Characterization of Anastomosis Groups of Binucleate<i>Rhizoctonia</i>Species Using Restriction Analysis of an Amplified Ribosomal RNA Gene

Cubeta, Marc A.; Echandi, E.; Abernethy, T.; Vilgalys, Rytas

doi:10.1094/phyto-81-1395

Cited by 212 publications

(107 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that RFLP analysis of rDNA is a useful technique for identification of some fungi (Cubeta et al, 1991;Chen, 1992;Ward and Akrofi, 1994;Erland, 1995), and that RAPD analysis is also useful for identification of fungi (Bentley et al, 1995;Theodore et al, 1995) and for investigation of the correlation of genetic variation with pathotype (Nicholson and Rezanoor, 1994). Nakada et al (1994) have reported that analysis of RFLPs of total DNA using arbitrarily chosen genomic clones as probe revealed clear differences among the most common plant pathogenic fungi, Bipolaris and Curvularia species, on gramineous plants.…”

Section: Discussionmentioning

confidence: 99%

Distribution of photo-induced and non-photo-induced sporulator physiotypes of Bipolaris oryzae in Japan

1997

View full text Add to dashboard Cite

The distribution of three sporulator physiotypes of Bipolaris oryzae, namely, photo-induced, and non-photo-induced (I) and (11), was investigated. Of 407 isolates, 99% belonged to the photo-induced type, in which conidial development was under photo-control of the antagonistic action of blue/UV-A and near-UV radiation mediated through the "mycochrome' system at conidiophore induction and conidiophore maturation stages. Of the remainder, 1 isolate belonged to the non-photo-induced (I) type, and 4 isolates belonged to the non-photo-induced (11) type. Conidial development in the former of these was photo-controlled by the "mycochrome' system at conidiophore maturation stage alone, while in the latter it was not affected by light conditions. No difference was found between the three physiotypes in restriction fragment length polymorphisms (RFLPs) of rDNA. However, random amplified polymorphic DNA (RAPD) revealed polymorphisms between photo-induced and non-photo-induced isolates and showed that non-photo-induced (I) and (ll) strains were clustered in the same group, suggesting that they are genetically close. Photo-induced sporulators of B. oryzae were confirmed to be widely distributed in paddy fields in Japan. Key WordsBipolaris oryzae; mycochrome, photo-control of conidiation; RAPD; RFLP.It has been reported that there are two sporulator physiotypes of fungi, photo-induced and non-photo-induced, based on the light-dark cycle necessary for induction of conidiophore formation (Leach, 1967;Kumagai, 1978;Tan, 1978). Light is required for the induction of conidiophore formation in the photo-induced sporulators, but not in the non-photo-induced sporulators. It has also been found that conidial development in certain fungi imperfecti such as Bipolaris oryzae (Breda de Haan) Shoem., Alternaria tomato (Cooke) Weber, A. cichorii Nattras, and Botrytis cinerea Pers.: Fr. is controlled by an antagonistic action of blue/UV-A and near-UV radiation mediated through the 'mycochrome' system at two developmental stages: induction of conidiophore formation and conidiophore maturation (see Kumagai, 1978;1988). Conidiophore formation in photo-induced sporulators of B. oryzae is induced by near-UV radiation, and conidia develop in the subsequent darkness. However, the inductive effect of near-UV radiation can be nullified by blue radiation applied immediately after inductive near-UV radiation. When conidiophores of photo-induced sporulators are exposed to blue radiation at a certain conidiophore maturation stage, they dedifferentiate into sterile conidiophores, and conidia do not form. However, the inhibitory effect of blue radiation on conidial development can be nullified by near-UV radiation applied immediately after blue radiation. The effects of blue and near-UV radiation can be repeatedly reversed, the final response depending on the last type of radiation administered. On the other hand, in a non-photo-induced strain of B. oryzae, conidiophore formation can be induced irrespective of light or dark conditions (Yamamura et al., 1978...

show abstract

Section: Discussionmentioning

confidence: 99%

Distribution of photo-induced and non-photo-induced sporulator physiotypes of Bipolaris oryzae in Japan

