Characterization of a trehalose-6-phosphate synthase gene from Spodoptera exigua and its function identification through RNA interference

Tang, Bin; Chen, Jie; Yao, Qiong; Pan, Zhanqing; Xu, Wei‐Hua; Wang, Shigui; Zhang, Wenqing

doi:10.1016/j.jinsphys.2010.02.009

Cited by 76 publications

(100 citation statements)

References 34 publications

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“…It is reported that SexTPS is specifically expressed in the fat body and ovaries in larval stages, which is consistent with the results of studies on H. armigera (Xu et al, 2009, Tang et al, 2010. In Locusta migratoria, TPS is expressed not only in the fat body, but also in gut, hemolymph, and leg muscle (Cui & Xia, 2009).…”

Section: Discussionsupporting

confidence: 88%

“…In D. melanogaster, a mutation in the TPS gene results in young larvae dying, which indicates that the TPS gene plays a critical role in insect development (Chen et al, 2002). SexTPS RNAi results also suggest that interference with TPS expression results in the death of larvae (Tang et al, 2010). Thus, TPS is a potential key gene that might also be used to control insect pests.…”

Section: Discussionmentioning

confidence: 94%

“…In addition, in S. exigua, semiquantitative RT-PCR results reveal that SexTPS mRNA is expressed in fat body at different levels from fifth instar larvae to pupae (Tang et al, 2010). Howerver, different patterns of expression of CpiTPS were found and studied (Fig.…”

Section: Discussionmentioning

confidence: 94%

“…3´-RACE was performed using 2.5 µl of 3´-ready-cDNA with UPM and CpiTPS-3F1, then with NUP and CpiTPS-3F2. The PCR conditions were as follows: 10 min at 94°C, followed by 30 cycles of 30 s at 94°C, 30 s at 60°C, and 80 s at 72°C, then 10 min at 72°C (Wang et al, 2005a;Tang et al, 2010).…”

Section: Rapid Amplification Of Cdna Ends (Race)mentioning

confidence: 99%

“…TPP and TPS genes have been cloned from plants, bacteria, and fungi, but none have been identified in mammals. In arthropods, TPS and TPP have been cloned or deduced from genomic sequences (according to GenBank) and are known to be very important for insect development; however, there are only a few studies on TPS genes and the details of protein characteristics in insects: D. melanogaster (Chen et al, 2002), Callinectes sapidus (Chung, 2008), Locusta migratoria (Cui et al, 2009), Helicoverpa armigera (Xu et al, 2009), and Spodoptera exigua (Tang et al, 2010). Catantops pinguis (syn.…”

Section: Introductionmentioning

confidence: 99%

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Cloning and pattern of expression of trehalose-6-phosphate synthase cDNA from Catantops pinguis (Orthoptera: Catantopidae)

Tang¹,

Zheng²,

Xu³

et al. 2011

Eur. J. Entomol.

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Abstract.Trehalose is not only an important disaccharide, but also a key stress resistance factor in the development of many organisms, including plants, bacteria, fungi, and insects. To study the potential function of trehalose in development and behaviour, cDNA for a trehalose-6-phosphate synthase from Catantops pinguis (CpiTPS) was cloned and sequenced. Results revealed that the CpiTPS cDNA sequence contains an open reading frame of 2430 nucleotides encoding a protein of 809 amino acids with a predicted molecular weight of 91.13 kDa and a pI value of 6.25. Northern blot and RT-PCR analyses showed that CpiTPS mRNA expression was high in the fat body and testes, ovaries, Malpighian tubules, brain, trachea, rectum, and posterior extensor of C. pinguis. Northern blotting revealed that CpiTPS mRNA was expressed in the fat body at different developmental stages and was present at a high level in first instar larvae and adults. The results demonstrate that CpiTPS is a key gene in C. pinguis development.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 94%

Section: Rapid Amplification Of Cdna Ends (Race)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cloning and pattern of expression of trehalose-6-phosphate synthase cDNA from Catantops pinguis (Orthoptera: Catantopidae)

Tang¹,

Zheng²,

Xu³

et al. 2011

Eur. J. Entomol.

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SEQUENCING AND CHARACTERIZATION OF GLYCOGEN SYNTHASE AND GLYCOGEN PHOSPHORYLASE GENES FROM Spodoptera exigua AND ANALYSIS OF THEIR FUNCTION IN STARVATION AND EXCESSIVE SUGAR INTAKE

Tang

et al. 2012

Arch Insect Biochem Physiol

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Glycogen and trehalose are important energy source and key regulation factors in the development of many organisms' pass through energy metabolism, including bacteria, fungi, and insects. To study glycogen metabolism pathway in Spodoptera exigua, first cDNAs for glycogen synthase (SpoexGS) and glycogen phosphorylase (SpoexGP) were cloned from S. exigua. SpoexGS cDNA contains an open reading frame of 2,010 nucleotides encoding a protein of 669 amino acids with a predicted molecular mass of 76.19 kDa and a pI of 5.84. SpoexGP contains an open reading frame of 2,946 nucleotides, which encodes a protein of 841 amino acids with a predicted molecular mass of approximately 96.63 kDa and a pI of 6.03. Second, Northern blotting revealed that SpoexGS and SpoexGP mRNAs were expressed in brain, fat body, mid-gut, Malpighian tubules, spermary, and tracheae of S. exigua. Expression patterns for SpoexGS and SpoexGP mRNAs were similar in fat body, but differed in whole body at different developmental stages. The last, under starvation conditions, SpoexGS and SpoexGP transcript expression rapidly decreased with increasing starvation time. When the starvation stress was removed, SpoexGS and SpoexGP mRNA levels were lower in the groups starved for 6 and 12 h than in the 24-h starvation and control groups. Treatment with excessive sugar intake led to higher levels of SpoexGS and SpoexGP transcripts after 12 h compared to the control group. These findings provide new data on the tissue distribution, expression patterns, and potential function of glycogen synthase and glycogen phosphorylase proteins.

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Knockdown of two trehalose-6-phosphate synthases severely affects chitin metabolism gene expression in the brown planthopperNilaparvata lugens

et al. 2016

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Characterization of a trehalose-6-phosphate synthase gene from Spodoptera exigua and its function identification through RNA interference

Cited by 76 publications

References 34 publications

Cloning and pattern of expression of trehalose-6-phosphate synthase cDNA from Catantops pinguis (Orthoptera: Catantopidae)

Cloning and pattern of expression of trehalose-6-phosphate synthase cDNA from Catantops pinguis (Orthoptera: Catantopidae)

SEQUENCING AND CHARACTERIZATION OF GLYCOGEN SYNTHASE AND GLYCOGEN PHOSPHORYLASE GENES FROM Spodoptera exigua AND ANALYSIS OF THEIR FUNCTION IN STARVATION AND EXCESSIVE SUGAR INTAKE

Knockdown of two trehalose-6-phosphate synthases severely affects chitin metabolism gene expression in the brown planthopperNilaparvata lugens

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