Characterization of an Lyt-2+ alloreactive cytotoxic T cell clone specific for H-2Db that cross-reacts with I-Ek.

Schilham, Marco W.; Lang, Rosemarie; Benner, R.; Zinkernagel, R M; Hengartner, Hans

doi:10.4049/jimmunol.137.9.2748

The Journal of Immunology

1986

DOI: 10.4049/jimmunol.137.9.2748

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Characterization of an Lyt-2+ alloreactive cytotoxic T cell clone specific for H-2Db that cross-reacts with I-Ek.

Marco W. Schilham¹,

Rosemarie Lang²,

R. Benner³

et al.

Abstract: An alloreactive cytotoxic T cell clone (433) possessing the L3T4-, Lyt-2+ phenotype is described that shows a double specificity. It has been derived from unprimed BALB/c (H-2d) spleen cells by repetitive in vitro restimulation with C57BL/6(H-2b) cells. The specificity of clone 433 was determined in cytotoxicity and proliferation experiments. One specificity was for the class I antigen H-2Db and the second was for the class II antigen I-Ek. Inhibition of cytotoxicity with monoclonal antibodies confirmed these … Show more

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Cited by 33 publications

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Existence of mature human CD4⁺ T cells with genuine class I restriction

1992

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A humanT cell receptor (TcR) u/p CD4+CD8-T cell clone (R416) is reactive with the minor histocompatibility antigen H-Y in the context of major histocompatibility complex (MHC) class I and not class I1 molecules. Therewith clone R416 violates the so-called specificity association of mature TcR a@+ Tcells. R416 displays H-Y-specific, HLA-A2-restricted proliferation as well as cytotoxicity in vitro. Its fine specificity is identical to that of a classical H-Y-reactive CD4-CD8+ MHC class I-restricted CTL clone, showing that CTL expressing either CD4 or CD8 can display identical antigenic specificities. Exploiting the MHC class I restriction of this CD4+ Tcell clone, it was found that interaction of CD4 with non-TcR-bound MHC class I1 molecules does not contribute to antigen specific activation of these CD4+ T cells.This coreceptor-mismatched T cell clone was not generated in vitro but obtained by expansion of CD8-depleted peripheral blood mononuclear cells of a female who had been immunized against H-Y. The existence of such MHC class I-restricted mature TcR dp+ T cells expressing CD4and not CD8 is relevant because it indicates that the generally accepted model for thymic selection, in which the TcR specificity alone determines CD4/CD8 expression of mature thymocytes, may not be absolute.

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Existence of mature human CD4⁺ T cells with genuine class I restriction

1992

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CD4‐class II major histocompatibility complex interaction does not enhance killing by a class I‐restricted CD4⁺CD8⁺ cytotoxic T cell clone

et al. 1992

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As unusual tumor-specific cytotoxic T lymphocyte (CTL) clone was isolated which expressed both CD4 and CD8 molecules. The target cells for this CTL can be induced to express either class I major histocompatibility complex (MHC) alone (with dimethylsulfoxide) or both class I and class II MHC (with interferon-gamma). Lysis of the tumor target depends on expression of class I MHC molecules, but does not require expression of class II MHC molecules. Furthermore, the lysis of target cells expressing both class I and class II is inhibited only by antibodies to class I (Kd), and not by antibodies to class II, demonstrating that the T cell receptor is class I restricted. We have used this CTL to assess the role of the interaction between CD4 and class II MHC in the absence of a class II-restricted T cell receptor. Our data indicate that CD4-class II interaction does not contribute to recognition by T cells in the absence of binding of the T cell receptor to class II molecules.

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Alloreactive cytotoxic T cells can develop and function in mice lacking both CD4 and CD8

et al. 1993

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Using the technique of homologous recombination in embryonic stem cells, a mouse strain without functional CD4 and CD8 genes has been generated. Surprisingly, these mice contain significant numbers of alpha beta T cells. Although mice deficient for CD8 only do not show any cytotoxic response when their T cells are stimulated with either alloantigen or viral antigen, the CD4-8- mice do generate alloreactive cytotoxic T cells. These cytotoxic T cells bear the alpha beta T cell receptor and recognize major histocompatibility complex class I antigens. In addition fully allogeneic skin transplants were rejected but skin transplants expressing only minor transplantation antigens were not. Virus-specific cytotoxic T lymphocytes were also not detected. It seems that alloreactive cytotoxic T cells can be induced and exert their effector function in vitro and in vivo in the absence of CD8, and that they can develop and mature in vivo without the CD8 molecule or the signals it might provide.

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Characterization of an Lyt-2+ alloreactive cytotoxic T cell clone specific for H-2Db that cross-reacts with I-Ek.

Cited by 33 publications

References 0 publications

Existence of mature human CD4⁺ T cells with genuine class I restriction

Existence of mature human CD4⁺ T cells with genuine class I restriction

CD4‐class II major histocompatibility complex interaction does not enhance killing by a class I‐restricted CD4⁺CD8⁺ cytotoxic T cell clone

Alloreactive cytotoxic T cells can develop and function in mice lacking both CD4 and CD8

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Characterization of an Lyt-2+ alloreactive cytotoxic T cell clone specific for H-2Db that cross-reacts with I-Ek.

Cited by 33 publications

References 0 publications

Existence of mature human CD4+ T cells with genuine class I restriction

Existence of mature human CD4+ T cells with genuine class I restriction

CD4‐class II major histocompatibility complex interaction does not enhance killing by a class I‐restricted CD4+CD8+ cytotoxic T cell clone

Alloreactive cytotoxic T cells can develop and function in mice lacking both CD4 and CD8

Contact Info

Product

Resources

About

Existence of mature human CD4⁺ T cells with genuine class I restriction

Existence of mature human CD4⁺ T cells with genuine class I restriction

CD4‐class II major histocompatibility complex interaction does not enhance killing by a class I‐restricted CD4⁺CD8⁺ cytotoxic T cell clone