Characterization of Allergen (Bet v 1)-Specific T Cell Lines and Clones from Non-Allergic Individuals

Ebner, Christof; Siemann, Ute; Najafian, Nader; Scheiner, Otto

doi:10.1159/000236971

Cited by 17 publications

(13 citation statements)

References 5 publications

(11 reference statements)

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“…During the last decade, clones specific for a variety of allergens have been described, often with heterogeneous cytokine profiles (ie. Th1, Th2, or Th0) [45][46][47][48][49][50][51][52][53]. Indeed, induction of the Th1 cytokine, IFN-γ, is common for a variety of allergens or allergen-derived peptides, irrespective of allergic status [38,44,45,47,[52][53][54][55].…”

Section: Relevance Of the Th1/th2 Paradigm To Allergic Disease In Manmentioning

confidence: 99%

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Woodfolk

2006

CPD

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Production of IgE antibodies promotes the development of allergic disorders such as asthma, rhinitis and atopic eczema. Though Th2 cytokines play a pivotal role in the allergic inflammatory cascade, therapeutic strategies which target these factors have not been curative in clinical trials. In humans, the allergic phenotype encompasses a broad spectrum of diverse clinical entities, which are dictated by genetics as well as the nature of the allergen and the time in life at which allergen is encountered. The disparate findings in animal and human systems highlight the complexity of cytokine-mediated events in allergic responses in man. Different allergic phenotypes cannot be distinguished strictly on the basis of Th1 and Th2 cytokines. These observations coupled with an emerging role for regulatory T cells in modulation of the allergic response warrant re-examination of the cytokine network in allergic disease. This article highlights the challenges of dissecting the role of individual cytokines in the development of allergic responses and manifestation of allergic symptoms. Regulatory T cells have been implicated in modulation of the activity of allergen-specific Th1 and Th2 effector cells and immune outcome; however, the characteristics of these regulatory T cells remain largely undefined. The effects of existing and emerging therapies which target Th1, Th2 and regulatory cytokines on established allergic responses are examined in the context of this new paradigm. Taken together, existing data suggest that a multi-faceted approach, which is tailored to each patient, will be required in order to attain clinical benefit.

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Section: Relevance Of the Th1/th2 Paradigm To Allergic Disease In Manmentioning

confidence: 99%

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Woodfolk

2006

CPD

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“…Nonallergic individuals, however, produce low levels of specific IgG to allergens, a response mediated by Th0/Th1 cells. [189] The modulation of regulatory T cell subsets by adjuvant effects of S layers is a promising concept for immunotherapy and perhaps also for prophylaxis of type I allergies. In the experimental S layer vaccine recombinant birch pollen allergen Bet v 1 (rBet v 1) was conjugated to cross-linked S layers.…”

Section: Surface Layers For Vaccine Developmentmentioning

confidence: 99%

Crystalline Bacterial Cell Surface Layers (S Layers): From Supramolecular Cell Structure to Biomimetics and Nanotechnology

Sleytr

Messner

Pum

et al. 1999

Angewandte Chemie Intl Edit

412

257

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An astonishingly broad application potential in biotechnology, biomimetics, and nanotechnology is revealed by studies on the structure, chemistry, biosynthesis, genetics, self-assembly, and function of supramolecular surface layers (S layers). These are monomolecular, crystalline assemblies of protein or glycoprotein subunits and represent one of the most commonly observed surface structures of prokaryotic cell envelopes (see schematic representation of an archaebacterial cell envelope).

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“…The sensitivity of the assays was 10 pg/ml for both IL-4 and IFN-γ. Th1/2 balance was compared by IL-4/IFN-γ as reported by Ebner et al [16]. …”

Section: Methodsmentioning

confidence: 99%

T Helper Lymphocyte Response to Respiratory Syncytial Virus and Its Components in Patients with Respiratory Allergy and Nonatopic Controls

Shimojo

Katsuki²,

Tateno³

et al. 2008

Int Arch Allergy Immunol

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Background: Respiratory syncytial virus (RSV) G protein is involved in Th2-shifted immune response, while F protein has a reverse effect on RSV infection in Th2-prone BALB/c mice. Studies on the human T cell response to F or G protein are few, and the relationship between the immune response to G protein and atopy is not known. Methods: We established CD4+ RSV-specific T cell lines (TCLs) from adult patients with respiratory allergic diseases (allergics) or nonatopic controls (controls), and examined proliferative responses and γ-interferon (IFN-γ) and interleukin 4 (IL-4) production in these TCLs upon stimulation with RSV, F or G proteins. Results: 32 and 29 RSV-specific oligoclonal TCLs were established from allergics and controls, respectively. IL-4/IFN-γ in the culture supernatant of antigen-stimulated TCLs was significantly higher in allergics than in controls (p = 0.042). IL-4/IFN-γ ratios in the culture supernatants of G-protein-reactive TCLs were significantly higher in allergics than in controls (p = 0.016), while no differences in IL-4/IFN-γ in culture supernatants of F-protein-reactive TCLs were found between allergics and controls (p = 0.787). IL-4/IFN-γ in the culture supernatants of G-protein-reactive TCLs was significantly higher than those of F-protein-reactive TCLs in allergics (p = 0.023) but not in controls (p = 0.768). Conclusion: The results suggest that the T cell response to RSV is influenced by the atopic diathesis as well as by individual RSV antigens, and that G protein may be an important antigen involved in allergy in humans.

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Characterization of Allergen (Bet v 1)-Specific T Cell Lines and Clones from Non-Allergic Individuals

Cited by 17 publications

References 5 publications

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Crystalline Bacterial Cell Surface Layers (S Layers): From Supramolecular Cell Structure to Biomimetics and Nanotechnology

T Helper Lymphocyte Response to Respiratory Syncytial Virus and Its Components in Patients with Respiratory Allergy and Nonatopic Controls

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