Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus

Abstract

We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus ostreatus fruiting body was treated with distilled water at 40℃ for 48 hr. The XOD inhibitor thus obtained was purified by Sephadex G-50 gel permeation chromatography, ultrafiltration, C18 solid phase extraction chromatography and reverse-phase high-performance liquid chromatography with 3% of solid yield, and its XOD inhibitory activity was 0.9 mg/mL of IC50. The purified XOD inhibitor was a tripeptide with the amino acid sequence phenylalanine-cysteine-histidine and a molecular weight of 441.3 Da. The XOD inhibitor-containing ultrafiltrates from Pleurotus ostreatus demonstrated dose-dependent anti-gout effects in a Sprague-Dawley rat model of potassium oxonate-induced gout, as shown by decreased serum urated levels at doses of 500 and 1,000 mg/kg, although the effect was not as great as that achieved with the commercial anti-gout agent, allopurinol when administered at a dose of 50 mg/kg.

Hypouricemic Effect of Ethanol Extract of Aster glehni Leaves in Potassium Oxonate-Induced Hyperuricemic Rats

“…A PO-induced hyperuricemic rat model was used to evaluate attenuation effect of EAG [ 16 17 ]. Thirty-six rats were randomly divided into 6 groups.…”

Crystal structure of (E)-3-(2-hydroxy-4-methylphenyl)-1-(2,4,6-trimethoxyphenyl)prop-2-en-1-one

“…Structurally, they consist of two aryl groups linked by an , -unsaturated ketone system (Ibrahim et al, 2012;Kumar et al, 2013), whereby the aryl groups can carry a variety of substituents such as hydroxyl, methoxy and alkenyl groups, which are by far the most commonly encountered ones in nature. With their structural simplicity and the associated ease of synthesis, chalcone compounds have attracted a considerable amount of attention because of their important pharmacological properties such as antioxidative (Aoki et al, 2008), anti-inflammatory (Israf et al, 2007), anti-gout (Jang et al, 2014), anti-histaminic (Yamamoto et al, 2004), anti-obesity (Birari et al, 2011), anti-protozoal (Chen et al, 1993), hypnotic (Cho et al, 2011) and anti-spasmodic (Sato et al, 2007) effects. In a continuation of our ongoing research on the properties of various chalcone derivatives (Sim et al, 2017, Kwong et al, 2018, we report herein the synthesis and crystal structure determination of the title compound, C 19 H 20 O 5 , (I).…”

Exploration of Microorganisms as a Potential Source of Xanthine Oxidase Inhibitors: An Updated Review

“…The acetonic, methanolic and hot water extracts from the fruiting bodies of Pleurotus salmoneoshamineus and P. nebrodensis were reported to have XO inhibition which was found to be increased with increasing concentrations [55,56]. In a similar way, a new compound was purified from the aqueous extract of Pleurotus ostreaus and has exhibited XO inhibitory activity with an IC50 value of 0.9 mg/ml [57].…”