Characterization of an uncharacterized aldo-keto reductase gene from peach and its role in abiotic stress tolerance

Kanayama, Yoshinori; Mizutani, Ryosuke; Yaguchi, Shino; Hojo, Ayano; Ikeda, Hiroyasu; Nishiyama, Manabu; Kanahama, Koki

doi:10.1016/j.phytochem.2014.04.008

Cited by 33 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Glucose, fructose and sucrose contents of tomato fruits were measured as described by Kanayama et al . (). Briefly, small pieces (~5 mm 3 in size) of red‐ripened fruits were extracted with ethanol at 80 °C three times.…”

Section: Methodsmentioning

confidence: 97%

A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit‐specific expression of a single bZIP transcription factor gene

Sagor

Berberich

Tanaka

et al. 2015

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

SummaryEnhancement of sugar content and sweetness is desirable in some vegetables and in almost all fruits; however, biotechnological methods to increase sugar content are limited. Here, a completely novel methodological approach is presented that produces sweeter tomato fruits but does not have any negative effects on plant growth. Sucrose-induced repression of translation (SIRT), which is mediated by upstream open reading frames (uORFs), was initially reported in Arabidopsis AtbZIP11, a class S basic region leucine zipper (bZIP) transcription factor gene. Here, two AtbZIP11 orthologous genes, SlbZIP1 and SlbZIP2, were identified in tomato (Solanum lycopersicum). SlbZIP1 and SlbZIP2 contained four and three uORFs, respectively, in the cDNA 5 0 -leader regions. The second uORFs from the 5 0 cDNA end were conserved and involved in SIRT. Tomato plants were transformed with binary vectors in which only the main open reading frames (ORFs) of SlbZIP1 and SlbZIP2, without the SIRTresponsive uORFs, were placed under the control of the fruit-specific E8 promoter. Growth and morphology of the resulting transgenic tomato plants were comparable to those of wildtype plants. Transgenic fruits were approximately 1.5-fold higher in sugar content (sucrose/ glucose/fructose) than nontransgenic tomato fruits. In addition, the levels of several amino acids, such as asparagine and glutamine, were higher in transgenic fruits than in wild-type fruits. This was expected because SlbZIP transactivates the asparagine synthase and proline dehydrogenase genes. This 'sweetening' technology is broadly applicable to other plants that utilize sucrose as a major translocation sugar.

show abstract

Section: Methodsmentioning

confidence: 97%

A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit‐specific expression of a single bZIP transcription factor gene

Sagor

Berberich

Tanaka

et al. 2015

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Codeinone reductases display the conserved AKR catalytic tetrad (D51, Y56, K86, H119) and mediate activity via an α/β barrel motif binding NADP(H) (Jez et al ., ). Plant AKRs play diverse physiological roles including in defense and the stress response, biosynthesis of osmolytes and membrane transport (Tang et al ., ; Kanayama et al ., ; Turóczy et al ., ; Vemanna et al ., ). Currently, COR and 1,2‐dehydroreticuline reductase (DRR) are the two known AKRs involved in BIA metabolism (Unterlinner et al ., ; Farrow et al ., ; Winzer et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Codeinone reductase isoforms with differential stability, efficiency and product selectivity in opium poppy

