Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

Nambiar, Reshma B.; Sellamuthu, Periyar Selvam; Perumal, Anand Babu; Sadiku, Rotimi; Phiri, Goitse; Jayaramudu, J.

doi:10.1016/j.procbio.2018.07.018

Cited by 66 publications

(30 citation statements)

References 43 publications

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“…The FTIR analysis showed that majority of the functional groups in the isolated EPS belonged to alkanes, alkynes, carboxylic and alcoholic groups. The results of FTIR and NMR analyses obtained by previous studies [33][34][35] were in conformity with our results. The EPS composition consisted of carbohydrates with some amounts of proteins and sugar acids, and also traces of nucleic acids.…”

Section: Discussionsupporting

confidence: 93%

Exploration of an Extracellular Polymeric Substance from Earthworm Gut Bacterium (Bacillus licheniformis) for Bioflocculation and Heavy Metal Removal Potential

Banerjee

Sarkar

et al. 2020

Applied Sciences

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The present study shows the potential of an extracellular polymeric substance (EPS) produced by Bacillus licheniformis strain KX657843 isolated from earthworm (Metaphire posthuma) gut in the sorption of Cu(II) and Zn(II) and in flocculation. After harvesting bacterial cells from sucrose supplemented denitrifying culture medium, the EPS was extracted following ethanolic extraction method. The Fourier Transform Infrared Spectroscopy (FTIR) and 1H and 13C Nuclear Magnetic Resonance (NMR) of EPS revealed its functional groups, electronegative constituents, unsaturated carbon, and carbonyl groups. The negatively charged functional groups of carbohydrates and protein moiety of the EPS endowed it with heavy metal binding capacity through electrostatic interactions. The highest flocculation activity (83%) of EPS was observed at 4 mg L−1 and pH 11. The metal sorption by EPS increased with increasing pH. At pH 8, the EPS was able to remove 86 and 81% Cu(II) and Zn(II), respectively, from a 25 mg L−1 metal solution. 94.8% of both the metals at 25 mg L−1 metal solutions were removed by EPS at EPS concentration of 100 mg L−1. From Langmuir isotherm model, the maximum sorption capacities of EPS were calculated to be 58.82 mg g−1 for Cu(II) and 52.45 mg g−1 for Zn(II). The bacterial EPS showed encouraging flocculating and metal sorption properties. The potential to remove Cu(II) and Zn(II) implies that the EPS obtained from the earthworm gut bacteria can be used as an effective agent for environmental remediation of heavy metals and in bioflocculation.

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Section: Discussionsupporting

confidence: 93%

Exploration of an Extracellular Polymeric Substance from Earthworm Gut Bacterium (Bacillus licheniformis) for Bioflocculation and Heavy Metal Removal Potential

Banerjee

Sarkar

et al. 2020

Applied Sciences

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“…In the present study, we used ATR-FTIR to depict changes in bacterial consortium exposed to gamma radiation. Our results showed that both proteins and carbohydrates in the cell membrane changed upon exposure of bacteria to increased doses of gamma radiation, changes in peaks correlated to lipids were not obvious, and this is in agreement with EPS fingerprint as reported by Nambiara et al (2018). ATR-FTIR spectroscopy is considered to be a comprehensive and sensitive method for detecting molecular changes in intact cells (Salman et al, 2010).…”

Section: Discussionsupporting

confidence: 89%

Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation

Selim

Maghrawy

Fathy

et al. 2020

Journal of Radiation Research and Applied Sciences

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The aim of the present work was to increase bacterial adhesion on anode via inducing membrane modifications to enhance textile wastewater treatment in Microbial Fuel Cell (MFC). Real textile wastewater was used in mediator-less MFCs for bacterial enrichment. The enriched bacteria were pre-treated by exposure to 1 KGy gamma radiation and were tested in MFC setup. Bacterial cell membrane permeability and cell membrane charges were measured using noninvasive dielectric spectroscopy measurements. The results show that pre-treatment using gamma radiation resulted in biofilm formation and increased cell permeability and exopolysaccharide production; this was reflected in both MFC performance (average voltage 554.67 mV) and decolorization (96.42%) as compared to 392.77 mV and 60.76% decolorization for non-treated cells. At the end of MFC operation, cytotoxicity test was performed for treated wastewater using a dermal cell line, the results obtained show a decrease in toxicity from 24.8 to 0 (v/v%) when cells were exposed to gamma radiation. Fourier-transform infrared (FTIR) spectroscopy showed an increase in exopolysaccharides in bacterial consortium exposed to increasing doses of gamma radiation suggesting that gamma radiation increased exopolysaccharide production, providing transient media for electron transfer and contributing to accelerating MFC performance. Modification of bacterial membrane prior to MFC operation can be considered highly effective as a pre-treatment tool that accelerates MFC performance.

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“…Furthermore, fermented meat, fruits, and dairy products were demonstrated to be good sources for the isolation of Leuconostoc strains with interesting capacities to produce exopolysaccharides. In the studies carried out with human breast milk [70,[113][114][115][116], Enterococcus faecalis and Lactobacillus spp. strains yielded amounts of EPS ranging from 380 to 737.3 mg/L.…”

Section: Capacity Of Presumptive Lactic Acid Bacteria To Produce Exopmentioning

confidence: 99%

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

et al. 2020

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The occurrence of inflammatory responses in humans is frequently associated with food intolerances and is likely to give rise to irritable bowel disease. The use of conventional or unconventional flours to produce gluten-free baking doughs brings important technological and nutritional challenges, and the use of the sourdough biotechnology has the potential to overcome such limitations. In addition, the typical metabolic transformations carried out by Lactic Acid Bacteria (LAB) can become an important biotechnological process for the nutritional fortification and functionalization of sourdoughs due to the resulting postbiotics. In such a context, this research work aimed at isolating and selecting new LAB strains that resort to a wide range of natural environments and food matrices to be ultimately employed as starter cultures in gluten-free sourdough fermentations. Nineteen LAB strains belonging to the genera of Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus were isolated, and the selection criteria encompassed their acidification capacity in fermentations carried out on chickpea, quinoa, and buckwheat flour extracts; the capacity to produce exopolysaccharides (EPS); and the antimicrobial activity against food spoilage molds and bacteria. Moreover, the stability of the LAB metabolites after the fermentation of the gluten-free flour extracts submitted to thermal and acidic treatments was also assessed.

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Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

Cited by 66 publications

References 43 publications

Exploration of an Extracellular Polymeric Substance from Earthworm Gut Bacterium (Bacillus licheniformis) for Bioflocculation and Heavy Metal Removal Potential

Exploration of an Extracellular Polymeric Substance from Earthworm Gut Bacterium (Bacillus licheniformis) for Bioflocculation and Heavy Metal Removal Potential

Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

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