Characterization of allergens from Trisetum paniceum pollen: an important aeroallergen in Mediterranean continental climatic areas

Montero, M. Teresa; López, Carmen; Jiménez, J A; Subiza, José Luis

doi:10.1111/j.1365-2222.1997.tb02989.x

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…One hundred percent of grass-allergic patients are sensitised to this grass species [6]. T. paniceum pollen has a high proportion of IgE-reactive proteins, including an atypical proportion of basic components which are not detected in other phylogenetically related pollens [7]. The latter study demonstrated cross-reactivity between T. paniceum and other grass pollens, such as Lolium perenne, Festuca elatior, Poa pratensis, Dactylis glomerata, Phleum pratense and Holcus lanatus.…”

Section: Introductionmentioning

confidence: 98%

<i>Trisetum paniceum</i> (Wild Oats) Pollen Counts and Aeroallergens in the Ambient Air of Madrid, Spain

Cabrera¹,

Martínez-Cócera²,

Fernández-Caldas³

et al. 2002

Int Arch Allergy Immunol

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Background: Madrid has a short but intensive grass pollen season, in which 79% of the total grass pollen load is released from the middle of May to the middle of June. The main objectives of this study were to quantify Trisetum paniceum (wild oats) aeroallergen in the atmosphere in Madrid from February to December 1996 and to correlate the aeroallergen concentrations with grass pollen counts. Methods: Two different samplers were used to assess allergen exposure; a Burkard spore trap was used to collect pollen grains and a high-volume air sampler to collect airborne particles. A total of 182 air filters were collected and extracted in 1 ml of PBS and analysed by ELISA inhibition, using pooled sera from highly allergic individuals. Results:T. paniceum aeroallergens were detected not only during the grass pollen season, but also before and after. Wild oat allergens had two main peaks of 1 and 1.9 µg/m³, occurring in late May and July, respectively. The time series analysis established the existence of lags between the two main variables pollen counts and aeroallergen activity. Analysis of the data by the Spearman rank test and linear regression showed a weak correlation between grass allergenic activity and grass pollen counts (Spearman’s rho = 0.29). Data obtained from time series analysis demonstrated that grass allergenic activity correlated strongly with current and 5-week-old grass pollen grain counts (r² = 0.73). Conclusions: Wild oats allergenic activity was detected during the entire year and not only during the pollen season. This fact is an important aspect to be considered in the clinical follow-up and treatment of grass pollen-sensitised patients in Madrid.

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Section: Introductionmentioning

confidence: 98%

<i>Trisetum paniceum</i> (Wild Oats) Pollen Counts and Aeroallergens in the Ambient Air of Madrid, Spain

Cabrera¹,

Martínez-Cócera²,

Fernández-Caldas³

et al. 2002

Int Arch Allergy Immunol

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“…D. glomerata also belongs to the Poaceae family and is widely distributed in Spain and most European countries. While the allergenicity of D. glomerata is well established [24], less is known about the allergenicity of T. paniceum, but it also seems to contain Groups 1 and 5 allergens [25].…”

Section: Introductionmentioning

confidence: 99%

Cluster immunotherapy with a glutaraldehyde‐modified mixture of grasses results in an improvement in specific nasal provocation tests in less than 2.5 months of treatment

Subiza

Felíu

Subiza

et al. 2008

Clin Experimental Allergy

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“…Japanese scientists currently use agarose IEF for standard proteomics investigations such as the differential analysis of cancer versus control samples (esophagus 113, liver 114, pancreas 115, 116, colon‐rectum 117, prostate 118), the search for biomarkers (alcoholism 119, X‐ray resistance 120) or the identification of allergens ( Trisetum paniceum pollen 121). However the most specific fields of application of agarose IEF may be in the analysis of high M r components and of membrane proteins.…”

Section: Analysis Of High Molecular Size Proteinsmentioning

confidence: 99%

Other than IPG‐DALT: 2‐DE variants

2010

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Special problems require special solutions. That is why, despite the trend towards standardization of the procedures in proteomic investigations, a number of alternative protocols, based on 2-DE, have been devised with time and are applied to the resolution of proteins on which the performance of IPG-DALT is poor. The difficult-to-handle samples include high pI, high molecular size and high hydrophobicity proteins. The protocol variants entail changes in the gel media and/or the use of unusual additives (often surfactants or solvents). But also: Specific questions require specific answers. When the aim is not the resolution of individual polypeptide chains in a fully unfolded state but the analysis of higher-order structures (proteins assembled from subunits, complexes assembled from interacting proteins) the standard conditions under which IPG-DALT is performed turn inadequate. Electrophoresis is then performed either in first dimension or in both first dimension and second dimension under non-denaturing (native) and/or non-reducing conditions.

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Characterization of allergens from Trisetum paniceum pollen: an important aeroallergen in Mediterranean continental climatic areas

Abstract: Trisetum paniceum pollen contains a high proportion of allergens and these include a group of basic proteins which are not detected in other phylogenetically related pollens and could be of allergological interest.

Cited by 4 publications

References 30 publications

<i>Trisetum paniceum</i> (Wild Oats) Pollen Counts and Aeroallergens in the Ambient Air of Madrid, Spain

<i>Trisetum paniceum</i> (Wild Oats) Pollen Counts and Aeroallergens in the Ambient Air of Madrid, Spain

Cluster immunotherapy with a glutaraldehyde‐modified mixture of grasses results in an improvement in specific nasal provocation tests in less than 2.5 months of treatment

Other than IPG‐DALT: 2‐DE variants

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