Characterization of alginate/poly-l-lysine particles as antisense oligonucleotide carriers

Ferreiro, Marı́a González; Tillman, Lloyd; Hardee, Gregory E.; Bodmeier, Roland

doi:10.1016/s0378-5173(02)00030-3

Cited by 58 publications

(14 citation statements)

References 26 publications

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“…The oil layer was discarded and microcapsules were harvested by centrifugation (350 g , 10 min; SIGMA 3‐30K; Sigma Laborzentrifugen GmbH, Osterode am Harz, Germany). The alginate microcapsules were frozen overnight for 15 h at −20 °C (Fiocchetti, Luzzara, Italy) and lyophilized in a freeze dryer (Alpha 1‐4‐LD Plus; Martin Christ Gefriertrocknungsanlagen GmbH, Osterode am Harz, Germany) at 0.0010 mbar of pressure at −57 °C for 24 h …”

Section: Methodsmentioning

confidence: 99%

Development of caffeine‐encapsulated alginate‐based matrix combined with different natural biopolymers, and evaluation of release in simulated mouth conditions

Mohammadi

Ehsani

Bakhoda

2018

Flavour & Fragrance J

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Caffeine was encapsulated into calcium alginate microcapsules, and these microcapsules were produced with four types of material – starch, xanthan, chitosan, and whey protein isolate – in combination with alginate. Formulation characteristics such as the efficiency of microencapsulation, microcapsule size, viscosity, and texture properties of the microcapsules have been evaluated. The release of caffeine from different microcapsules was conducted in simulated saliva fluid. Alginate microcapsules with chitosan coating provided the slowest release of caffeine, compared with the other microcapsules. Significant differences in the appearance of the microcapsules were detectable by scanning electron microscopy (SEM) and optical microscopy. Fourier‐transform infrared spectrometry (FTIR) analysis confirmed the existence of carbonyl groups in caffeine‐loaded microcapsules, and differential scanning calorimetry (DSC) thermograms indicated the glass transition temperature of the microcapsules. This study showed that the microencapsulation of caffeine with alginate may be helpful for designing fast (alginate) as well as prolonged caffeine release (alginate–starch–xanthan with chitosan).

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Section: Methodsmentioning

confidence: 99%

Development of caffeine‐encapsulated alginate‐based matrix combined with different natural biopolymers, and evaluation of release in simulated mouth conditions

Mohammadi

Ehsani

Bakhoda

2018

Flavour & Fragrance J

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“…At low cationic polymer concentrations, nanospheres are not formed, whereas microspheres are formed at higher concentrations. Alginate nanoparticles can be loaded with oligonucleotides (169,170) and with peptides (167) by means of ionic interactions with the nanoparticle components. In contrast to what was expected, the loading of the alginate nanoparticles with oligonucleotides was mediated by interactions with calcium ions while interactions with polylysine were only marginal (169).…”

Section: One Step Procedures: Methods Based On Ionic Gelationmentioning

confidence: 99%

Methods for the Preparation and Manufacture of Polymeric Nanoparticles

2008

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This review summarizes the different methods of preparation of polymer nanoparticles including nanospheres and nanocapsules. The first part summarizes the basic principle of each method of nanoparticle preparation. It presents the most recent innovations and progresses obtained over the last decade and which were not included in previous reviews on the subject. Strategies for the obtaining of nanoparticles with controlled in vivo fate are described in the second part of the review. A paragraph summarizing scaling up of nanoparticle production and presenting corresponding pilot set-up is considered in the third part of the review. Treatments of nanoparticles, applied after the synthesis, are described in the next part including purification, sterilization, lyophilization and concentration. Finally, methods to obtain labelled nanoparticles for in vitro and in vivo investigations are described in the last part of this review.

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“…This method is advantageous by virtue of being a simple method that does not involve expensive equipment, harsh processing conditions, or organic solvent systems. Recently, alginate-based nanoparticles were reported to protect an associated oligonucleotide and, as a potential carriers, to enhance the oral bioavailability of antisense therapeutics (21). No data on use of pectinate micro/nanoparticles as a non-viral vector is available.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

et al. 2008

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Abstract. The aim of this study was to investigate the possibility of using pectinate micro/nanoparticles as gene delivery systems. Pectinate micro/nanoparticles were produced by ionotropic gelation. Various factors were studied for their effects on the preparation of pectinate micro/nanoparticles: the pH of the pectin solution, the ratio of pectin to the cation, the concentration of pectin and the cation, and the type of cation (calcium ions, magnesium ions and manganese ions). After the preparation, the size and charge of the pectin micro/nanoparticles and their DNA incorporation efficiency were evaluated. The results showed that the particle sizes decreased with the decreased concentrations of pectin and cation. The type of cations affected the particle size. Sizes of calcium pectinate particles were larger than those of magnesium pectinate and manganese pectinate particles. The DNA loading efficiency showed that Capectinate nanoparticles could entrap DNA up to 0.05 mg when the weight ratio of pectin:CaCl 2 :DNA was 0.2:1:0.05. However, Mg-pectinate could entrap only 0.01 mg DNA when the weight ratio of pectin: MgCl 2 :DNA was 1:100:0.01 The transfection efficiency of both Ca-pectinate and Mg-pectinate nanoparticles yielded relatively low levels of green fluorescent protein expression and low cytotoxicity in Huh7 cells. Given the negligible cytotoxic effects, these pectinate micro/nanoparticles can be considered as potential candidates for use as safe gene delivery carriers.

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Characterization of alginate/poly-l-lysine particles as antisense oligonucleotide carriers

Cited by 58 publications

References 26 publications

Development of caffeine‐encapsulated alginate‐based matrix combined with different natural biopolymers, and evaluation of release in simulated mouth conditions

Development of caffeine‐encapsulated alginate‐based matrix combined with different natural biopolymers, and evaluation of release in simulated mouth conditions

Methods for the Preparation and Manufacture of Polymeric Nanoparticles

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

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