Characterization of an aryl hydrocarbon receptor from a cetacean : an approach for assessing contaminant susceptibility in protected species

Jensen, Brenda A.

doi:10.1575/1912/4071

Cited by 5 publications

(8 citation statements)

References 139 publications

(173 reference statements)

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“…The absence of a band around 429 bp indicated that CeMV was not detected. Amplification of a 322 bp fragment of the housekeeping gene β-actin was used as a positive control for RNA extraction and reverse transcription (Table 1) (Jensen 2000). The positive PCR control consisted of template cDNA from one of several tissues from the Longman's beaked whale that was confirmed positive by the Athens Diagnostic Laboratory (College of Veterinary Medicine, University of Georgia, Athens, GA) (West et al 2013).…”

Section: Characterization Of Cemv Isolates Using P-and N-genesmentioning

confidence: 99%

Initial characterization of novel beaked whale morbillivirus in Hawaiian cetaceans

Jacob

West²,

Levine³

et al. 2016

Dis. Aquat. Org.

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Cetacean morbillivirus (CeMV) is a causative factor in epizootics that have resulted in thousands of deaths throughout the Atlantic and Mediterranean since 1987, but less is known of its presence and significance in the Pacific. The first case of CeMV reported in Hawai'i was in a Longman's beaked whale that stranded in 2010. The initial CeMV sequence from this individual indicated the possibility of a novel strain. To address this, archived samples from cetaceans that stranded in Hawai'i between 1997 and 2014 were screened for CeMV. The beaked whale morbillivirus (BWMV) was detected in 15 individuals representing 12 different species (24% of Code 1 and 2 stranded cetaceans). The earliest detected case was a humpback whale that stranded in 1998. Sequence comparisons of a 2.2 kb sequence spanning the phosphoprotein (P) and nucleocapsid (N) genes strongly suggest that the BWMV represents a novel strain of CeMV present in Hawai'i and the Central Pacific. In contrast to recently reported isolates from Brazil and Australia that may represent a distinct clade, BWMV appears to be more closely related to known strains of CeMV (dolphin morbillivirus; porpoise morbillivirus; and pilot whale morbillivirus). Detection rates with repeat sampling of positive lymph nodes were between 2 and 61%, illustrating the extreme heterogeneity that can occur in affected tissues. Taken together, these results suggest that BWMV may be common and established in Hawaiian cetacean populations. BWMV will be important for understanding CeMV and health threats in the relatively understudied cetaceans of the Pacific.

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Section: Characterization Of Cemv Isolates Using P-and N-genesmentioning

confidence: 99%

Initial characterization of novel beaked whale morbillivirus in Hawaiian cetaceans

Jacob

West²,

Levine³

et al. 2016

Dis. Aquat. Org.

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“…Despite several experiments under various conditions, binding of [ 3 H]TCDD to the human AHR was not sufficiently stable in this assay to allow determination of [ 3 H]TCDD SB or its displacement by competitors (Jensen 2000). This is consistent with a previous report that the HAP assay was not effective in measuring [ 3 H]TCDD binding to AHR from human placenta (Nakai and Bunce 1995).…”

Section: Resultsmentioning

confidence: 99%

“…For compounds that did not compete as well for [ 3 H]TCDD binding (e.g. 105, 118) the fitted curve minimum was far from 0%, and the IC 50 s calculated from the “fixed” fits tended to be higher than those estimated from the “unfixed” fits (Jensen 2000). In order to best compare the binding of HAH compounds, the data from the “fixed” analysis were used throughout.…”

Section: Methodsmentioning

confidence: 99%

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Developing tools for risk assessment in protected species: Relative potencies inferred from competitive binding of halogenated aromatic hydrocarbons to aryl hydrocarbon receptors from beluga (Delphinapterus leucas) and mouse

et al. 2010

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Persistent organic pollutants such as halogenated aromatic hydrocarbons (HAHs) biomagnify in food webs and accumulate to high concentrations in top predators like odontocete cetaceans (toothed whales). The most toxic HAHs are the 2,3,7,8-substituted halogenated dibenzo-p-dioxins and furans, and non-ortho-substituted polychlorinated biphenyls (PCBs), which exert their effects via the aryl hydrocarbon receptor (AHR). Understanding the impact of HAHs in wildlife is limited by the lack of taxon-specific information about the relative potencies of toxicologically important congeners. To assess whether Toxic Equivalency Factors (TEFs) determined in rodents are predictive of HAH relative potencies in a cetacean, we used beluga and mouse AHRs expressed in vitro from cloned cDNAs to measure the relative AHR-binding affinities of ten HAHs from five different structural classes. The rank order of mean IC50s for competitive binding to beluga AHR was: TCDD

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“…AHR homologues have been identified in vertebrates including mammals, birds, amphibians, bony fish, cartilaginous fish and jawless fish [31][32][33][34][35][36][37][38]. Various analytic efforts including ligand-binding assays, antibody cross-reactivity and photoaffinity labeling have been used in attempts to identify an invertebrate AHR homologue [39][40].…”

Section: Innate Immunity Xenobiotics and Signalingmentioning

confidence: 99%

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et al. 2006

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Characterization of an aryl hydrocarbon receptor from a cetacean : an approach for assessing contaminant susceptibility in protected species

Cited by 5 publications

References 139 publications

Initial characterization of novel beaked whale morbillivirus in Hawaiian cetaceans

Initial characterization of novel beaked whale morbillivirus in Hawaiian cetaceans

Developing tools for risk assessment in protected species: Relative potencies inferred from competitive binding of halogenated aromatic hydrocarbons to aryl hydrocarbon receptors from beluga (Delphinapterus leucas) and mouse

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