Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos of Solanum chacoense

Lagacé, Marie Claude; Matton, Daniel P.

doi:10.1007/s00425-004-1253-2

Cited by 147 publications

(86 citation statements)

References 21 publications

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“…The WRKY superfamily of plant transcriptional factors has been implicated in a variety of physiological and developmental processes unique to plants, including leaf senescence (28,29), trichome initiation (30), and embryo morphogenesis (31). The WRKY domain functions through direct interactions with the W-box domain in the promoter region of downstream gene targets (23).…”

Section: Discussion Evolution Of Sbwrky Orthologs In Two Sorghum Specmentioning

confidence: 99%

Seed shattering in a wild sorghum is conferred by a locus unrelated to domestication

Tang

Cuevas

Das

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Suppression of seed shattering was a key step during crop domestication that we have previously suggested to be convergent among independent cereal lineages. Positional, association, expression, and mutant complementation data all implicate a WRKY transcription factor, SpWRKY, in conferring shattering to a wild sorghum relative, Sorghum propinquum. We hypothesize that SpWRKY functions in a manner analogous to Medicago and Arabidopsis homologs that regulate cell wall biosynthesis genes, with low expression toward the end of floral development derepressing downstream cell wall biosynthesis genes to allow deposition of lignin that initiates the abscission zone in the seed-pedicel junction. The recent discovery of a YABBY locus that confers shattering within Sorghum bicolor and other cereals validated our prior hypothesis that some parallel domestication may have been convergent. Ironically, however, the shattering allele of SpWRKY appears to be recently evolved in S. propinquum and illustrates a case in which the genetic control of a trait in a wild relative fails to extrapolate even to closely related crops. Remarkably, the SpWRKY and YABBY loci lie only 300 kb apart and may have appeared to be a single genetic locus in some sorghum populations.cereal crop | seed dispersal | association mapping

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Section: Discussion Evolution Of Sbwrky Orthologs In Two Sorghum Specmentioning

confidence: 99%

Seed shattering in a wild sorghum is conferred by a locus unrelated to domestication

Tang

Cuevas

Das

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…Analyses of plant databases have revealed the existence of more than 500 WRKY ESTs identified from various tissue sources, including roots, leaves, inflorescences, seeds and vascular tissue (Eulgem et al 2000). There are also reports in various plant species that some members of the WRKY family play key roles in plant development, such as during morphogenesis (Johnson et al 2002), embryo development (Lagace and Matton 2004) and senescence (Gregersen and Holm 2007). The constitutive expression of WRKY45 genes in wheat and rice suggests that these genes might also play an important role, not only under conditions of stress but also during normal plant development.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a wheat transcription factor, TaWRKY45, and its effect on Fusarium head blight resistance in transgenic wheat plants

et al. 2011

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The WRKY transcription factors belong to a large protein family characterized by the conserved WRKY domain. These factors have been identified to play biological functions in various plant developmental processes. WRKY proteins are also known to be involved in regulating plant responses to pathogen attack and stressrelated hormones. In this study, we report the isolation and characterization of the gene (TaWRKY45) for the wheat WRKY45 transcription factor. Amino acid sequence alignment and phylogenetic analyses demonstrated that the TaWRKY45 protein is orthologous to rice OsWRKY45. Our analysis of its expression in wheat indicated that TaWRKY45 was constitutively expressed in various organs and throughout the lifetime of the plant. We observed that TaWRKY45 was upregulated in response to benzothiadiazole (BTH), a plant immune system strengthner, and Fusarium graminearum, which is a causal fungus for Fusarium head blight (FHB). The constitutive overexpression of the TaWRKY45 transgene conferred an enhanced resistance against F. graminearum to transgenic wheat plants grown under greenhouse conditions. These results indicate that TaWRKY45 is involved in the defense systems for the biotic stressors in wheat and that it may be potentially utilized to improve the disease resistance of wheat.

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“…There are 74 members of this gene family in Arabidopsis and 109 in rice (Eulgem and Somssich 2007;Ross et al 2007). They are believed to be involved in regulation of several physiological processes in plants, such as immunity (Pandey and Somssich 2009), embryogenesis (Lagacé and Matton 2004), hormonal signaling (Zhang et al 2004;Xie et al 2005), trichome and seed coat development (Johnson et al 2002), regulation of biosynthesis pathways (Xu et al 2004), and senescence (Lin and Wu 2004;Buchanan-Wollaston et al 2005). WRKY transcription factors play a role in plant immunity by regulating the expression of defense response genes (Eulgem et al 2000;Heise et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Identification of Differentially Expressed Genes during Lace Plant Leaf Development

Rantong

Kelen

Breusegem

et al. 2016

International Journal of Plant Sciences

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Editor: John L. Bowman Premise of research. The lace plant is an excellent and unique model for studying developmentally regulated programmed cell death (PCD) in plants. Perforations form in highly predictable and easily accessible and distinguishable areas in lace plant leaves. However, little is known about the genes involved in regulation of this PCD or leaf development. In this study, for the first time, a general gene expression profile for lace plant leaf development was investigated.Methodology. A cDNA-amplified fragment length polymorphism involving 64 primer combinations was used for a half-genome analysis of 4666 transcripts. Two hundred and thirty differentially expressed transcriptderived fragments (TDFs) were sequenced. A partial expressed sequence tag (EST) database for window-stage (in which PCD is occurring) leaves was also established. Through a reverse transcription polymerase chain reaction, the possible role of ubiquitin in lace plant PCD was investigated.Pivotal results. Seventy-nine TDFs were successfully annotated. The isolated TDFs and ESTs encoded genes involved in processes such as photosynthesis, biosynthesis pathways, gene regulation, stress responses, defense against pathogens, and PCD, among others. Indirect evidence through ubiquitin transcript levels suggests involvement of proteasome machinery in lace plant development and PCD. This study provides a foundation for selective studies on regulation of lace plant leaf development and PCD.

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Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos of Solanum chacoense

Cited by 147 publications

References 21 publications

Seed shattering in a wild sorghum is conferred by a locus unrelated to domestication

Seed shattering in a wild sorghum is conferred by a locus unrelated to domestication

Characterization of a wheat transcription factor, TaWRKY45, and its effect on Fusarium head blight resistance in transgenic wheat plants

Identification of Differentially Expressed Genes during Lace Plant Leaf Development

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