Characterization of an 17β-estradiol-degrading bacterium Stenotrophomonas maltophilia SJTL3 tolerant to adverse environmental factors

Xiong, Weiliang; Chen, Yin; Peng, Wen; Deng, Zixin; Lin, Shuangjun; Liang, Rubing

doi:10.1007/s00253-019-10281-8

Cited by 16 publications

(22 citation statements)

References 45 publications

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“…ES2-1 was expressed in the presence of E2 (Whirl-Carrillo et al, 2012;Hsiao et al, 2020;Ibero et al, 2020;Li et al, 2021). However, gene expression or gene knockout experiments are required to provide conclusive Hu et al, 2011;Liang et al, 2012;Zhang Y. et al, 2012;Ye et al, 2017;Xiong et al, 2018Xiong et al, , 2020Zhao et al, 2018;Qiu et al, 2019;Ibero et al, 2020;Pratush et al, 2020;Tian et al, 2020; Zhao et al, 2018;Hsiao et al, 2020;Ibero et al, 2020;Tian et al, 2020;Xiong et al, 2020;Li et al, 2021 evidence that these genes are responsible for the metabolism of E2 by R. equi ATCC13557.…”

Section: Discussionmentioning

confidence: 99%

“…Genomic and transcriptomic studies have revealed numerous other genes putatively involved in oestrogen and steroid metabolism. The genome of Stenotrophomonas maltophilia SJTL3 contains 12 short-chain dehydrogenase/reductase (SDR) encoding genes, the superfamily of genes to which 17β-hydroxysteroid dehydrogenase belongs ( Xiong et al, 2020 ). It also contains two 3β-hydroxysteroid dehydrogenases, five dioxygenase-encoding genes, two FAD-dependent monooxygenase-encoding genes, and 17 other dehydrogenases including acyl-CoA dehydrogenase and alcohol dehydrogenase putatively involved in the downstream degradation of oestrogens ( Xiong et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The genome of Stenotrophomonas maltophilia SJTL3 contains 12 short-chain dehydrogenase/reductase (SDR) encoding genes, the superfamily of genes to which 17β-hydroxysteroid dehydrogenase belongs ( Xiong et al, 2020 ). It also contains two 3β-hydroxysteroid dehydrogenases, five dioxygenase-encoding genes, two FAD-dependent monooxygenase-encoding genes, and 17 other dehydrogenases including acyl-CoA dehydrogenase and alcohol dehydrogenase putatively involved in the downstream degradation of oestrogens ( Xiong et al, 2020 ). Four of the genes encoding acyl-CoA dehydrogenase within the genome of S. maltophilia SJTL3 showed high similarity, containing the same Y-X-X-D/E-R motif, to a 17β-hydroxysteroid dehydrogenase identified within the genome of Sphingomonas strain KC8 ( Chen et al, 2017 ; Xiong et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…It also contains two 3β-hydroxysteroid dehydrogenases, five dioxygenase-encoding genes, two FAD-dependent monooxygenase-encoding genes, and 17 other dehydrogenases including acyl-CoA dehydrogenase and alcohol dehydrogenase putatively involved in the downstream degradation of oestrogens ( Xiong et al, 2020 ). Four of the genes encoding acyl-CoA dehydrogenase within the genome of S. maltophilia SJTL3 showed high similarity, containing the same Y-X-X-D/E-R motif, to a 17β-hydroxysteroid dehydrogenase identified within the genome of Sphingomonas strain KC8 ( Chen et al, 2017 ; Xiong et al, 2020 ). The genes coding for the putative enzymes catechol 1,2 dioxygenase, dioxygenase, and 7α-hydroxysteroid dehydrogenase were expressed in Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

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Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557

et al. 2021

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Rhodococcus equi ATCC13557 was selected as a model organism to study oestrogen degradation based on its previous ability to degrade 17α-ethinylestradiol (EE2). Biodegradation experiments revealed that R. equi ATCC13557 was unable to metabolise EE2. However, it was able to metabolise E2 with the major metabolite being E1 with no further degradation of E1. However, the conversion of E2 into E1 was incomplete, with 11.2 and 50.6% of E2 degraded in mixed (E1-E2-EE2) and E2-only conditions, respectively. Therefore, the metabolic pathway of E2 degradation by R. equi ATCC13557 may have two possible pathways. The genome of R. equi ATCC13557 was sequenced, assembled, and mapped for the first time. The genome analysis allowed the identification of genes possibly responsible for the observed biodegradation characteristics of R. equi ATCC13557. Several genes within R. equi ATCC13557 are similar, but not identical in sequence, to those identified within the genomes of other oestrogen degrading bacteria, including Pseudomonas putida strain SJTE-1 and Sphingomonas strain KC8. Homologous gene sequences coding for enzymes potentially involved in oestrogen degradation, most commonly a cytochrome P450 monooxygenase (oecB), extradiol dioxygenase (oecC), and 17β-hydroxysteroid dehydrogenase (oecA), were identified within the genome of R. equi ATCC13557. These searches also revealed a gene cluster potentially coding for enzymes involved in steroid/oestrogen degradation; 3-carboxyethylcatechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde hydrolase, 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase, 3-(3-hydroxy-phenyl)propionate hydroxylase, cytochrome P450 monooxygenase, and 3-oxosteroid 1-dehydrogenase. Further, the searches revealed steroid hormone metabolism gene clusters from the 9, 10-seco pathway, therefore R. equi ATCC13557 also has the potential to metabolise other steroid hormones such as cholesterol.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557

et al. 2021

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“…Bacteria that degrade these hormones belong to genera such as Bacillus , Virgibacillus , Novosphingobium , Rhodococcus , Acinetobacter , Pseudomonas , Sphingomonas , Enterobacter , Klebsiella , Aeromonas , Comamonas , Thauera , Deinococcus , Stenotrophomonas and cyanobacteria [ 3 , 4 , 6 , 7 , 10 , 12 , 25 , 28 , 31 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. Raphidocelis subcapitata , Chlorella vulgaris , Chlorococcus and Scenedesmus quadricauda belong to algae capable of removing hormones [ 11 , 26 , 40 , 41 ].…”

Section: Microbiological Degradation Of Edcsmentioning

confidence: 99%

Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds

Wojcieszyńska

Marchlewicz

2020

Molecules

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The rising pollution of the environment with endocrine disrupting compounds has increased interest in searching for new, effective bioremediation methods. Particular attention is paid to the search for microorganisms with high degradation potential and the possibility of their use in the degradation of endocrine disrupting compounds. Increasingly, immobilized microorganisms or enzymes are used in biodegradation systems. This review presents the main sources of endocrine disrupting compounds and identifies the risks associated with their presence in the environment. The main pathways of degradation of these compounds by microorganisms are also presented. The last part is devoted to an overview of the immobilization methods used for the purposes of enabling the use of biocatalysts in environmental bioremediation.

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Opportunities and Challenges for Sustainable Bioremediation of Natural and Synthetic Estrogens as Emerging Water Contaminants Using Bacteria, Fungi, and Algae

Ratnasari

Syafiuddin

Kueh

et al. 2021

Water Air Soil Pollut

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Characterization of an 17β-estradiol-degrading bacterium Stenotrophomonas maltophilia SJTL3 tolerant to adverse environmental factors

Cited by 16 publications

References 45 publications

Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557

Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557

Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds

Opportunities and Challenges for Sustainable Bioremediation of Natural and Synthetic Estrogens as Emerging Water Contaminants Using Bacteria, Fungi, and Algae

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