et al. 2018

View full text Add to dashboard Cite

Codeinone reductase (COR) catalyzes the reversible NADPH-dependent reduction of codeinone to codeine as the penultimate step of morphine biosynthesis in opium poppy (Papaver somniferum). It also irreversibly reduces neopinone, which forms by spontaneous isomerization in aqueous solution from codeinone, to neopine. In a parallel pathway involving 3-O-demethylated analogs, COR converts morphinone to morphine, and neomorphinone to neomorphine. Similar to neopine, the formation of neomorphine by COR is irreversible. Neopine is a minor substrate for codeine O-demethylase (CODM), yielding morphine. In the plant, neopine levels are low and neomorphine has not been detected. Silencing of CODM leads to accumulation of upstream metabolites, such as codeine and thebaine, but does not result in a shift towards higher relative concentrations of neopine, suggesting a mechanism in the plant for limiting neopine production. In yeast (Saccharomyces cerevisiae) engineered to produce opiate alkaloids, the catalytic properties of COR lead to accumulation of neopine and neomorphine as major products. An isoform (COR-B) was isolated from opium poppy chemotype Bea's Choice that showed higher catalytic activity than previously characterized CORs, and it yielded mostly neopine in vitro and in engineered yeast. Five catalytically distinct COR isoforms (COR1.1-1.4 and COR-B) were used to determine sequence-function relationships that influence product selectivity. Biochemical characterization and site-directed mutagenesis of native COR isoforms identified four residues (V25, K41, F129 and W279) that affected protein stability, reaction velocity, and product selectivity and output. Improvement of COR performance coupled with an ability to guide pathway flux is necessary to facilitate commercial production of opiate alkaloids in engineered microorganisms.

show abstract

“…Previous studies have assessed the performance of AKRs producing sugar alcohol as a primary function. For example, either celery or peach aldo-keto reductase enzyme was ectopically over-expressed in transgenic Arabidopsis, which lead to higher sugar alcohol content and salt tolerance was also improved (Zhifang et al 2003;Kanayama et al 2014). It has to be noted that apart from AKR overexpression, improved salt tolerance can be based on either osmoprotection (Shekhawat et al 2011) or on overproducing antioxidant enzymes (Hoshida et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Improved reactive aldehyde, salt and cadmium tolerance of transgenic barley due to the expression of aldo–keto reductase genes

et al. 2016

View full text Add to dashboard Cite

Under various stress conditions, plant cells are exposed to oxidative damage which triggers lipid peroxidation. Lipid peroxide breakdown products include protein crosslinking reactive aldehydes. These are highly damaging to living cells. Stress-protective aldo-keto reductase (AKR) enzymes are able to recognise and modify these molecules, reducing their toxicity.AKRs not only modify reactive aldehydes but may synthesize osmoprotective sugar alcohols as well. The role of these mixed function enzymes was investigated under direct reactive aldehyde, heavy metal and salt stress conditions. Transgenic barley (Hordeum vulgare L.) plants constitutively expressing AKR enzymes derived from either thale cress (Arabidopsis thaliana) (AKR4C9) or alfalfa (Medicago sativa) (MsALR) were studied. Not only AKR4C9 but MsALR expressing plants were also found to produce more sorbitol than the nontransgenic (WT) barley. Salinity tolerance of genetically modified (GM) plants improved, presumably as a consequence of the enhanced sorbitol content. The MsALR enzyme expressing line (called 51) exhibited almost no symptoms of salt stress. Furthermore, both transgenes were shown to increase reactive aldehyde (glutaraldehyde) tolerance. Transgenic plants also exhibited better cadmium tolerance compared to WT, which was considered to be an effect of the reduction of reactive aldehyde molecules. Transgenic barley expressing either thale cress or alfalfa derived enzyme showed improved heavy metal and salt tolerance. Both can be explained by higher detoxifying and sugar alcohol producing activity. Based on the presented data, we consider AKRs as very effective stress-protective enzymes and their genes provide promising tools in the improvement of crops through gene technology.Key words: transgenic plant, lipid peroxidation, sorbitol, heavy metal, salt stress 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

show abstract

Characterization of an uncharacterized aldo-keto reductase gene from peach and its role in abiotic stress tolerance

Cited by 33 publications

References 41 publications

A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit‐specific expression of a single bZIP transcription factor gene

A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit‐specific expression of a single bZIP transcription factor gene

Codeinone reductase isoforms with differential stability, efficiency and product selectivity in opium poppy

Improved reactive aldehyde, salt and cadmium tolerance of transgenic barley due to the expression of aldo–keto reductase genes

Contact Info

Product

Resources

About