Characterization of an Immortalized Human Vaginal Epithelial Cell Line

Rajan, Nithya; Pruden, Denise L.; Kaznari, Hattori; Cao, Qing; Anderson, Byron; Schaeffer, Anthony J.

doi:10.1097/00005392-200002000-00061

The Journal of Urology

2000

DOI: 10.1097/00005392-200002000-00061

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Characterization of an Immortalized Human Vaginal Epithelial Cell Line

Nithya Rajan¹,

Denise L. Pruden²,

Hattori Kaznari³

et al.

Abstract: This model system should facilitate studies on the interaction of pathogens with vaginal mucosal cells, an essential step in the progression of ascending UTIs in women.

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2022

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Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip

Mahajan

Doherty

et al. 2022

Preprint

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BackgroundA dominance of non-iners Lactobacillus species in the vaginal microbiome is optimal and strongly associated with gynecological and obstetric health, while the presence of diverse obligate or facultative anaerobic bacteria and a paucity in Lactobacillus species, similar to communities found in bacterial vaginosis (BV), is considered non-optimal and associated with adverse health outcomes. Various therapeutic strategies are being explored to modulate the composition of the vaginal microbiome; however, there is no human model that faithfully reproduces the vaginal epithelial microenvironment for preclinical validation of potential therapeutics or testing hypotheses about vaginal epithelium-microbiome interactions.ResultsHere, we describe an organ-on-a-chip (Organ Chip) microfluidic culture model of the human vaginal mucosa (Vagina Chip) that is lined by hormone-sensitive, primary vaginal epithelium interfaced with underlying stromal fibroblasts, which sustains a low physiological oxygen concentration in the epithelial lumen. We show that the Vagina Chip can be used to assess colonization by optimal L. crispatus consortia as well as non-optimal Gardnerella vaginalis-containing consortia, and to measure associated host innate immune responses. Co-culture of the L. crispatus consortia was accompanied by maintenance of epithelial cell viability, accumulation of D- and L-lactic acid, maintenance of a physiologically relevant low pH, and down regulation of proinflammatory cytokines. In contrast, co-culture of G. vaginalis-containing consortia in the Vagina Chip resulted in epithelial cell injury, a rise in pH, and upregulation of proinflammatory cytokines.ConclusionThis study demonstrates the potential of applying human Organ Chip technology to create a preclinical model of the human vaginal mucosa that can be used to better understand interactions between the vaginal microbiome and host tissues, as well as to evaluate the safety and efficacy of live biotherapeutics products.

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Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip

Mahajan

Doherty

et al. 2022

Preprint

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show abstract

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Characterization of an Immortalized Human Vaginal Epithelial Cell Line

Abstract: This model system should facilitate studies on the interaction of pathogens with vaginal mucosal cells, an essential step in the progression of ascending UTIs in women.

Cited by 1 publication

References 18 publications

Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip

Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip

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