Characterization of a wheat-Psathyrostachys huashanica Keng 4Ns disomic addition line for enhanced tiller numbers and stripe rust resistance

Abstract

Exploiting and utilizing excellent gene(s) from wild species has become an essential strategy for wheat improvement. In the disomic addition line 24-6-3, the 4Ns chromosomes from Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) carried valuable tiller and strip rust resistance gene(s), which was selected from the progeny of common wheat cv. 7182 and P. huashanica via embryo culture. Cytology, genomic in situ hybridization (GISH), and EST-STS analyses were used to detect the 4Ns chromosome in wheat background and its homoeologous relationship. Cytological studies demonstrated that 24-6-3 contained 44 chromosomes and formed 22 bivalents during meiotic metaphase I. GISH using P. huashanica genomic DNA as a probe indicated that a pair of Ns-chromosomes with strong hybridization signals had been introduced into 24-6-3. Ten EST-STS markers, i.e., BE404973, BE442811, BE446061, BE446076, BE497324, BE591356, BF473854, BG274986, BQ161513 and CD373484, which were located on the homoeologous group 4 chromosomes of wheat, amplified bands unique to P. huashanica in 24-6-3. This indicated the presence of an introgressed P. huashanica Ns chromosome pair belonging to homoeologous group 4, which we designated the 4Ns disomic addition line. After it was inoculated using mixed races of stripe rust in the adult stages, 24-6-3 expressed high stripe rust resistance, which was possibly derived from its P. huashanica parent. Moreover, its increased number of tillers was probably controlled by gene(s) located in P. huashanica chromosome 4Ns. These high levels of disease resistance and excellent agronomic traits make the 24-6-3 line a promising germplasm for breeding in wheat.

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

“…For example, a wheat-P. huashanica 1Ns disomic addition line exhibits increased storage of microelements in the seeds [10]. In addition, 2Ns, 3Ns, 4Ns, and 5Ns disomic addition lines and a 2Ns(2D) substitution line are resistant to stripe rust [13][14][15][16]18]. A 6Ns disomic addition line and a small segment translocation line possess twin spikelets and more kernels per spike than its wheat parent [13,21], and a 7Ns disomic addition line showed high resistance to leaf rust [12].…”

“…The colchicine treated H811 was crossed and backcrossed to 7182 to get heptaploid hybrid H8911 (2n = 49, AABBDDNs). Subsequently, H8911 was then backcrossed to 7182 or crossed to several other wheat cultivars, and generated a series of wheat-P. huashanica derived lines including wheat-P. huashanica 1Ns-7Ns disomic addition lines [12][13][14][15][16][17], 2Ns(2D) disomic substitution line [18], 5Ns(5D) disomic substitution line [19], and translocation lines [20,21]. These wheat-P. huashanica derived lines have more desired agronomic traits than their wheat parents, demonstrating that P. huashanica is a useful wild relative for wheat improvement.…”

Development and molecular cytogenetic identification of a new wheat–Leymus mollis Lm#6Ns disomic addition line

“…) and Psathyrostachys huashanica Keng (Du et al. ,c, ). Until now, none of wheat– L. mollis disomic addition lines has been developed and reported in detail.…”

“…A. L€ ove (Jauhar et al 2009), Thinopyrum intermedium (Larkin et al 1995), Secale cereale (Zhuang et al 2011), Leymus racemosus (Qi et al 2008), Agropyron cristatum (L.) Gaertn (Wu et al 2006) and Psathyrostachys huashanica Keng (Du et al 2013a(Du et al ,c, 2014. Until now, none of wheat-L. mollis disomic addition lines has been developed and reported in detail.…”

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and <b><i>Psathyrostachys huashanica</i></b>

“…In order to transfer desirable traits from P. huashanica into wheat, wide crosses between P. huashanica and common wheat began in the 1980s [Chen et al, 1991]. Progeny lines with superior disease resistance and agronomic traits were produced either as amphiploids or chromosome addition, substitution, and translocation lines [Kang et al, 2009[Kang et al, , 2010[Kang et al, , 2011Kishii et al, 2010;Zhao et al, 2010;Du et al, 2013Du et al, , 2014.…”