Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia

Abstract

Bacterial resistance to antibiotics is one of the largest medical concerns worldwide.One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic resistant bacterial isolates from confirmed cases of urinary tract infections from an array of patients in Nariño, southern Colombia. Macro and microscopic descriptions of the 32 clinical isolates were conducted. Resistance profiles, biochemical, and molecular characterization (via 16S rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed. All the isolates were identified as E. coli and had resistance to beta-lactams, aminoglycosides, and fluoroquinolones. This resistance was related to plasmids carryingthebla TEM, blaSHV1, and blaCTXM1genes.There were significant differences between the resistance proportions of the samples (p value:0.0000), mainly to penicillin, cefotoxin, and imipenem. Using ERIC-PCR, four clonal states were evidenced that corroborate a degree of genetic differentiation with in the isolate set.Antibiotic resistance observed in the isolates is associated with resistance genes present in the bacterial chromosome and plasmids.

Bacterial Resistance in Hospital-Acquired Infections Acquired in the Intensive Care Unit: A Systematic Review

“…However, a study conducted in Mozambique using ERIC-PCR analysis revealed that despite evidence of high genetic diversity among E. coli isolates, there was a predominance of few clones adapted to the hospital environment, what would they probably be HiRCs (17). Similar findings were reported in hospitals in Ethiopia (25) and Colombia (64). Analysis by pulsed field gel electrophoresis (PFGE, technique used to produce a DNA fingerprint for a bacterial isolate) also supports these findings: among the great diversity of pulse types (ST), ST405 and ST1284 circulate mainly in hospitals in Lebanon (38), while ST131 in Bangladesh (35) and USA (89,100).…”

“…No most of them presented often resistance to fluoroquinolones, ampicillin, co-trimoxazole and cephalosporins (3,4,11,17,21,26,33,39,54,62,64,73,85,110). Moolchandani et al, recommends not using these antibiotics for empirical therapy of urinary tract infections acquired in ICUs in South India; instead, they suggest considering imipenem, pipericillin-tazobactam, amikacin, and nitrofurantoin for initial therapy with prompt de-escalation after culture and sensitivity results are received (3).…”

“…Isolates with Tn2006 has been detected in predominantly in Iran (113), while Tn2008, and Tn2009 in China (90,106). Also, high frequency of MDR pathogens harboring class 1 and 2 integrons have been detected in K. pneumoniae (9,14), A. baumannii (10,65,57,77,90,113), P. aeruginosa (18,94,101), and E. coli (38,64,110).…”

Antimicrobial Resistance Profiles and Co-Existence of Multiple Antimicrobial Resistance Genes in mcr-Harbouring Colistin-Resistant Enterobacteriaceae Isolates Recovered from Poultry and Poultry Meats in Malaysia

“…The genomic DNA of colistin-resistant isolates was subjected to PCR for the detection of antimicrobial-resistant genes against specific antibiotics, including β-lactamase genes ( bla CTX-M , bla TEM , and bla SHV ), Aminoglycosides (Gentamycin, aac-3-IV , Streptomycin, aadA1 ), Fluoroquinolones (Ciprofloxacin, aac ( 6- ) -lb ), Tetracyclines (Tetracycline, tetA ), Chloramphenicol ( catA1 and floR ) and Fosfomycins (Fosfomycin, fosA ) by previously designed oligonucleotide primers and protocols ( Tables S1 and S2 ) [ 36 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ].…”

Actividad antibacteriana de actinomicetos frente a Escherichia coli y Staphylococcus aureus multirresistentes de origen hospitalario

“…Se determinó mediante la técnica de Bauer Kirby el perfil de resistencia de las bacterias patógenas E. coli y S. aureus aisladas de muestras hospitalarias y conservadas en el Banco de Células Primarias del Laboratorio de Procesos Microbianos de la Universidad de Nariño se determinó mediante la técnica de Bauer Kirby (19,20) . Los antibióticos usados fueron: (P) penicilina, (SAM) ampicilina (10g), (AMC) amoxicilina (30g), (CTX) cefotoxima (30g), (STX) trimetroprim-sulfametoxazol (25g), (CN) gentamicina (10g), (ATM) aztreonam, (CIP) ciprofloxacina (5g), (IPM) imipenem, (AK) amikacina (30g), (S) estreptomicina (10g) y (EM) eritromicina (30g) (OXOID).…”