Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants

Gross-Amat, Olivia; Guillen, Marine; Salmon, D.; Nataf, Serge; Auxenfans, Céline

doi:10.3390/ijms21186956

Cited by 13 publications

(9 citation statements)

References 41 publications

(64 reference statements)

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“…A recent report by the World Health Organization estimates that burns will cause 180,000 deaths each year. 1,2 What is more worrying is that burn survivors often suffer from varying degrees of damage to the intestinal barrier, 3 whose development is usually associated with complex factors including stress, ischemia, hypoxia, ischemia-reperfusion injury, proinflammatory cytokines, etc. [3][4][5][6] As we all know, intestinal barrier could prevent bacteria and endotoxin from translocating to other distant organs.…”

Section: Introductionmentioning

confidence: 99%

Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats

Qin¹,

Jiang²,

Yu³

et al. 2021

JIR

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Background: More and more burn survivors were suffering from varying degrees of damage to the intestinal barrier. Dexmedetomidine (Dex) was frequently used as sedative in more cases, but it was found to have repair effect on intestinal barrier dysfunction recently. This study aimed to explore the potential specific targets of Dex in intestinal barrier repair in burn rats model. Methods: Male adult SD rats were used to establish 40% TBSA III degree scald model in our study. The samples were divided into four groups: burn rats (Burn), burn rats with Dex medication (Burn-Dex), sham rats (Sham) and sham rats with Dex medication (Sham-Dex). And plasma FITC-dextran and diamine oxidase (DAO) were detected to determine the intestinal permeability. Differentially expressed proteins were further adopted to proteinprotein interaction network analysis, Gene Ontology analysis (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results: It showed that 40% TBSA III degree scald model was successfully constructed. And plasma FITC-dextran and DAO decreased significantly after Dex administration. Additionally, differentially expressed genes Psmb10, Psmb7 among the experimental groups were screened, which were significantly enriched in proteasome and other several pathways. Conclusion:The results above suggested that Q4KM35 and Q9JHW0, which are encoded by Psmb10 and Psmb7, respectively, are two possible protein targets of Dex in intestinal barrier repair.

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Section: Introductionmentioning

confidence: 99%

Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats

Qin¹,

Jiang²,

Yu³

et al. 2021

JIR

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“…Accordingly, this model is likely to also be suitable for investigating wound healing in response to mechanical, thermal or chemical injuries that damage the skin and expose the dermis. 2,32,33 Furthermore, it has been established that proliferating keratinocytes produce and release several growth factors that also promote melanocyte survival, proliferation and dendrite formation via a paracrine mechanism; hence the concept of epidermal melanin unit which expanded to also include dermal fibroblasts, Langerhans cells and endothelial cells, among others. [6][7][8][9][10]31,34,35 In the 3D-SeboSkin experiments of this study, not only the number of epidermal Melan-A+ melanocytes was preserved but also it was significantly higher than those of skin explants in SFM alone and in agreement with a previous own study in which melanocyte dendricity and proliferation were induced by SZ95 sebocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, basal keratinocytes not only survived but also proliferated with multilayer formation and migrated to cover the exposed dermis at the edge of the skin explants. Accordingly, this model is likely to also be suitable for investigating wound healing in response to mechanical, thermal or chemical injuries that damage the skin and expose the dermis 2,32,33 . Furthermore, it has been established that proliferating keratinocytes produce and release several growth factors that also promote melanocyte survival, proliferation and dendrite formation via a paracrine mechanism; hence the concept of epidermal melanin unit which expanded to also include dermal fibroblasts, Langerhans cells and endothelial cells, among others 6–10,31,34,35 .…”

Section: Discussionmentioning

confidence: 99%

Preservation of epidermal melanocyte integrity in an ex vivo co‐culture skin model with sebocytes

