Characterization of a T cell-derived lymphokine that acts synergistically with IL 3 on the growth of murine mast cells and is identical with IL4

Schmitt, Edgar; Faßbender, Birgit; Beyreuther, Konrad; Spaeth, E.; Schwarzkopf, R.; Rüde, Erwin

doi:10.1016/s0171-2985(87)80014-1

Cited by 31 publications

(10 citation statements)

References 23 publications

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“…Therefore, mast cell mediators such as cytokines may participate in the regulation of the number of mast cells in the tissues. It has been reported that mast cells differentiate and proliferate under the influence of an array of cytokines and growth factors, such as IL-3, IL-4 and IL-10 derived from T cells and possibly from mast cells [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…In man, however, a greater number of intestinal mast cells has been reported in IBD patients in comparison with healthy subjects [16,17] and mast cells may play a functional role in the inflammatory process of IBD [17] and its associated alterations of intestinal ion transport [16]. Several factors have been shown to promote and stimulate the development of mast cell hyperplasia such as T cells [18], stem cell factor secreted by fibroblasts and other stromal cells, epithelial and endothelial cells [19], interleukin-3 (IL-3), IL-4 and IL-10 produced by T cells and mast cells [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Development and Sequels of Intestinal Inflammation in Nematode-Infected Rats: Role of Mast Cells and Capsaicin-Sensitive Afferents

Gay¹,

Fioramonti²,

Garcia‐Villar³

et al. 2000

Neuroimmunomodulation

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Objectives: To determine whether intestinal mast cells and capsaicin-sensitive afferent nerves are involved in the development and sequels of Nippostrongylus brasiliensis-induced intestinal inflammation in rats. Methods: Two series of experiments were performed. In the first series, six groups of 8 rats were used to study the effects of mast cell stabilization by ketotifen. In the second series, six groups of 6 rats were used to study the effects of gut extrinsic sensory neuron depletion by capsaicin. For each series, four groups of rats were infected with N. brasiliensis and two groups were not infected. Results: Infection with N. brasiliensis resulted in an increase of myeloperoxidase (MPO) activity and mast cell numbers at day 12 postinfection; MPO returned to preinfection levels by day 35 while mast cell numbers remained elevated at that time. In ketotifen-treated infected rats, the increase of MPO at day 12 was less pronounced, but MPO activity remained elevated and mast cell numbers were increased at day 35. In capsaicin-treated infected rats, the MPO increase at day 12 was augmented, and MPO was still not returned to preinfection values by day 35; in contrast, the increase of mast cell numbers at days 12 and 35 was not modified by afferent nerve depletion. Conclusion: Mast cell stabilization decreased jejunal inflammation during the acute stage (day 12), but prolonged the inflammatory process until at least day 35 postinfection. The data also confirmed the protective role of gut extrinsic sensory neurons against intestinal inflammation in a model of nematode infection and revealed that these afferent nerves do not seem crucial for the development of nematode-induced hypermastocytosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Sequels of Intestinal Inflammation in Nematode-Infected Rats: Role of Mast Cells and Capsaicin-Sensitive Afferents

Gay¹,

Fioramonti²,

Garcia‐Villar³

et al. 2000

Neuroimmunomodulation

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“…It was demonstrated that mast cells proliferate in response to special growth factors [4,5], and their phenotypic features and antigenic determinants also depend on the specific microenvironment [25]. There fore, the start of a small portion of cells to express the MAb-detectable antigen ( fig.…”

Section: Discussionmentioning

confidence: 99%

“…Now it is widely accepted that mast cells differentiate from multipoten tial hematopoietic stem cells in the bone marrow [ 1 -3]. The differentiation of mast cell precursors into mature mast cells was demonstrated to be dependent on the presence of IL3 and/or IL4 [ 1,4,5], whereas the phenot ypic feature of the mature mast cells seems to be strongly influenced by factors of the immediate micro environment [6]. Changing the microenvironment of mast cells, one can induce the transdifferentiation of connective tissue mast cells (CTMC) into mucosa mast cells ( M MC) or vice versa [1,7].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Mast Cell Differentiation in vivo by Use of Monoclonal Antibodies