1997

View full text Add to dashboard Cite

show abstract

“…After a new centrifugation of 15 min at the same speed, the pellet was washed in cold ethanol 70%, centrifuged again for 2 min, and dried. It was finally resuspended in 200 µL of ddH 2 O. PCR amplification was performed with primers ITS1F and ITS4 (White et al, 1990;Gardes and Bruns, 1993) for the ITS region, while LR0R and LR5 (Vilgalys and Hester, 1990;Cubeta et al, 1991) were used to amplify the 28S rDNA region. PCR reactions were performed under a program consisting of a hot start at 95 °C for 5 min, followed by 35 cycles at 94 °C, 54 °C, and 72 °C (45, 30, and 45 s, respectively) and a final 72 °C step for 10 min.…”

Section: Methodsmentioning

confidence: 99%

A new species of Hygrophorus, H. yadigarii sp. nov. (Hygrophoraceae), with an isolated systematic position within the genus from the Colchic part of Turkey

Sesli¹,

Antonín²,

Contu³

2018

Turk J Bot

View full text Add to dashboard Cite

Uzun et al., 2017), and it is characterized by its tricholomatoid, collybioid, clitocyboid, or omphalinoid small to large, thin to fleshy, dry to very glutinous or viscid basidioma; a whitish, dull-colored, gray, brownish, yellowish, orange, or reddish dry to fairly sticky pileus; closely or widely spaced, adnate to decurrent, typically thick and waxy, generally whitish, sometimes yellowish or pinkish lamellae; a dry to glutinous, glabrous or fibrillose, generally pruinose or granulose stipe; an often thick, sometimes reddening or yellowing whitish content; and long, narrowly clavate basidia and smooth, hyaline, nonamyloid basidiospores (Singer, 1986; Boertmann, 1995;Young, 2005;Kovalenko, 2012).According to the monograph by Hesler and Smith (1963), section Hygrophorus is divided into 8 subsections due to the macromorphology of the basidioma: subsect. Chrysodontini Singer (with yellow granules on the basidioma), subsect. Pallidini A.H.Sm. & Hesler (basidioma slightly greasy), subsect. Hygrophorus (basidioma distinctly slimy), subsect. Erubescentes A.H.Sm. & Hesler (basidioma reddish), subsect. Fulvoincarnati A.H.Sm. & Hesler (basidioma more or less orange colored), subsect. Discoidei (Bataille) Konrad & Maubl. (basidioma yellowish, yellowbrown to reddish), subsect. Olivaceoumbrini Bataille (basidioma partly slimy and usually shiny), and subsect.

show abstract

“…The polymerase chain reaction (PCR) targeting ITS1-5.8S-ITS2 region of the rDNA was carried out using primers ITS1 and ITS4 (White et al 1990). The large ribosomal subunit (28S rDNA) was amplified using the primers pair LR0R-LR5 (Vilgalys & Hester 1990, Cubeta et al 1991. PCR amplifications were performed as described by Zitouni-Haouar et al (2015).…”

Section: Morphological Studiesmentioning

confidence: 99%

Genetic diversity of the genus Terfezia (Pezizaceae, Pezizales): New species and new record from North Africa

et al. 2018

View full text Add to dashboard Cite

Morphological and phylogenetic analyses of large ribosomal subunit (28S rDNA) and internal transcribed spacer (ITS rDNA) of Terfezia samples collected from several bioclimatic zones in Algeria and Spain revealed the presence of six distinct Terfezia species: T. arenaria, T. boudieri, T. claveryi; T. eliocrocae (reported here for the first time from North Africa), T. olbiensis, and a new species, T. crassiverrucosa sp. nov., proposed and described here, characterized by its phylogenetic position and unique combination of morphological characters. A discussion on the unresolved problems in the taxonomy of the spiny-spored Terfezia species is conducted after the present results.

show abstract

Characterization of Anastomosis Groups of BinucleateRhizoctoniaSpecies Using Restriction Analysis of an Amplified Ribosomal RNA Gene

Cited by 212 publications

References 0 publications

Distribution of photo-induced and non-photo-induced sporulator physiotypes of Bipolaris oryzae in Japan

Distribution of photo-induced and non-photo-induced sporulator physiotypes of Bipolaris oryzae in Japan

A new species of Hygrophorus, H. yadigarii sp. nov. (Hygrophoraceae), with an isolated systematic position within the genus from the Colchic part of Turkey

Genetic diversity of the genus Terfezia (Pezizaceae, Pezizales): New species and new record from North Africa

Contact Info

Product

Resources

About