Characterization of an endonuclease activity which preferentially cleaves the G-rich immunoglobulin switch repeat sequences

Lyon, Christopher J.; Miranda, Gustavo A.; Piao, Jin S.; Aguilera, Renato J.

doi:10.1016/0161-5890(95)00125-5

Cited by 17 publications

(10 citation statements)

References 58 publications

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“…In an effort to identify a novel nuclease activity involved in immunoglobulin isotype switch recombination, work from our laboratory identified Endo-SR, which preferentially cleaved DNA within switch recombination sequences (Lyon et al, 1996;Lyon and Aguilera, 1997). Furthermore, Endo-SR exhibited an acid pH optimum and functioned independently of divalent cations.…”

Section: Identification and Characterization Of The Mammalian Dnase Imentioning

confidence: 98%

“…Furthermore, Endo-SR exhibited an acid pH optimum and functioned independently of divalent cations. Endo-SR was subsequently purified to homogeneity from bovine spleen and subjected to peptide microsequencing (Lyon et al, 1996;Lyon, 1997;Lyon and Aguilera, 1997). Three separate peptide sequences were obtained from this analysis and compared to database sequences.…”

Section: Identification and Characterization Of The Mammalian Dnase Imentioning

confidence: 99%

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DNase II: genes, enzymes and function

Evans

Aguilera

2003

Gene

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213

155

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Section: Identification and Characterization Of The Mammalian Dnase Imentioning

confidence: 98%

Section: Identification and Characterization Of The Mammalian Dnase Imentioning

confidence: 99%

DNase II: genes, enzymes and function

Evans

Aguilera

2003

Gene

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213

155

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“…Consistent with a generalized role in DNA degradation and an acidic pH optima, DNase II enzymes exhibit a ubiquitous tissue distribution and are localized within lysosomes [1,3]. While DNase II enzymes can degrade DNA substrates to completion, they also exhibit cleavage preferences that are site-and speciesspecific [1,[4][5][6].…”

Section: Introductionmentioning

confidence: 95%

DNase II deficiency impairs innate immune function in Drosophila

Seong

Varela-Ramı́rez

Aguilera

2006

Cellular Immunology

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DNase II enzymes are highly conserved proteins that are required for the degradation of DNA within phagolysosomes. Engulfment of apoptotic cells and/or bacteria by phagocytic cells requires the function of DNase II to completely destroy ingested DNA. Mutation of the dnase II gene results in an increase of undegraded apoptotic DNA within phagocytic cells in mice and nematodes. Additionally, reduction of DNase II enzymatic activity in Drosophila melanogaster has been shown to lead to increased accumulation of DNA in the ovaries. Due to the importance of DNA clearance during infection, we hypothesized that a severe reduction of DNase II activity would result in diminished immune function and viability. To test this hypothesis, we knocked down DNase II activity in flies using RNAi. As expected, expression of a dnase II-RNAi construct in flies resulted in a dramatic reduction of DNase II activity and a significant decrease in total hemocyte numbers. Furthermore, infection of dnase II-RNAi flies with Gram negative or positive bacteria resulted in a severe reduction in fly viability. These results confirm that DNase II and the ability to clear macromolecular DNA is essential for maintaining proper immune function in Drosophila.

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“…By using a biochemical approach, Lion et al (43,44) isolated and purified a protein with endonuclease activity (Endo-SR) from murine and bovine spleen that could specifically recognize and cleave GC-rich switch region DNA sequences. The human analog of Endo-SR has not been described and the cloning of the gene encoding Endo-SR has not yet been reported.…”

Section: Other Candidate Proteins In Csrmentioning

confidence: 99%

Immunoglobulin Class Switch Recombination Machinery: Progress and Challenges

Zhang¹

2000

Clinical Immunology

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Characterization of an endonuclease activity which preferentially cleaves the G-rich immunoglobulin switch repeat sequences

Cited by 17 publications

References 58 publications

DNase II: genes, enzymes and function

DNase II: genes, enzymes and function

DNase II deficiency impairs innate immune function in Drosophila

Immunoglobulin Class Switch Recombination Machinery: Progress and Challenges

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