Characterization of Acinetobacter baumannii from water and sludge line of secondary wastewater treatment plant

Higgins, Paul G.; Hrenović, Jasna; Seifert, Harald; Dekić, Svjetlana

doi:10.1016/j.watres.2018.04.057

Cited by 85 publications

(44 citation statements)

References 20 publications

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“…2). IC5 has been found in North, Central, and South America and has been considered a Pan-American clone; notably, it very recently has been described in southeastern Europe as well (27). However, there are 11 Honduran isolates and two Mexican isolates that are assigned to other distantly related clades within the tree, demonstrating that several clones coexist in these countries.…”

Section: Resultsmentioning

confidence: 99%

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Graña-Miraglia

Evans

López‐Jácome

et al. 2020

mSphere

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Over the last few decades, carbapenemase-producing Acinetobacter baumannii has become a major cause of nosocomial infections all over the world. However, the genome identity of lineages of this species in Latin America has not been studied as much as in developed countries. Here, through a population genomics approach considering the whole genomes of 148 isolates (almost 40 from Mexico and Honduras), we describe the recent emergence of the lineage sequence type 758 (ST758), which belongs to the international clone V and has spread out to Canada, Mexico, Honduras, and Colombia. Notably, this lineage was found to coexist with other A. baumannii lineages in hospitals in Mexico and Honduras. Isolates from this lineage show considerable variation in antibiotic resistance profiles, but most of them are resistant to carbapenems. Moreover, we found a variety of acquired oxacillinase (OXA) families within this lineage and tracked the very recent inception, and subsequent horizontal transmission, of the OXA-239 carbapenemase. This work highlights the urgent need to investigate recently emerged lineages of this species in Latin America and elsewhere, as these might harbor novel antibiotic resistance genes. IMPORTANCE A. baumannii is a major cause of nosocomial infections all over the world. Although many isolates from developed countries have been studied in terms of their genome sequence, isolates from Latin America have been much less studied. In this study, using a population genomics approach considering the whole genomes of 148 isolates, we describe the recent emergence of the lineage ST758 endemic to Latin America and the inception of the OXA-239 carbapenemase. Our study highlights the urgent need to investigate recently emerged lineages of this species in Latin America and elsewhere, as these might harbor novel antibiotic resistance genes.

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Section: Resultsmentioning

confidence: 99%

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Graña-Miraglia

Evans

López‐Jácome

et al. 2020

mSphere

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“…In fact, the PM > 1 concentration of bacterial cell in WWTP_RE2 was six times higher than WWTP_RE1. The existing differences were due to an advection episode of air masses coming from the desert areas of North Africa-these masses hit Central Italy during those monitoring days (11)(12)(13)(14)(15)(16)(17). According to the literature, this type of event has a positive effect on bacterial growth and abundance.…”

Section: Bacterial Cells and Fungal And Bacterial Spores By Chemical mentioning

confidence: 99%

“…Assessments of the hazard of bioaerosol exposure have usually focused on identifying the aerosolized pathogens that are produced in the aeration basins from WWT, such as Klebsiella pneumoniae, Legionella sp., Acinetobacter sp. and Mycobacterium tuberculosis, producing a serious hazard to on-site operators and adjacent communities [8][9][10][11][12][13]. Furthermore, WWTPs are the main source of antibiotic releases in the environment, and they contribute to the dissemination of antibiotic resistance genes (ARGs) among bacteria, many of which are pathogenic to humans [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

Bruni

Simonetti

Bovone

et al. 2019

IJERPH

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Wastewater carries different pathogenic and non-pathogenic microorganisms that can be dispersed in the surrounding environment. Workers who frequent sewage treatment plants can therefore be exposed to aerosols that contain a high concentration of potentially dangerous biological agents, or they can come into direct contact with contaminated material. This can lead to allergies, infections and occupational health-associated diseases. A characterization of biological risk assessment of bioaerosol exposure is necessary. The aim of this study was to evaluate the application of an interdisciplinary method that combines chemical and biological approaches for the analysis of a bioaerosol derived from a wastewater treatment plant (WWTP) situated in Italy. Sampled filters were analyzed by HPLC-MS/MS spectroscopy that searched for different chemical biomarkers of airborne microorganisms. The analytical quantification was compared to the biological cultural method that revealed an underrated microbial concentration. Furthermore, next generation sequencing analysis was used also to identify the uncultivable species that were not detected by the culture dependent-method. Moreover, the simple animal model Caenorhabditis elegans was used to evaluate the pathogenicity of two isolates—Acinetobacter iwoffii and Micrococcus luteus—that showed multidrug-resistance. This work represents a starting point for the development of a multidisciplinary approach for the validation of bioaerosol exposure on WWTP workplaces.

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“…Therefore, the ability to remove antibiotics is limited. The traditional sewage treatment plant includes pre-treatment, primary stage treatment, two stages treatment system [8][9] and a conditional sewage treatment plant will also start a three stages treatment program, such as chemical oxidation, activated carbon adsorption or ultraviolet oxidation [10][11][12]. The pre-treatment and primary stage treatment processes are aimed at removing organic suspension which is large and easy to settle.…”

Section: Introductionmentioning

confidence: 99%

Enhanced adsorption of sulfonamide antibiotics in water by modified biochar derived from bagasse

Qin¹,

Huang

Tang³

et al. 2019

Open Chemistry

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In this study, biochars derived from bagasse were prepared and their ability for the adsorption of four kinds of sulfonamide antibiotics (sulfamethoxazol, thiazole, methylpyrimidine, dimethylpyrimidine) was investigated. Results showed that the modified biochar can efficiently adsorb sulfonamides in water. The biochar obtained at 500oC and modified with 30% hydrogen peroxide was chosen as the adsorbent. Under optimum conditions, pH 4 and 35°C, great adsorption performance was exhibited in the adsorption process of the four sulfonamide antibiotics. The productivity of the modified biochar was ~ 89% compared to un-modified biochar which is ~31%. The successful preparation of biochar from bagasse indicates that it is a good way to reuse the resources. Besides the adsorption of antibiotics, the obtained material also has a great prospect in the removal of other pollutants.

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Characterization of Acinetobacter baumannii from water and sludge line of secondary wastewater treatment plant

Cited by 85 publications

References 20 publications

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

Enhanced adsorption of sulfonamide antibiotics in water by modified biochar derived from bagasse

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