Characterization of an Upstream Activation Sequence and Two Rox1p-responsive Sites Controlling the Induction of the Yeast HEM13 Gene by Oxygen and Heme Deficiency

Amillet, Jean-Michel; Buisson, Nicole; Labbe-Bois, Rosine

doi:10.1074/jbc.271.40.24425

Cited by 21 publications

(21 citation statements)

References 40 publications

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“…Still, salt or water addition sufficed to briefly repress HEM13 (Fig. 5A), a known oxygen-repressed gene (2). We conclude that the expression of this set of genes is exquisitely sensitive to addition of even trace amounts of oxygen.…”

Section: Resultsmentioning

confidence: 87%

Anaerobicity Prepares Saccharomyces cerevisiae Cells for Faster Adaptation to Osmotic Shock

Krantz

Nordlander

Valadi³

et al. 2004

Eukaryot Cell

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Yeast cells adapt to hyperosmotic shock by accumulating glycerol and altering expression of hundreds of genes. This transcriptional response of Saccharomyces cerevisiae to osmotic shock encompasses genes whose products are implicated in protection from oxidative damage. We addressed the question of whether osmotic shock caused oxidative stress. Osmotic shock did not result in the generation of detectable levels of reactive oxygen species (ROS). To preclude any generation of ROS, osmotic shock treatments were performed in anaerobic cultures. Global gene expression response profiles were compared by employing a novel twodimensional cluster analysis. The transcriptional profiles following osmotic shock under anaerobic and aerobic conditions were qualitatively very similar. In particular, it appeared that expression of the oxidative stress genes was stimulated upon osmotic shock even if there was no apparent need for their function. Interestingly, cells adapted to osmotic shock much more rapidly under anaerobiosis, and the signaling as well as the transcriptional response was clearly attenuated under these conditions. This more rapid adaptation is due to an enhanced glycerol production capacity in anaerobic cells, which is caused by the need for glycerol production in redox balancing. Artificially enhanced glycerol production led to an attenuated response even under aerobic conditions. These observations demonstrate the crucial role of glycerol accumulation and turgor recovery in determining the period of osmotic shock-induced signaling and the profile of cellular adaptation to osmotic shock.The ability to perceive and adapt to environmental changes is essential for all cells. Cellular adaptation to hyperosmotic shock in the yeast Saccharomyces cerevisiae requires accumulation of glycerol as compatible solute and is mediated by both transcriptional and metabolic alterations. Hyperosmotic shock evokes a broad transcriptional response, including that of several genes implicated in oxidative stress protection (8,17,25,28,39). In this work, we addressed the question of whether this is due to coregulation or due to osmotic shock resulting in oxidative stress. Such a link between osmotic shock and development of reactive oxygen species (ROS) could have specific regulatory and signaling roles, as suggested for liver cells (26,27). Moreover, it has recently been reported that the yeast osmosensing high-osmolarity glycerol (HOG) pathway can also be stimulated by oxidative stress treatments (4). To study a possible link between osmotic and oxidative stress in yeast, we utilized the ability of S. cerevisiae to grow in the absence of oxygen, precluding the development of ROS.Cells subjected to a hyperosmotic shock suffer loss of water and turgor pressure, transiently arrest proliferation, and recover by accumulating glycerol (reviewed in reference 18). Glycerol stimulates water uptake and restoration of turgor pressure, and the accumulation is controlled at three levels.

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Section: Resultsmentioning

confidence: 87%

Anaerobicity Prepares Saccharomyces cerevisiae Cells for Faster Adaptation to Osmotic Shock

Krantz

Nordlander

Valadi³

et al. 2004

Eukaryot Cell

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“…CPO encodes the enzyme coproporphyrinogen oxidase that catalyzes the sixth step in the heme biosynthetic pathway. Its transcription was induced 40-50-fold under conditions of oxygen or heme deficiency (Amillet et al, 1996;Blanco et al, 2005). Previous works have also shown that coproporphyrinogen oxidase activity became rate-limiting for heme production when its substrate oxygen is limiting.…”

Section: Discussionmentioning

confidence: 95%

“…Previous works have also shown that coproporphyrinogen oxidase activity became rate-limiting for heme production when its substrate oxygen is limiting. Cells responded to oxygen limitation by increasing the amount of the enzyme (Amillet et al, 1996;Blanco et al, 2005). Hence, coproporphyrinogen oxidase may play a crucial role in linking heme synthesis to the oxygen/heme-dependent control of gene expression.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Zhong

Wang

Zhang

et al. 2009

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…Transcript levels of HEM13, the rate-limiting step of heme biosynthesis (57), were reduced in response to seven drug treatments and, again, in none of the deletion strains. HEM13 is repressed by heme and oxygen (1,22), raising the possibility that levels of one or both are elevated following treatment. This is consistent with the hypothesis of increased intracellular oxygen suggested by the responses of anaerobiosis-induced and oxygen stress genes described above.…”

Section: Erg12 Are2 Coq7 [Also Referred To As Cat5] Far3 Fig1 Hementioning

confidence: 99%

Genome-Wide Expression Patterns in Saccharomyces cerevisiae : Comparison of Drug Treatments and Genetic Alterations Affecting Biosynthesis of Ergosterol

Bammert¹,

Fostel²

2000

Antimicrob Agents Chemother

206

175

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Enzymes in the ergosterol-biosynthetic pathway are the targets of a number of antifungal agents including azoles, allylamines, and morpholines. In order to understand the response of Saccharomyces cerevisiae to perturbations in the ergosterol pathway, genome-wide transcript profiles following exposure to a number of antifungal agents targeting ergosterol biosynthesis (clotrimazole, fluconazole, itraconazole, ketoconazole, voriconazole, terbinafine, and amorolfine) were obtained. These profiles were compared to the transcript profiles of strains containing deletions of one of the late-stage ergosterol genes: ERG2, ERG5, or ERG6. A total of 234 genes were identified as responsive, including the majority of genes from the ergosterol pathway. Expression of several responsive genes, including ERG25, YER067W, and YNL300W, was also monitored by PCR over time following exposure to ketoconazole. The kinetics of transcriptional response support the conditions selected for the microarray experiment. In addition to ergosterol-biosynthetic genes, 36 mitochondrial genes and a number of other genes with roles related to ergosterol function were responsive, as were a number of genes responsive to oxidative stress. Transcriptional changes related to heme biosynthesis were observed in cells treated with chemical agents, suggesting an additional effect of exposure to these compounds. The expression profile in response to a novel imidazole, PNU-144248E, was also determined. The concordance of responsive genes suggests that this compound has the same mode of action as other azoles. Thus, genome-wide transcript profiles can be used to predict the mode of action of a chemical agent as well as to characterize expression changes in response to perturbation of a metabolic pathway.

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Characterization of an Upstream Activation Sequence and Two Rox1p-responsive Sites Controlling the Induction of the Yeast HEM13 Gene by Oxygen and Heme Deficiency

Cited by 21 publications

References 40 publications

Anaerobicity Prepares Saccharomyces cerevisiae Cells for Faster Adaptation to Osmotic Shock

Anaerobicity Prepares Saccharomyces cerevisiae Cells for Faster Adaptation to Osmotic Shock

Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Genome-Wide Expression Patterns in Saccharomyces cerevisiae : Comparison of Drug Treatments and Genetic Alterations Affecting Biosynthesis of Ergosterol

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