Characterization of an unexpected bioemulsifier from spent yeast obtained from Thai traditional liquor distillation

Dikit, Paweena; Methacanon, Pawadee; Visessanguan, Wonnop; H‐Kittikun, Aran; Maneerat, Suppasil

doi:10.1016/j.ijbiomac.2010.06.013

Cited by 19 publications

(10 citation statements)

References 28 publications

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“…Yeasts have been traditionally used in fermentation processes, as food flavoring and enrichment ingredients as yeast extract and autolysate (Pacheco et al, 1997), and are generally recognized as safe -GRAS (Briggs et al, 2004). Due to this, for inactivated yeast derivatives, new applications have been explored as nutritive complements and ingredients for formulations in food industry (Chae et al, 2001;Dikit et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Nutritional ingredients from spent brewer's yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process

Amorim

Pereira

Gomes

et al. 2016

Journal of Food Engineering

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Section: Introductionmentioning

confidence: 99%

Nutritional ingredients from spent brewer's yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process

Amorim

Pereira

Gomes

et al. 2016

Journal of Food Engineering

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“…A broad band was observed around 3428 cm )1 and a weak C-H stretching band at 2923 cm )1 , exhibiting characteristic for O-H content, typical of polysaccharides. Because of low protein content, N-H vibration generally expected at 3400 cm )1 could be overlapped by C-H stretching vibration (Dikit et al 2010). Further, an asymmetrical stretching peak was noticed at 1540-1698 cm )1 and a weak symmetrical stretching peak near 1360-1455 cm )1 , suggesting the presence of carboxyl groups (Gonzaga et al 2005).…”

Section: Characterization Of the Bioemulsifiermentioning

confidence: 97%

Characterization and emulsifying property of a novel bioemulsifier by Aeribacillus pallidus YM-1

Chang

et al. 2012

Journal of Applied Microbiology

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Aims: Biosurfactants and bioemulsifiers commonly have the advantages of biodegradability, low toxicity, selectivity and biocompatibility over chemically synthesized surfactants. The goal of the study is to present a novel bioemulsifier with great application potential. Methods and Results: Aeribacillus pallidus YM‐1, isolated from crude oil contaminated soil, was found to produce a novel high molecular bioemulsifier with an emulsification index of 60 ± 1% without remarkable surface tension reduction (45·7 ± 0·1 mN m−1). The number‐average molecular weight was determined as 526 369 Da by gel permeation chromatography analysis. Bioemulsifier was subjected to FT‐IR and a complex of carbohydrates (41·1%), lipids (47·6%) and proteins (11·3%) was determined. Conclusions: The bioemulsifier of A. pallidus YM‐1 was isolated from the glucose‐based culture medium and characterized with the help of chemical analytical techniques. The bioemulsifier exhibited a promising emulsifying property for biotechnology application potential in bioremediation and microbial enhanced oil recovery. Significance and Impact of the Study: This is the first report of the bioemulsifier production by A. pallidus. The potential emulsifying activity of the bioemulsifier in the present study may be explored in various biotechnological and industrial applications.

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Section: Evaluation Of Bsmentioning

confidence: 99%

“…The homogeneity of EBS was confirmed by UV/VIS spectroscopy [25] and HPLC [26]. The ultraviolet absorbance spectrum of EBS [25] at a range of 190-400 nm (UV-VIS Spectrophotometer, PERKIN-ELMER) was analyzed. Further, EBS was examined with HPLC (Shimadzu, USA) using, a reverse-phase column (Lichrosorb C18-5 μm; Merck, Germany) and UV assay detector (280).…”

Section: Evaluation Of Bsmentioning

confidence: 99%

Biphasic Liquid-Liquid Extraction of Biosurfactant from Lactobacillus delbrueckii

Goyal

Singh²

2022

Braz. arch. biol. technol.

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The present work is focused mainly on optimizing the extraction of glycoprotein-biosurfactant (BS) produced by Lactobacillus delbrueckii in a biphasic liquid-liquid extraction. The production yield of BS is significantly affected by extraction strategies instead of the whole fermentation process, so it becomes a straightforward approach to enhance BS yield while optimizing various extraction parameters. The tailoring of process parameters for BS extraction was achieved by OFAT (one factor at a time) strategy and partition coefficient (Kd) served as the calculation factor for extraction. The optimal yield of BS (5+0.1 g/L) from CFB (from cell-free broth) was achieved by solvents; chloroform, methanol, and, butanol, 1:2:1 (v/v), from cell-free broth (CFB), 30% (v/v), at pH 3.5, temperature 37°C after extraction time of 60 min. Under optimized conditions, the extraction yield was 78.5% higher and subsequently, a co-current, and counter-current system enhanced the extraction yield by 16% (5.8g/L) and 20% (6.0g/L) respectively. The purity of extracted BS (EBS) was confirmed by UV/Visible spectroscopy and HPLC (High-performance liquid chromatography). The concentration-dependent activity profile of EBS analyzed by ODA (oil displacement area; 50.24+0.3 cm 2 ), DCD (drop collapse diameter; 1.1+0.3 cm), and EI24 (73+0.3%), exhibited enhancement by 60, 52, and 55% respectively as compared to control. Thin-layer chromatography (TLC), FTIR (Fourier Transform Infra-Red) and NMR (Nuclear Magnetic Resonance) techniques confirmed the polymeric glycoprotein (65:35 protein: carbohydrates %) nature of BS.

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Characterization of an unexpected bioemulsifier from spent yeast obtained from Thai traditional liquor distillation

Cited by 19 publications

References 28 publications

Nutritional ingredients from spent brewer's yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process

Nutritional ingredients from spent brewer's yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process

Characterization and emulsifying property of a novel bioemulsifier by Aeribacillus pallidus YM-1

Biphasic Liquid-Liquid Extraction of Biosurfactant from Lactobacillus delbrueckii

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