprotein was characterized by immunohistochemical methods with whole sections of endometrium from 90 women. An endometrial cancer tissue microarray with 200 cases of endometrial cancer was also assessed for oviductspecific glycoprotein, estrogen receptor, and PTEN expression.Results: In normal endometrium, there was focal oviduct-specific glycoprotein expression in the basalis layer, where the stem cells reside, in 10 of 15 cases. On average, atypical hyperplastic endometria stained more intensely than hyperplastic endometria (P ‫؍‬ 0.017), whereas the percentage of positively stained cells was not significantly different. The mean staining indices (intensity ؋ percentage of positive cells score) for hyperplasia and atypical hyperplastic were 4.7 and 5.5 and were significantly higher than staining indices seen in normal cycling endometria or welldifferentiated endometrioid endometrial carcinomas (P < 0.0001 and P < 0.001, respectively). The endometrial cancer tissue microarray showed that of 139 endometrioid endometrial carcinomas, 11 cases were strongly oviduct-specific glycoprotein positive, whereas the other 128 cases were negative or weakly positive. Analysis of Kaplan-Meier curves with log-rank statistics showed a trend toward significance, with strong oviduct-specific glycoprotein staining serving as a predictor of good prognosis (P ‫؍‬ 0.1). There was a significant positive correlation between oviduct-specific glycoprotein staining and loss of PTEN in the cases of endometrial cancer (P ‫؍‬ 0.004).Conclusions: Oviduct-specific glycoprotein may be a useful diagnostic adjunct to more accurately classify premalignant and early malignant change in the endometrium, improving on the current irreproducible histologic classification system.

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“…ZP3 is the primary speciesspecific binding site for sperm, and species-specifically induces sperm to undergo the acrosome reaction (21). ZP2 binds acrosome-reacted sperm and has been termed the secondary sperm receptor (22). The role of OGP in fertilization remains unclear, but estrous oviductal fluid, which would contain OGP, plays a role in species-specific gamete interaction (28).…”

mentioning

confidence: 99%

Positive Darwinian Selection Drives the Evolution of Several Female Reproductive Proteins in Mammals

Swanson

Yang²,

Wolfner³

et al. 2001

Obstetric and Gynecologic Survey

133

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Rapid evolution driven by positive Darwinian selection is a recurrent theme in male reproductive protein evolution. In contrast, positive selection has never been demonstrated for female reproductive proteins. Here, we perform phylogeny-based tests on three female mammalian fertilization proteins and demonstrate positive selection promoting their divergence. Two of these female fertilization proteins, the zona pellucida glycoproteins ZP2 and ZP3, are part of the mammalian egg coat. Several sites identified in ZP3 as likely to be under positive selection are located in a region previously demonstrated to be involved in species-specific spermegg interaction, suggesting the selective pressure is related to male-female interaction. The results provide long-sought evidence for two evolutionary hypotheses: sperm competition and sexual conflict.zona pellucida ͉ ZP3 ͉ fertilization ͉ sperm competition ͉ sexual conflict

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Characterization of Antibodies Generated Against a Conserved Portion of Oviductal Glycoprotein (OGP) and Endogenous Hamster OGP and Their Ability to Decrease Sperm Binding to the Zona Pellucida In Vitro

Cited by 15 publications

References 36 publications

Effects of the Porcine Oviduct-Specific Glycoprotein on Fertilization, Polyspermy, and Embryonic Development In Vitro1

Effects of the Porcine Oviduct-Specific Glycoprotein on Fertilization, Polyspermy, and Embryonic Development In Vitro1

Gain of OGP, an Estrogen-Regulated Oviduct-Specific Glycoprotein, Is Associated with the Development of Endometrial Hyperplasia and Endometrial Cancer

Positive Darwinian Selection Drives the Evolution of Several Female Reproductive Proteins in Mammals

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