Odarjuk

Rossow

Karawajew

1989

Int Arch Allergy Immunol

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In the present study mast cell differentiation/maturation was studied in vivo after depletion of mature mast cells from the peritoneal cavity by injection of distilled water. The reconstituting cell population was characterized by use of different staining methods. Additionally, the monoclonal antibody (MAb) IWF F2, which recognizes a membrane antigen of rat mast cells, was used to follow up mast cell differentiation/maturation in the course of the experiment. The antigen expression was studied both by immunofluorescence of antigen-bearing cells and by MAb inhibition of compound 48/80-stimulated histamine release from mast cells. In the course of the experiment the amount of antigen-positive cells increased continuously from less than 5% to control level (1st and 22nd days, respectively). The expression of the membrane antigen detectable by the MAb precedes the appearance of cytochemically identifiable mast cells for several days. The mast cells mature morphologically and functionally as indicated by increasing size, histamine content and MAb inhibition of compound 48/80-stimulated histamine release. The results obtained suggest the MAb IWF F2 to be a useful methodical tool for additional characterization of mast cell differentiation/maturation processes.

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“…Alternatively, a small number of Ia' BMMQ might stimulate antigen-specifically few responder T clone cells, which would then increase the Ia' BMMQ fraction by secreting IFN-y or IL4. ST2K.9 T cells were reported to belong to the Th2 group of T cells [29] producing IL4 [30], which was recently shown to possess Ia-inducing activity for MQ [31, 321. An influence by a few contaminating T cells within a BM cell preparation on the expression of Ia molecules on BMMQ via secretion of IFN-y was suggested by the experiments of Puri et al [33].…”

Section: Discussionmentioning

confidence: 99%

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

et al. 1988

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The antigen-mediated activation of a number of T cell clones by bone marrow (BM) cells cultivated in the presence of various colony-stimulating factor (CSF) preparations was investigated. BM macrophages (BMM phi) grown in L929 cell supernatant as a crude source of macrophage colony-stimulating factor (M-CSF) as well as BM cells propagated in the presence of recombinant M-CSF exhibited transient antigen presentation potential to some T cell clones, being maximal on day 7 and having declined to a low level by day 19 of in vitro culture. Treatment of these long-term-cultivated BMM phi populations with recombinant interferon-gamma (IFN-gamma) resulted in predominant antigen presentation capacity. In contrast, BM cells differentiated in the presence of recombinant granulocyte (G)M-CSF developed highly efficient accessory cell function to all T cell clones examined. This function became apparent earlier, was retained during the time period tested (up to day 19 of continuous culture) and did not require prior stimulation by IFN-gamma. The functionally competent cells were shown to belong to the monocyte/macrophage lineage. These findings are consistent with the demonstration of substantial levels of major histocompatibility complex (MHC) class II molecules synthesized by GM-CSF-cultured BM cells in the absence of exogenous IFN-gamma. In contrast, M-CSF grown BM cells synthesized only minute amounts of Ia antigens unless they were stimulated by IFN-gamma. Because GM-CSF-cultivated BM cells proved clearly superior to M-CSF-grown and IFN-gamma-activated BM cells with respect to antigen-presenting capacity but exhibited lower levels of MHC class II molecules, other properties acting in addition to surface Ia antigens might be responsible for their pronounced T cell accessory function.

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Characterization of a T cell-derived lymphokine that acts synergistically with IL 3 on the growth of murine mast cells and is identical with IL4

Cited by 31 publications

References 23 publications

Development and Sequels of Intestinal Inflammation in Nematode-Infected Rats: Role of Mast Cells and Capsaicin-Sensitive Afferents

Development and Sequels of Intestinal Inflammation in Nematode-Infected Rats: Role of Mast Cells and Capsaicin-Sensitive Afferents

Investigation of Mast Cell Differentiation in vivo by Use of Monoclonal Antibodies

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

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