Characterization of an anther- and tapetum-specific gene and its highly specific promoter isolated from tomato

Abundant Type III Lipid Transfer Proteins in Arabidopsis Tapetum Are Secreted to the Locule and Become a Constituent of the Pollen Exine

“…The localization of LTP transcripts in anthers has been extensively reported, in part because anthers are essential for producing pollen and contain abundant metabolically active and secretory tapetum cells to which the transcripts could locate. In situ hybridization or LTP-promoter-GUS transformation has localized LTP transcripts or proteins in anthers and/or the tapetum of tomato (Solanum lycopersicum; TomA108 [Chen et al, 2006] and TomA5B [Aguirre and Smith, 1993]), tobacco (Nicotiana tabacum; TA32 and TA36 [Koltunow et al, 1990]), Brassica species and Arabidopsis (Arabidopsis thaliana; A9 [Paul et al, 1992;Turgut et al, 1994]), rice (Oryza sativa; YY1 [Tsuchiya et al, 1994], Osc4 [Hihara et al, 1996], and several other genes [Huang et al, 2009;Zhang et al, 2010]), sugar beet (Beta vulgaris; BvLTP1 and BvLTP2 [Matsuhira et al, 2006]), lily (Lilium longiflorum; LHM6 and LHM7 [Crossley et al, 1995]), and maize (Zea mays; MZm3-3 [Lauga et al, 2000]). However, because of the low sensitivity of detection methods, especially those used in earlier years, whether LTP transcripts are also present in lesser amounts in other organs/tissues/cells, such as the anther epidermis, is unknown.…”