Characterization of an inhibitor-resistant endo-1,4-β-mannanase from the gut microflora metagenome of Hermetia illucens

Song, Jae-Eun; Kim, Su-Yeon; Kim, Dae-Hyuk; Lee, Youngseok; Sim, Ji Hyun; Hahn, Bum‐Soo; Lee, Chang-Muk

doi:10.1007/s10529-018-2596-2

Cited by 10 publications

(3 citation statements)

References 34 publications

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“…The effect of temperature (20~70 • C), pH (3~10), 5 mmol/L metal ions (EDTA, Na + , K + , Ca 2+ , Mn 2+ , Mg 2+ , Fe 2+ , Zn 2+ , Cu 2+ , Al 3+ , Fe 3+ ) and kinetic parameters (K m ) of the reFAEs were determined as described by Song et al [17].…”

Section: Biochemical Characterization Of Refaesmentioning

confidence: 99%

Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing

et al. 2021

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Hydrolytic activity for chlorogenic acid (CGA) has been recognized as an important side activity of some types of ferulic acid esterases. The purpose of this work was to enhance the efficient expression of ferulic acid esterase (FAE) and to explore its application in the processing of sunflower seed. Two novel FAEs from Aspergillus aculeatus (AaSD14) were expressed in genetically engineered E. coli BL21 (DE3), and their properties, including temperature, pH, metal ions and substrate specificity, were characterized after purification. Competitive CGA hydrolysis activity was observed in these recombined ferulic acid esterases (reFAEs) with reFAE1 of 246.37 U/g and reFAE2 of 340.95 U/g, which were 56.6 and 78.4 times higher than that of the wild strain (4.35 U/g), respectively. Meanwhile, the fermentation cycle was greatly shortened to 2.0 d. These reFAEs were recognized as type C FAE through substrate specificity assays. Treatment of sunflower seed protein (SSP) using reFAE2 resulted in a remarkable color change, from green to milk-white, confirming the activity of CGA biodegradation. Therefore, it shows certain potential in the processing of sunflower seed and other related foodstuffs.

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Section: Biochemical Characterization Of Refaesmentioning

confidence: 99%

Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing

et al. 2021

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“…Little is known about the microbial metabolism taking place in the larval guts, since past studies have mostly focused on clarifying phylogenetic dynamics in relation to larval diet. Detailed investigations of metabolic processes taking place in larvae and attempts to exploit them for biotechnological applications are sparse (Lee et al, 2018;Song et al, 2018;Zhan et al, 2019). Tools imputing metagenomes to 16S rRNA gene-based amplicon data such as PICRUSt (Langille et al, 2013) or Tax4Fun (Aßhauer et al, 2015) have been used to provide insights into functional genes.…”

Section: Introductionmentioning

confidence: 99%

Impact of Processed Food (Canteen and Oil Wastes) on the Development of Black Soldier Fly (Hermetia illucens) Larvae and Their Gut Microbiome Functions

et al. 2021

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Canteens represent an essential food supply hub for educational institutions, companies, and business parks. Many people in these locations rely on a guaranteed service with consistent quality. It is an ongoing challenge to satisfy the demand for sufficient serving numbers, portion sizes, and menu variations to cover food intolerances and different palates of customers. However, overestimating this demand or fluctuating quality of dishes leads to an inevitable loss of unconsumed food due to leftovers. In this study, the food waste fraction of canteen leftovers was identified as an optimal diet for black soldier fly (Hermetia illucens) larvae based on 50% higher consumption and 15% higher waste reduction indices compared with control chicken feed diet. Although the digestibility of food waste was nearly twice as high, the conversion efficiency of ingested and digested chicken feed remains unparalleled (17.9 ± 0.6 and 37.5 ± 0.9 in CFD and 7.9 ± 0.9 and 9.6 ± 1.0 in FWD, respectively). The oil separator waste fraction, however, inhibited biomass gain by at least 85% and ultimately led to a larval mortality of up to 96%. In addition to monitoring larval development, we characterized physicochemical properties of pre- and post-process food waste substrates. High-throughput amplicon sequencing identified Firmicutes, Proteobacteria, and Bacteroidota as the most abundant phyla, and Morganella, Acinetobacter, and certain Lactobacillales species were identified as indicator species. By using metagenome imputation, we additionally gained insights into the functional spectrum of gut microbial communities. We anticipate that the results will contribute to the development of decentralized waste-management sites that make use of larvae to process food waste as it has become common practice for biogas plants.

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“…Little is known about the microbial metabolism taking place in the larval guts, since past studies have mostly focused on clarifying phylogenetic dynamics in relation to larval diet. Detailed investigations of metabolic processes taking place in larvae and attempts to exploit them for biotechnological applications are sparse Song et al, 2018;Zhan et al, 2019). Tools imputing metagenomes to 16S rRNA gene-based amplicon data such as PICRUSt or Tax4Fun (Aßhauer et al, 2015) have been used to provide insights into functional genes.…”

Section: Original Research Introductionmentioning

confidence: 99%

Microbial Dynamics During Industrial Rearing And Processing Of Insects

2022

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Characterization of an inhibitor-resistant endo-1,4-β-mannanase from the gut microflora metagenome of Hermetia illucens

Abstract: ManEM17 is an attractive candidate for mannan degradation under the high-organic-solvent and protein-denaturing processes in food and feed industries.

Cited by 10 publications

References 34 publications

Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing

Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing

Impact of Processed Food (Canteen and Oil Wastes) on the Development of Black Soldier Fly (Hermetia illucens) Larvae and Their Gut Microbiome Functions

Microbial Dynamics During Industrial Rearing And Processing Of Insects

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