Characterization of a T7-Like Lytic Bacteriophage (φSG-JL2) of
            <i>Salmonella enterica</i>
            Serovar Gallinarum Biovar Gallinarum

Kwon, Hyuk‐Joon; Cho, Sun-Hee; Kim, Tae‐Eun; Won, Yong-Jin; Jeong, Ji-Hoon; Kim, Jae Hong; Yoo, Han Sang; Park, Taesung; Kim, Sun-Joong

doi:10.1128/aem.01088-08

Cited by 26 publications

(14 citation statements)

References 81 publications

(90 reference statements)

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“…All three bacteriophages belong to the Caudovirales order, and their genomes do not show homology with any known gene involved in virulence, as determined after sequencing of their genomes (data not shown). Moreover, these bacteriophages are highly stable, as confirmed by the observation that their concentrations after a 30-min incubation at pH 2 decreased by a maximum of 3.4 log 10 PFU/ml (data not shown), which is better than the values reported by other authors (17,19). These results suggest that the three bacteriophages are able to resist passage through the low-pH environment of the stomach.…”

Section: Discussionsupporting

confidence: 70%

Significance of the Bacteriophage Treatment Schedule in Reducing Salmonella Colonization of Poultry

Bardina

Spricigo

Cortés

et al. 2012

Appl Environ Microbiol

110

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bSalmonella remains the major cause of food-borne diseases worldwide, with chickens known to be the main reservoir for this zoonotic pathogen. Among the many approaches to reducing Salmonella colonization of broilers, bacteriophage offers several advantages. In this study, three bacteriophages (UAB_Phi20, UAB_Phi78, and UAB_Phi87) obtained from our collection that exhibited a broad host range against Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium were characterized with respect to morphology, genome size, and restriction patterns. A cocktail composed of the three bacteriophages was more effective in promoting the lysis of S. Enteritidis and S. Typhimurium cultures than any of the three bacteriophages alone. In addition, the cocktail was able to lyse the Salmonella enterica serovars Virchow, Hadar, and Infantis. The effectiveness of the bacteriophage cocktail in reducing the concentration of S. Typhimurium was tested in two animal models using different treatment schedules. In the mouse model, 50% survival was obtained when the cocktail was administered simultaneously with bacterial infection and again at 6, 24, and 30 h postinfection. Likewise, in the White Leghorn chicken specific-pathogen-free (SPF) model, the best results, defined as a reduction of Salmonella concentration in the chicken cecum, were obtained when the bacteriophage cocktail was administered 1 day before or just after bacterial infection and then again on different days postinfection. Our results show that frequent treatment of the chickens with bacteriophage, and especially prior to colonization of the intestinal tract by Salmonella, is required to achieve effective bacterial reduction over time.

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Section: Discussionsupporting

confidence: 70%

Significance of the Bacteriophage Treatment Schedule in Reducing Salmonella Colonization of Poultry

Bardina

Spricigo

Cortés

et al. 2012

Appl Environ Microbiol

110

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“…One-step growth analysis of phage SPN9CC revealed a longer latency period and a smaller burst size than those of other lytic phages, such as T7-like Podoviridae family phages (Fig. 5A) (59)(60)(61), suggesting that lysogen formation during phage infection may affect the host cell lysis activity of phage SPN9CC. A high frequency of observed mutants insensitive to this phage during a bacterial challenge test also supports this (see Fig.…”

Section: Discussionmentioning

confidence: 97%

Genomic Investigation of Lysogen Formation and Host Lysis Systems of the Salmonella Temperate Bacteriophage SPN9CC

Shin

Lee

Yoon

et al. 2014

Appl Environ Microbiol

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cTo understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and forms clear plaques with cloudy centers due to lysogen formation. Phylogenetic analysis of phage major capsid proteins revealed that this phage is a member of the lysogen-forming P22-like phage group. However, comparative genomic analysis of SPN9CC with P22-like phages indicated that their lysogeny control regions and host cell lysis gene clusters show very low levels of identity, suggesting that lysogen formation and host cell lysis mechanisms may be diverse among phages in this group. Analysis of the expression of SPN9CC host cell lysis genes encoding holin, endolysin, and Rz/Rz1-like proteins individually or in combinations in S. Typhimurium and Escherichia coli hosts revealed that collaboration of these lysis proteins is important for the lysis of both hosts and that holin is a key protein. To further investigate the role of the lysogeny control region in phage SPN9CC, a ⌬cI mutant (SPN9CCM) of phage SPN9CC was constructed. The mutant does not produce a cloudy center in the plaques, suggesting that this mutant phage is virulent and no longer temperate. Subsequent comparative one-step growth analysis and challenge assays revealed that SPN9CCM has shorter eclipse/latency periods and a larger burst size, as well as higher host cell lysis activity, than SPN9CC. The present work indicates the possibility of engineering temperate phages as promising biocontrol agents similar to virulent phages.

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“…Two Salmonella -specifi c phages belong to this genus S . Gallinarum phage φSG-JL2 [ 78 ] and S . Typhi phage Vi06 [ 19 ].…”

Section: T7likevirusmentioning

confidence: 99%

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Switt

Sulakvelidze

Wiedmann

et al. 2014

Methods in Molecular Biology

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Since this book was originally published in 2007 there has been a significant increase in the number of Salmonella bacteriophages, particularly lytic virus, and Salmonella strains which have been fully sequenced. In addition, new insights into phage taxonomy have resulted in new phage genera, some of which have been recognized by the International Committee of Taxonomy of Viruses (ICTV). The properties of each of these genera are discussed, along with the role of phage as agents of genetic exchange, as therapeutic agents, and their involvement in phage typing.

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Characterization of a T7-Like Lytic Bacteriophage (φSG-JL2) of Salmonella enterica Serovar Gallinarum Biovar Gallinarum

Cited by 26 publications

References 81 publications

Significance of the Bacteriophage Treatment Schedule in Reducing Salmonella Colonization of Poultry

Significance of the Bacteriophage Treatment Schedule in Reducing Salmonella Colonization of Poultry

Genomic Investigation of Lysogen Formation and Host Lysis Systems of the Salmonella Temperate Bacteriophage SPN9CC

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

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