Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag- and CMA&lt;sub&gt;3&lt;/sub&gt;-staining, and fluorescent in situ hybridization

Castro, Jaime; Viñas, Ana; Sánchez, Laura; Martı́nez, Paulino

doi:10.1159/000134491

Cited by 45 publications

(54 citation statements)

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“…1, 2 and 4). This is in accordance with numerous reports about the findings of polymorphism of NOR in Moenkhausia sanctaefilomenae (Foresti et al 1989), Aphanius fasciatus (Vitturi et al 1995), Leporinus friderici (Galetti et al 1995), Salmo trutta (Castro et al 1996), Salvelinus alpines (Reed and Phillips 1997), Chondrostoma lusitanicum (Rodrigues andCollaresPereira 1996, Collares-Pereira andRáb 1999), Hoplias malabaricus (Born and Bertollo 2000), Oedalechilus labeo (Rossi et al 2000), Astyanax scabripinnis (Mantovani et al 2000, Marco-Ferro et al 2001, Soza et al 2001, A. altiparanae (Pacheco et al 2001, Mantovani et al 2005 (Vitturi et al 2005), Prochilodus lineatus (Gras et al 2007), and B. aff. iheringii (Capistano et al 2008).…”

Section: Resultssupporting

confidence: 93%

A Discovery of Nucleolar Organizer Regions (NORs) Polymorphism and Karyological Analysis of Smith's Barb, <i>Puntioplites proctozysron</i> (Cypriniformes, Cyprinidae) in Thailand

et al. 2012

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Summary A discovery of nucleolar organizer regions (NORs) polymorphism and karyological analysis in the Smith s barb, Puntioplites proctozysron (Bleeker, 1865) from Nong Khai province, in the northeast of Thailand, were studied. Blood samples were taken from 4 male and 4 female fish. After standard whole blood lymphocytes had been cultured at 27 C for 96 hours in the presence of colchicine, metaphase spreads were performed on microscopic slides and air-dried. Conventional and Ag-NOR staining techniques were applied to stain the chromosome. The results showed that the diploid chromosomes number of P. proctozysron was 2n=50 and the fundamental numbers (NF) of both sexes were 82. The types of autosomes were 4 large metacentric, 8 large submetacentric, 2 large acrocentric, 2 large telocentric, 2 medium metacentric, 6 medium submetacentric, 10 medium acrocentric, 14 medium telocentric and 2 small telocentric chromosomes. No strange size chromosomes related to sex were observed. In addition, the short arm near telomere of chromosome pair 4 showed clearly observable nucleolar organizer regions (NORs). This is the first report on polymorphism of NORs in P. proctozysron. The result showed that a heteromorphism in 1 female had a different size of NORs of chromosome pair 4 (4a4b), while 3 females and 4 males had an equal size of both chromosome pair 4 with a homomorphism (4a4a). The karyotype formula for P. proctozysron is as follows: 2n (diploid) 50=L

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Section: Resultssupporting

confidence: 93%

A Discovery of Nucleolar Organizer Regions (NORs) Polymorphism and Karyological Analysis of Smith's Barb, <i>Puntioplites proctozysron</i> (Cypriniformes, Cyprinidae) in Thailand

et al. 2012

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“…Coupled with chromosomal rearrangements, transposition events have also been considered responsible for the NOR dispersal in genomes of different groups (Castro et al 1996, Almeida-Toledo et al 1996. So far, both 5S or 18S ribosomal genes have never been identified on the B-microchromosomes of P. lineatus (Jesus and Moreira-Filho 2003), as also shown in the present data.…”

Section: Discussionsupporting

confidence: 71%

Cytogenetic Markers in Wild Population of Curimbata (Prochilodus lineatus) from Mogi-Guacu River

et al. 2009

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Summary Within the genus Prochilodus, the species Prochilodus lineatus is certainly the most studied one from a cytogenetic point of view. In this study, a cytogenetic characterization of specimens of P. lineatus from Mogi-Guaçu River was performed in the period from 2003 to 2007, through utilization of cytogenetic markers, such as Giemsa, Ag-NOR, C-banding and cytogeneticalmolecular markers (FISH) to detect both 5S and 18S ribosomal genes. All analyzed individuals presented 2nϭ54 meta/submetacentric chromosomes, besides bearing up to 7 supernumerary microchromosomes. Polymorphic NORs were detected on a single chromosomal pair. The constitutive heterochromatin was distributed at centromeric region of all chromosomes in the A complement, while the microchromosomes were totally heterochromatic. A syntenic distribution of 5S and 18S ribosomal genes was detected, comprising the NOR-bearing chromosomal pair. No additional ribosomal clusters in other chromosomes were observed. Although the karyotype features are commonly conserved within the genus Prochilodus, the few differences on the distribution of both ribosomal genes and heterochromatin when compared to specimens of P. lineatus from other localities can be associated to the evolutionary changes that these repetitive sequences have undergone through the years.

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“…Heteromorphic NORs detected in P. squamosissimus suggest that such phenotypes may be valuable for genetical recognition of different populations in the central Amazon. Variations in copy number and site distribution of rDNA clusters/NORs have been described extensively in fish species, and have allowed the characterization of intraspecific stocks or populations in some fish groups (Phyllips et al, 1988;Castro et al, 1996), but to our knowledge the present study is the first to show this situation in Neotropical Perciformes.…”

Section: Discussionmentioning

confidence: 60%

Cytogenetic studies of two freshwater sciaenids of the genus Plagioscion (Perciformes, Sciaenidae) from the central Amazon

et al. 1999

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Cytogenetic characterization of two freshwater sciaenid species from the genus Plagioscion (P. squamosissimus and Plagioscion sp.) was obtained for the first time. Giemsa staining, Ag-NOR and C-banding revealed that both species presented 2n = 48 chromosomes (almost all acrocentric). Single NORs and heterochromatin were found mainly at the pericentromeric position. Karyotypic formulae and NOR location proved to be valuable in showing both interspecific and intraspecific differences. All chromosomes were acrocentric in P. squamosissimus. NORs were located at proximal positions on the long arms of the last chromosome pair of the complement, and were heteromorphic due to size differences. Such heteromorphic NORs seem to be associated with each population sampled. Plagioscion sp. presented two cytotypes: cytotype a (2M + 46A) and cytotype b (48A). In both cytotypes, NOR-bearing chromosomes were located at the proximal position on the long arms of the first chromosome pair of the complement. However, NOR-bearing chromosomes were metacentric in cytotype a and acrocentric in cytotype b.

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Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag- and CMA<sub>3</sub>-staining, and fluorescent in situ hybridization

Cited by 45 publications

References 10 publications

A Discovery of Nucleolar Organizer Regions (NORs) Polymorphism and Karyological Analysis of Smith's Barb, <i>Puntioplites proctozysron</i> (Cypriniformes, Cyprinidae) in Thailand

A Discovery of Nucleolar Organizer Regions (NORs) Polymorphism and Karyological Analysis of Smith's Barb, <i>Puntioplites proctozysron</i> (Cypriniformes, Cyprinidae) in Thailand

Cytogenetic Markers in Wild Population of Curimbata (Prochilodus lineatus) from Mogi-Guacu River

Cytogenetic studies of two freshwater sciaenids of the genus Plagioscion (Perciformes, Sciaenidae) from the central Amazon

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