Characterization of a wheat–Thinopyrum bessarabicum (T2JS-2BS·2BL) translocation line

Qi, Zengjun; Du, Peijun; Baoli, Qian; Zhuang, Lifang; Huafeng, Chen; Chen, Tingting; Shen, Jian; Guo, Jie; Feng, Yue; Ziyou, Pei

doi:10.1007/s00122-010-1332-7

Cited by 47 publications

(24 citation statements)

References 25 publications

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“…The transfer of desirable genes from the tertiary gene pool could be an efficient way to increase genetic diversity and improve cultivated wheat [28,48]. Translocation lines, especially small alien segment translocations, would be genetically more stable and desirable [49].…”

Section: Discussionmentioning

confidence: 99%

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Luan

Wang

Liu

et al. 2010

Planta

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Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable traits that can be transferred to common wheat through breeding programs. The wheat-A. cristatum disomic addition and translocation lines can be used as bridge materials to introduce alien chromosomal segments to wheat. Wheat-A. cristatum 2P disomic addition line II-9-3 was highly resistant to powdery mildew and leaf rust, which was reported in our previous study. However, some translocation lines induced from II-9-3 have not been reported. In this study, some translocation lines were induced from II-9-3 by 60 Co-γ irradiation and gametocidal chromosome 2C and then identified by cytological methods. Forty-nine wheat-A. cristatum translocation lines were obtained and various translcoation types were identified by GISH (genomic in situ hybridization), such as whole-arm, segmental and intercalary translocations. Dual-color FISH (fluorescent in situ hybridization) was applied to identify the wheat chromosomes involved in the translocations, and the results showed that A. cristatum 2P chromosome segments were translocated to the different wheat chromosomes, including 1A, 2A, 3A, 4A, 5A, 6A, 7A, 3B, 5B, 7B, 1D, 4D and 6D. Many different types of wheat-A. cristatum alien translocation lines would be valuable for not only identifying and cloning A. cristatum 2P-related genes and understanding the genetics and breeding effects of the translocation between A. cristatum chromosome 2P and wheat chromosomes, but also providing new germplasm resources for the wheat genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Luan

Wang

Liu

et al. 2010

Planta

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“…Of the eight CS-Th. bessarabicum alien chromosome lines, DS1J(1B) (Tan 2011), DS5J(5A) (Dawadondup 2009), and DS6J(6A) (Shen 2011), were disomic substitutions (DS); DA4J, DA3J, DA6JSÁ2JL, and DA7J (Zhuang et al 2003(Zhuang et al , 2004Chen et al 2007;Shen 2011) were disomic additions (DA); and T2JS-2BSÁ2BL was a translocation line where a segment of chromosome arm 2JS was translocated to chromosome arm 2BS (Qi et al 2010).…”

Section: Plant Materialsmentioning

confidence: 98%

Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum

Shen

Dawadondup

et al. 2012

Mol Breeding

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Blue wheat grain contains different groups of pigments that can be used for making specialty foods or as food colorants. Thinopyrum bessarabicum, a wild relative of wheat, carries a blue-grained gene on chromosome 4J. In this study, we analyzed the mitotic chromosomes of 159 F7 lines derived from the cross between Triticum aestivum cv. Chinese Spring (CS) and a CS-Th. bessarabicum amphiploid by using multi-color fluorescence in situ hybridization, genomic in situ hybridization, and newly developed chromosome 4J-specific DNA markers. Intact chromosome 4J and various 4J chromosomal segments were identified in the 159 lines. The blue-grained gene of Th. bessarabicum was physically localized to the region between the centromere and FL0.52 on chromosome arm 4JL. The chromosomal location of this gene differed from the location of previously reported blue-grained genes. In addition, a strong dosage effect was observed with this gene. These results suggest that the blue-grained gene in Th. bessarabicum represents a novel gene locus for blue aleurone, designated BaThb. The wheat lines and 4J chromosome-specific molecular markers developed in this study will facilitate the introgression and utilization of BaThb for wheat nutritional quality improvement.

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“…A translocation line T2JS-2BS.2BL involving chromosome 2J of Th. bessarabicum was developed and characterized by fluorescent in situ hybridization (FISH) (Qi et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations

et al. 2015

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Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is a valuable source of genes for bread wheat (2n = 6x = 42) improvement because of its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines by backcrossing the amphiploid in the absence of the Ph1 gene (allowing intergenomic recombination) can assist its utilization in wheat improvement. In this study, six novel wheat-Th. bessarabicum translocation lines involving different chromosome segments (T4BS.4BL-4JL, T6BS.6BL-6JL, T5AS.5AL-5JL, T5DL.5DS-5JS, T2BS.2BL-2JL, and the whole arm translocation T1JS.1AL) were identified and characterized using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). No background translocations between wheat genomes were observed. The involvement of five of the seven chromosomes and small terminal segments of Th. bessarabicum chromosome arm were important, contributing to both reduced linkage drag of the derived lines by minimizing agronomically deleterious genes from the alien species and high stability including transmission of the alien segment. All three wheat genomes were involved in the translocations with the alien chromosome, and GISH showed the Th. bessarabicum genome was more closely related to the D genome in wheat. All the introgression lines were disomic, stable, and with good morphological characters.

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Characterization of a wheat–Thinopyrum bessarabicum (T2JS-2BS·2BL) translocation line

Cited by 47 publications

References 25 publications

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum

Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations

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