Characterization of adenosine receptors in bovine chondrocytes and fibroblast-like synoviocytes exposed to low frequency low energy pulsed electromagnetic fields

Varani, Katia; Mattei, Monica De; Vincenzi, Fabrizio; Gessi, Stefania; Merighi, Stefania; Pellati, Agnese; Ongaro, Alessia; Caruso, Angelo; Cadossi, Ruggero; Borea, Pier Andrea

doi:10.1016/j.joca.2007.07.004

Cited by 113 publications

(129 citation statements)

References 45 publications

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“…Chondrocytes are known to express all the P1 receptors (Tesch et al, 2002;Varani et al, 2008a); however, information regarding the functional effects of receptor activation remains limited. Pulsed electro-magnetic fields have been shown to promote chondrocyte proliferation via the up-regulation of A2A and A3 receptor expression (Varani et al, 2008a).…”

Section: P1 Receptors and Chondrocytesmentioning

confidence: 99%

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The role of purinergic signalling in the musculoskeletal system

Orriss

2015

Autonomic Neuroscience

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Section: P1 Receptors and Chondrocytesmentioning

confidence: 99%

“…Pulsed electro-magnetic fields have been shown to promote chondrocyte proliferation via the up-regulation of A2A and A3 receptor expression (Varani et al, 2008a). Adenosine, acting via A2A receptors, can also reduce IL-1β-induced inflammation (Campo et al, 2012) and VEGF secretion (Vincenzi et al, 2013).…”

Section: P1 Receptors and Chondrocytesmentioning

confidence: 99%

The role of purinergic signalling in the musculoskeletal system

Orriss

2015

Autonomic Neuroscience

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“…Several investigations have been carried out to assess the efficacy of PEMFs in patients with OA but the results have been contradictory [20,22]. Thamsborg et al 18 conducted a Randomized Clinical Trial (RCT) including 83 patients and found no significant differences in the outcome scores in the group treated with PEMFs compared to a placebo group [18].…”

Section: Discussionmentioning

confidence: 99%

“…In a RCT evaluating the outcomes of arthroscopic chondro-abrasion or perforation followed by treatment with PEMF, Zorzi et al showed that PEMFs aided patient recovery by reducing the requirement of analgesics. The use of PEMFs was associated with improved functional outcomes associated with a long-term effects [22]. However, the cartilage procedure creates a confounding factor and questions the actual benefit of PEMF in the long run.…”

Section: Discussionmentioning

confidence: 99%

Is There any Role for Pulsed Electromagnetic Fields in the Treatment of Early Osteoarthritis of the Knee?

Petrera¹

2013

J Osteopor Phys Act

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Volume 1 • Issue 2 • 1000106institutional review board approval. A written, informed consent was obtained from all the patients. 53 patients with symptomatic cartilage lesions of the knee were treated with PEMFs at our institute. 28 of them (13 male and 15 female) met our study inclusion criteria and were prospectively followed up for a minimum of 2-years post treatment. Inclusion criteria-age between 30 and 60 years; -grade 1-2 cartilage lesion according to the ICRS classification, evaluated by MRI and previous diagnostic arthroscopy ± lavage within 6 months prior to start of treatment ( AbstractObjective: Pulsed Electromagnetic Fields (PEMFs) were introduced in the clinical setting in the 1970s. Proven to be a successful method of treating non-union and delayed union of fractures, its effects on cartilage has remained ambiguous. PEMFs have demonstrated a pro-anabolic and anti-catabolic activity on cartilage metabolism. We hypothesized that the use of PEMFs in patients with symptomatic cartilage lesions of the knee would lead to improved clinical outcomes in an observational study to evaluate the results after 2-years.Methods: 25 patients between the age of 30 and 60 years who underwent treatment with PEMFs for symptomatic cartilage lesions of the knee (grade 1-2 as per ICRS classification) were included in this prospective case series. Patients were evaluated pre-treatment, at 12 months and 24 months using Visual Analogue Scale (VAS) scale for pain, Tegner and KOOS scores.. Results:A significant improvement in all scores was observed at 1-year follow-up (p=0.003). At 2-year follow-up, results deteriorated but were still superior to pre-treatment levels (p=0.04). No adverse reactions were seen. Conclusions:PEMFs in patients with symptomatic isolated cartilage lesions of the knee can cause improvement in symptoms, knee function and activity in the short term. Repetition of treatment annually may improve the long term results.

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“…Later, it was demonstrated that chondrocytes respond to adenosine via A 2 receptors, activity which was potentiated by adenosine deaminase (ADA) and phosphodiesterase inhibitors [337]. Furthermore, pulsed electromagnetic fields produce an up-regulation of the A 2A and A 3 receptors that mediate proliferation of bovine chondrocytes and fibroblast-like synoviocytes [338], and A 2A receptor activation reduces inflammation in mouse articular chondrocytes stimulated with IL-1β [339].…”

Section: P1 Receptors Adenosine and Chondrocytesmentioning

confidence: 99%

Purinergic signalling in the musculoskeletal system

Burnstock

Arnett

Orriss

2013

Purinergic Signalling

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It is now widely recognised that extracellular nucleotides, signalling via purinergic receptors, participate in numerous biological processes in most tissues. It has become evident that extracellular nucleotides have significant regulatory effects in the musculoskeletal system. In early development, ATP released from motor nerves along with acetylcholine acts as a cotransmitter in neuromuscular transmission; in mature animals, ATP functions as a neuromodulator. Purinergic receptors expressed by skeletal muscle and satellite cells play important pathophysiological roles in their development or repair. In many cell types, expression of purinergic receptors is often dependent on differentiation. For example, sequential expression of P2X5, P2Y 1 and P2X2 receptors occurs during muscle regeneration in the mdx model of muscular dystrophy. In bone and cartilage cells, the functional effects of purinergic signalling appear to be largely negative. ATP stimulates the formation and activation of osteoclasts, the bone-destroying cells. Another role appears to be as a potent local inhibitor of mineralisation. In osteoblasts, the bone-forming cells, ATP acts via P2 receptors to limit bone mineralisation by inhibiting alkaline phosphatase expression and activity. Extracellular ATP additionally exerts significant effects on mineralisation via its hydrolysis product, pyrophosphate. Evidence now suggests that purinergic signalling is potentially important in several bone and joint disorders including osteoporosis, rheumatoid arthritis and cancers. Strategies for future musculoskeletal therapies might involve modulation of purinergic receptor function or of the ecto-nucleotidases responsible for ATP breakdown or ATP transport inhibitors.

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Characterization of adenosine receptors in bovine chondrocytes and fibroblast-like synoviocytes exposed to low frequency low energy pulsed electromagnetic fields

Cited by 113 publications

References 45 publications

The role of purinergic signalling in the musculoskeletal system

The role of purinergic signalling in the musculoskeletal system

Is There any Role for Pulsed Electromagnetic Fields in the Treatment of Early Osteoarthritis of the Knee?

Purinergic signalling in the musculoskeletal system

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