To analyze the biological effects of radiation, it is important that the
conditions of in vitro experiments match closely with those of in vivo
experiments. In this study, we constructed an irradiation system to
conduct irradiation experiments under conditions similar to those of in vivo
experiments. The Dongnam Institute of Radiological and Medial Sciences has a
gamma irradiator including 60Co radioisotope for research purposes and
accreditation for standard calibration of the ion chamber. The temperature
of the water phantom was maintained the same as that of the normal human
body, and the physical dosimetry was carried out accurately using the ion
chamber with traceability. We report the measurement of lateral profiles,
depth profiles, and absorbed dose rate in water, Dw, at the irradiation
location of the blood samples using a farmer-type ion chamber. We simulated
the source, collimator, irradiator, phantom, and extra structure of the
gamma irradiation system using the Monte Carlo code and compared the
simulated and the experimental results. The experimentally and theoretically
evaluated dose rates were 0.2975 ? 0.0055 Gymin?1 (at coverage factor k =
2) and 0.2978 ? 0.0052 Gymin?1 (at coverage factor k = 2) at
source-to-surface distance of 100 cm and 5 gcm?2 depth in the water phantom,
respectively. Blood irradiation will be conducted in vitro, under conditions similar to in vivo conditions, to provide the dose-response curve
based on dosimetry with traceability.