Abdel-Naser

Nikolakis

Zouboulis

2023

Experimental Dermatology

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A direct contact co‐culture of skin explants to SZ95 sebocytes (3D‐SeboSkin) has been shown to preserve the integrity of epidermal keratinocytes and dermis. In this study, the properties of epidermal melanocytes were evaluated in the same 3D SeboSkin ex vivo model. Skin explants (n = 6) were maintained in the 3D‐SeboSkin model, in direct contact to fibroblasts and alone in serum‐free medium (SFM). Histopathological, immunohistochemical, apoptosis and oil red staining evaluations were performed at Days 0 and 6 of incubation. Results revealed preservation and prominent proliferation of basal keratinocytes of the skin explants in addition to preservation of dermal collagen and vasculature at Day 6 in the 3D‐SeboSkin culture model and to a lesser extent in co‐culture with fibroblasts but not in SFM alone. Melan‐A+/Ki67‐ epidermal melanocytes remained attached to the dermis even at sites of epidermal detachment in the three skin explant models tested. However, the number of epidermal melanocytes was significantly conserved in 3D‐SeboSkin cultures in comparison with skin explants in SFM (p < 0.05), whereas no difference was found in comparison with the co‐culture with fibroblasts. Few DAPI/TUNEL+ apoptotic melanocytes could mostly be observed in SFM‐incubated skin explants. Furthermore, only SZ95 sebocytes in contact to skin explants in 3D‐SeboSkin exhibited increased lipogenesis with accumulation of abundant lipid droplets. These results denote that the 3D‐SeboSkin model yielded significant preservation of epidermal melanocytes and hence it is optimal for ex vivo studies of abnormalities of skin pigmentation, melanocyte neoplasms and effects of different hormones, cytokines, carcinogens and various therapeutics in a pattern that recapitulates the in vivo environment.

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“…Vostálová et al [1] 2019 -After 8 days, TNFα induce type 1 collagen degradation through MMP1 Ågren et al [2] 2015 -After 9 days, TNFα and IL1 α induce arrest of 18 s transcription Neil et al [3] 2020 -HLA II-positive cells exist in burned HSEs revealing a real level of skin immunocompetence even when the skin is explanted Gross-Amat et al [4] 2020 How to prolong the duration and quality of HSE tissues in culture? -Proliferating keratinocytes exist in HSEs up to 75 days Frade et al [5] 2015 -Culture medium pH should be (>5.5), isotonic with skin cells and should contain 0.5 g L-1 of glucose.…”

Section: Review Methodologymentioning

confidence: 99%

Emergence of New Concepts in Skin Physiopathology through the Use of in vitro Human Skin Explants Models

Cousin,

Misery,

de Vries

et al. 2023

Dermatology

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Background: This review summarizes uses and new applications for dermatological research of in vitro culture models of human skin explants (HSEs). In the last decade, many innovations have appeared in the literature and an exponential number of studies have been recorded in various fields of application such as process culture engineering, stem cell extractions methodology, or cell-to-cell interaction studies under physiological and pathological conditions, wound-healing, and inflammation. Most studies also concerned pharmacology, cosmetology, and photobiology. However, these topics will not be considered in our review. Summary: A better understanding of the mechanisms driving intercellular relationships, at work in the maintenance of 3D tissue architectures has led to the improvement of cell culture techniques. Many papers have focused on the physiological ways that govern in vitro tissue maintenance of HSEs. The analysis of the necessary mechanical stress, intercellular and cell-matrix interactions, allows the maintenance and prolonged use of HSEs in culture for up to 15 days, regardless of the great variability of study protocols from one laboratory to another and in accordance with the objectives set. Because of their close similarities to fresh skin, HSEs are increasingly used to study skin barrier repair and wound healing physiology. Easy to use in co-culture, this model allows a better understanding of the connections and interactions between the peripheral nervous system, the skin and the immune system. The development of the concept of an integrated neuro-immuno-cutaneous system at work in skin physiology and pathology highlighted by this article represents one of the new technical challenges in the field of in vitro culture of HSE. This review of the literature also reveals the importance of using such models in pathology. As sources of stem cells, HSEs are the basis for the development of new tissue engineering models such as organoids or optical clearing tissues technology. This study identifies the main advances and cross-cutting issues in the use of HSE.

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Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants

Cited by 13 publications

References 41 publications

Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats

Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats

Preservation of epidermal melanocyte integrity in an ex vivo co‐culture skin model with sebocytes

Emergence of New Concepts in Skin Physiopathology through the Use of in vitro Human Skin Explants Models

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