Characterization of an Antibiotic Produced by Bacillus subtilis JW-1 that Suppresses Ralstonia solanacearum

Kwon, Jae Won; Kim, Shin Duk

doi:10.4014/jmb.1308.08060

Cited by 22 publications

(23 citation statements)

References 16 publications

(18 reference statements)

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“…These results are contrariwise those of Kheirandish and Harighi (2015) who found that different strains of P. fluorescens antagonized the growth of R. solanacerum. Also, these results are on the contrary of those of Kwon and Kim (2014) who demonstrated that B. subtilis suppressed the growth of R. solanacerum. In addition, Nath et al (2016) had counteractive results of this investigation where they showed that B. subtilis and P. fluorescens inhibited the growth of R. solanacearum.…”

Section: Inhibition Effects and Micscontrasting

confidence: 94%

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

Ezzat

Moussa

2016

Journal of Productivity and Development

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Brown rot of potato is a worldwide disease that causes huge agricultural and economical losses. Using synthetic chemicals like antimicrobial pesticide may have adverse effects on consumers and on the environment. The recent trend of control plant disease is the application of eco-friendly tools to control disease. In the present investigation, and this study streptomycin, Pleurotus columbinus spent wheat straw (PCSWS), wheat straw (WS), three bacterial species (Bacillus subtillus, B. polymyxa and Pseudomonas fluorescens), plant essential oils of anethole, mustard, caraway and neem were tested to control brown rot disease of potato cv. Spunta in Baramoon Research Station, Dakahlia Governorate, Egypt under field conditions, during the two winter seasons of 2013/14 and 2014/15. The vegetative growth parameters, tuber yield, tuber grading, tuber dry matter, specific gravity and infected tubers were investigated. PCSWS led to significant increase in all studied criteria, compared to check or other treatments. Furthermore, PCSWS or caraway oil gave the best results in reduction of infected tubers in vitro. The direct antibacterial effect of studied treatments against the causal pathogen of brown rot of potato; Ralstonia solanacerum. Only streptomycin, anethole and caraway oil affected the growth of R. solanacearum. Pots experiment was carried out to study the ability of the tested treatments to induce systemic resistance (ISR).Conclusively, Bacillus subtillus, caraway and neem oils significantly increased the production of total phenols, polyphenol oxidase and peroxidase, respectively, which enhanced potato plant health, increased chlorophylls and plant height as well as, decreased disease rating that increased tuber weight after 70 days of planting. Preparation of bacterial pathogen and bacterial bio-agents:Bacterial pathogen (R. solanacearum) and PGPR bio-agents of B. subtilis, B. polymyxa and P. fluorescens were inoculated in nutrient growth Glucose Broth (NGB) and incubated in shaker incubator (28 °C and 120 RPM) for 48 hrs. Bacteria were harvested by centrifugation (10000 rpm/ min for 20 min.), each bacterial species was re-suspend in sterilized tap water and each bacterial suspension was adjusted to 10 8 cfu / ml concentration.R. solanacearum bacterial suspension was used for moist soil in pot experiment before 48 hrs of planting. Bacterial bio-agents' suspensions were used to soaking potato seeds for 30 min. before planting in the field and pot experiments.For preparation the bacterial suspension to in vitro experiments, studied bacteria were seeded on nutrient glucose agar (NGA) slants for 48 hrs, harvested, re-suspended in sterilized tap water and adjusted to 10 8 cfu / ml concentration. Organic wastesPleurotus columbinus spent wheat straw (PCSWS) was provided from Mas Mushroom Co., Talkha, Dakahlia governorate, Egypt. Only, wheat straw (WS) powder was prepard from the same Mushroom Co. that used for P. columbinus cultivation. PCSWS and WS were grinded well to fine powder. PCSWS and WS were used for coating ...

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Section: Inhibition Effects and Micscontrasting

confidence: 94%

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

Ezzat

Moussa

2016

Journal of Productivity and Development

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“…Many studies have shown that the use of Bacillus strains as biological control agents is as a promising and safe strategy for effective tomato bacterial wilt management. Kwon and Kim reported that B. subtilis JW-1 could lead to a >80% reduction in bacterial wilt disease and can be used as a potential biocontrol agent of tomato bacterial wilt [42]. Xiong et al isolated the B. amyloliquefaciens JK6 strain and found that it could effectively suppress tomato bacterial wilt with biocontrol efficacies for up to 52.9% under two greenhouse conditions [39].…”

Section: Discussionmentioning

confidence: 99%

“…Bais et al reported that B. subtilis 6051 was able to control Pseudomonas syringae root infection in Arabidopsis because of the secretion of surfactin and the formation of a biofilm on the plant roots [15]. Kwon and Kim demonstrated that B. subtilis JW-1 induced a significant bacterial wilt disease suppression effect in vivo due to the production of cyclic lipopeptides [42]. Xiong et al reported that the secretion of surfactin by the strain JK6 played important roles in the biocontrol of tomato bacterial wilt [39].…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

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Background: There is an urgent need to discover biological biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results: Based on a whole genome sequence analysis, the new strain FJAT-46737 was identified as Bacillus velezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (including Ralstonia solanacearum, Escherichia coli and Fusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides against R. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20 oC to 40 oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20 oC and 25oC. Conclusions: The B. velezensis strain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogen R. solanacearum.

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Section: Velezensismentioning

confidence: 99%

“…Bais et al reported that B. subtilis 6051 was able to control Pseudomonas syringae root infection in Arabidopsis because of the secretion of surfactin and the formation of a biofilm on the plant roots [15]. Kwon and Kim demonstrated that B. subtilis JW-1 induced a significant bacterial wilt disease suppression effect in vivo due to the production of cyclic lipopeptides [41]. Xiong et al reported that the secretion of surfactin by the strain JK6 played important roles in the biocontrol of tomato bacterial wilt [38].…”

Section: Velezensismentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: There is an urgent need to discover alternative Bacillus biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results: Based on a whole genome sequence analysis, the new strain FJAT-46737 was identified as Bacillus velezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (including Ralstonia solanacearum, Escherichia coli and Fusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides against R. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20 oC to 40 oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20 oC and 25oC. Conclusions: The B. velezensis strain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogen R. solanacearum.

show abstract

Characterization of an Antibiotic Produced by Bacillus subtilis JW-1 that Suppresses Ralstonia solanacearum

Cited by 22 publications

References 16 publications

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

INVESTIGATING THE EFFECT OF SOME ELICITORS ON BROWN ROT DISEASE AND TUBER YIELD OF POTATO (Solanum tuberosum L.)

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

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