Characterization of a type‐C virus produced by co‐cultures of human leukemic bone‐marrow and fetal canine thymus cells

Smith, R G; Nooter, Kees; Bentvelzen, P.; Robert-Guroff, Marjorie; Harewood, Ken R.; Reitz, Marvin S.; Lee, Sin Ai; Gallo, Robert C.

doi:10.1002/ijc.2910240213

Cited by 10 publications

(10 citation statements)

References 48 publications

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“…From our results we conclude that the p 30 polypeptides of SKA21-3 and SSV have identical primary structures, confirming the relatedness of these proteins as detected before by immunological methods (2,3).…”

supporting

confidence: 91%

Structural characterization of the major internal protein of the SKA 21-3 virus

Brouwer

Dulk

1981

Archives of Virology

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supporting

confidence: 91%

Structural characterization of the major internal protein of the SKA 21-3 virus

Brouwer

Dulk

1981

Archives of Virology

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“…Retroviruses have been isolated from several leukaemic patients by co-cultivation of tumour tissues with mammalian cell lines (Gallagher & Gallo, 1975;Panem et al, 1975;Smith et al, 1979;Gabelman et al, 1975;Kaplan et al, 1977). The best studied of these human virus isolates is HL23 virus (Gallagher & Gallo, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…Several of the human retrovirus isolates, notably HL23 virus (Gallagher & Gallo, 1975), HELl2 virus (Panem et aL, 1975), A1476 virus (Smith et aL, 1979), DHL-1 virus (Goodenow & Kaplan, 1979) and SKA21-3 virus , are immunologically related to the horizontally transmitted family of non-human primate retroviruses, simian sarcomasimian-associated virus [SiSV(SiAV)]/gibbon ape leukaemia viruses (GaLV). SiSV(SiAV)/ GaLV are the only known retroviruses clearly associated with neoplastic transformation in primates (Wolfe et al, 1971;Sun et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Intracellular Precursor Polyproteins of Simian Sarcoma-associated Virus [SiSV(SiAV)] and Three Human Virus Isolates: HL23V, HEL12V and A1476V

Fuqua

Naso

1982

Journal of General Virology

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SUMMARYThe intracellular precursor polyproteins of simian sarcoma-simian-associated virus [SiSV(SiAV)] were compared to the intracellular proteins of the human retrovirus isolates, HL23V, HEL12V and A1476V, by radioimmunoprecipitation followed by SDS-polyacrylamide gel electrophoresis and tryptic peptide analysis. Cells infected with SiSV(SiAV) were characterized by polyproteins Pr200 gas-p°), gPr80 env, Pr80 gag, Pr60 gag and Pr40 gag. Identical intracellular precursor polyprotein profiles were obtained from cells infected with HL23V, HEL12V and A1476V. Tryptic digest mapping of peptides containing [3H]leucine showed the structural composition of Pr60 gag to be the virus core proteins, p28, p15/p12 and pl0. The SiSV(SiAV) envelope precursor, gPr80 env, contained the structural determinants of mature viral gp70 and a non-glycosylated protein termed p15E. The homology of the human isolate viruses, HL23V, HEL12V and A1476V, to the SiSV(SiAV)/ GaLV (gibbon ape leukaemia virus) family of viruses was confirmed by mapping studies. Both gPr80 env and Pr60 gag of SiAV were identical by tryptic peptide mapping to the respective proteins from the three human retrovirus isolates examined. The potential significance of these results to considerations of the origins of SiAV and the SiAV-like human isolates is discussed.

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“…Cell-cell interactions are also important to the differentiated cellular derivatives of stem cells. Immunological studies commonly utilize co-culture to evaluate the ability of immunocytes to recognize and attack particular target cells (139)(140)(141)(142)(143)(144)(145)(146)(147)(148)(149)(150)(151)(152). Cell-cell interactions in v i m among committed hemopoietic cells involve co-cultured lymphocytes and/or macrophages.…”

Section: Hemopoietic Cellsmentioning

confidence: 99%