Characterization of antibiotic resistance determinants in oral biofilms

Kim, Seon-Mi; Kim, Hyeong C.; Lee, Seok-Woo S.

doi:10.1007/s12275-011-0519-1

Cited by 39 publications

(28 citation statements)

References 38 publications

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“…Knapp et al (2010) showed that resistance to beta-lactamases, tetracyclines, and erythromycins in soil collected over a period of 70~years, significantly increased. A similar approach was applied to study the prevalence of resistance genes in oral biofilms (Kim et al, 2011) and very recently to study fluoroquinolone resistance in fecal samples of Vietnamese children (Vien le et al, 2012). The main drawback to using targeted PCR-based metagenomics is that the results are skewed towards known resistance genes and mechanisms.…”

Section: Metagenomics To Characterize the Amr Reservoirmentioning

confidence: 99%

The human microbiome as a reservoir of antimicrobial resistance

Penders¹,

Stobberingh²,

Savelkoul³

et al. 2013

Front. Microbiol.

234

165

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The gut microbiota is amongst the most densely populated microbial ecosystem on earth. While the microbiome exerts numerous health beneficial functions, the high density of micro-organisms within this ecosystem also facilitates horizontal transfer of antimicrobial resistance (AMR) genes to potential pathogenic bacteria. Over the past decades antibiotic susceptibility testing of specific indicator bacteria from the microbiome, such as Escherichia coli, has been the method of choice in most studies. These studies have greatly enlarged our understanding on the prevalence and distribution of AMR and associated risk factors. Recent studies using (functional) metagenomics, however, highlighted the unappreciated diversity of AMR genes in the human microbiome and identified genes that had not been described previously. Next to metagenomics, more targeted approaches such as polymerase chain reaction for detection and quantification of AMR genes within a population are promising, in particular for large-scale epidemiological screening. Here we present an overview of the indigenous microbiota as a reservoir of AMR genes, the current knowledge on this “resistome” and the recent and upcoming advances in the molecular diagnostic approaches to unravel this reservoir.

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Section: Metagenomics To Characterize the Amr Reservoirmentioning

confidence: 99%

The human microbiome as a reservoir of antimicrobial resistance

Penders¹,

Stobberingh²,

Savelkoul³

et al. 2013

Front. Microbiol.

234

165

View full text Add to dashboard Cite

show abstract

“…In one study, 509 different bacteria were detected in periodontal samples, but only 29 of these were found among the 560 intestinal- or fecal bacteria isolated, illustrating this point (29). Furthermore, to our knowledge only narrow spectrum beta-lactamases, AmpC beta-lactamases or unspecified TEM-genes have been detected in plaque of patients with periodontitis or in plaque samples from the normal population (20, 30–32). The R. aquatilis with the ESBL gene bla RAHN-1/2 identified in the present material is thus, to our knowledge, a novelty.…”

Section: Discussionmentioning

confidence: 88%

Extended-spectrum beta-lactamase-producing bacteria are not detected in supragingival plaque samples from human fecal carriers of ESBL-producing Enterobacteriaceae

Søraas

Olsen

Sundsfjord

et al. 2014

Journal of Oral Microbiology

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BackgroundThe prevalence of infections caused by Cefotaximase-Munich (CTX-M)-type extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) has rapidly increased during the past 15 years. Enterobacteriaceae are commonly found in the gastrointestinal tract and long-term intestinal carriage is considered important for the spread of ESBL and as a source of clinical infections. Oral biofilm such as supragingival plaque is known to contain numerous antibiotic resistance determinants and may also represent a poorly investigated site for ESBL carriage and further spread.ObjectiveTo investigate possible carriage of ESBL-producing bacteria in supragingival plaque of known fecal carriers of these bacteria.DesignWe screened for the presence of aerobic and anaerobic ESBL-producing bacteria and bla CTX-M in supragingival plaque samples from healthy human adults with culture-verified fecal carriage of CTX-M-producing Escherichia coli. The presence or absence of Enterobacteriaceae and ESBL-producing bacteria in plaque samples was evaluated using culture-based methods and consensus CTX-M PCR.ResultsOral samples were obtained from 17 participants with known previous carriage of ESBL-producing E. coli. No ESBL-producing bacteria or ESBL genes were detected using culture-based and molecular methods. One colony of Rahnella aquatilis harboring the class A ESBL gene blaRAHN-1/2 was identified in an oral sample from one of the participants.ConclusionThis pilot study supports the notion that the presence of CTX-M-producing bacteria is uncommon in oral plaque of healthy human adult fecal carriers. Due to the limited number of persons tested, a low prevalence of oral ESBL-carriage in healthy adults or carriage in selected groups of patients cannot be excluded. To our knowledge, this is the first description of an R. aquatilis with the RAHN-1/2 gene in the oral cavity.

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“…Antibiotic therapy to treat gram-negative bacteria promotes growth of multiresistant bacteria in ulcers. Dental biofilms may serve as a reservoir of bacteria and antimicrobial resistance determinants [3]. Antibiotic-resistant bacteria, such as those that produce β-lactamase, are more prominent in periodontitis [4].…”

Section: Descriptionmentioning

confidence: 99%