Characterization of a subfamily of related winged helix genes, XFD-12/12′/12″ (XFLIP), during Xenopus embryogenesis

Sölter, Marion; Köster, Manfred; Hollemann, Thomas; Brey, Andreas; Pieler, Tomas; Knöchel, Walter

doi:10.1016/s0925-4773(99)00195-1

Cited by 30 publications

(29 citation statements)

References 21 publications

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“…This more restricted expression pattern suggests that the two flotillin1 paralogues may be under the control of different regulatory regions in the genome. Where different paralogues have been identified in Xenopus laevis, similar small differences in expression patterns have been reported (e.g., for FoxD5: Solter et al, 1999;Fetka et al, 2000;Sullivan et al, 2001). …”

Section: Resultssupporting

confidence: 69%

Xenopus flotillin1, a novel gene highly expressed in the dorsal nervous system

Pandur

Dirksen

Moore

et al. 2004

Developmental Dynamics

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The two paralogues of the Xenopus flotillin1 gene (flotillin1A and flotillin1B), which encodes a putative membraneassociated protein, were cloned from egg, cleavage, and tadpole cDNA libraries. Both mRNAs are present during oogenesis and cleavage stages. After the onset of zygotic transcription, flotillin1 transcripts are first expressed throughout the embryonic ectoderm and become enhanced in the presumptive neural ectoderm as the neural plate forms. As the neural tube forms and differentiates, flotillin1 transcripts become enriched in the dorsal half, with particularly high expression in dorsal primary neurons. At early tail bud stages, there is additional expression in the paraxial mesoderm. At late tail bud stages, flotillin1A is expressed in branchial arch mesenchyme, the overlying branchial ectoderm and in dorsal somitic mesoderm, whereas flotillin1B expression is more restricted in the dorsal neural tube and head sensory structures. This report is the first comprehensive developmental description in any animal of the expression pattern of this gene, whose protein product in several systems plays important roles in signal transduction events. Developmental Dynamics 231:881-887, 2004.

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Section: Resultssupporting

confidence: 69%

Xenopus flotillin1, a novel gene highly expressed in the dorsal nervous system

Pandur

Dirksen

Moore

et al. 2004

Developmental Dynamics

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“…Constructs used were: Xdsh-∆PDZ/D2 (Xdsh-D2) , Xdd1 (Sokol, 1996), XStbm (Darken et al, 2002), Xash-3 (Zimmerman et al, 1993), Xpax-3 (Bang et al, 1999, Xnetrin (de la Torre et al, 1997), Sox-2 (Mizuseki et al, 1998) and Xfd12 (Solter et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

“…During gastrula stages, Xfd12 is expressed in ventral/medial neural precursors (Solter et al, 1999). During early gastrulation, robust convergent extension reorganizes the neural plate , and these movements are very closely paralleled by the expression pattern of Xfd12 (Fetka et al, 2000).…”

Section: Neural Tube Closure Requires Xdsh Function In the Midline Bmentioning

confidence: 98%

Neural tube closure requires Dishevelled-dependent convergent extension of the midline

2002

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In Xenopus, Dishevelled (Xdsh) signaling is required for both neural tube closure and neural convergent extension, but the connection between these two morphogenetic processes remains unclear. Indeed normal neurulation requires several different cell polarity decisions, any of which may require Xdsh signaling. In this paper we address two issues: (1) which aspects of normal neurulation require Xdsh function; and (2) what role convergent extension plays in the closure of the neural tube. We show that Xdsh signaling is not required for neural fold elevation, medial movement or fusion. Disruption of Xdsh signaling therefore provides a specific tool for uncoupling convergent extension from other processes of neurulation. Using disruption of Xdsh signaling, we demonstrate that convergent extension is crucial to tube closure. Targeted injection revealed that Xdsh function was required specifically in the midline for normal neural tube closure. We suggest that the inherent movement of the neural folds can accomplish only a finite amount of medial progress and that convergent extension of the midline is necessary to reduce the distance between the nascent neural folds, allowing them to meet and fuse. Similar results with Xenopus strabismus implicate the planar cell polarity (PCP) signaling cascade in neural convergent extension and tube closure. Together, these data demonstrate that PCP-mediated convergent extension movements are crucial to proper vertebrate neurulation. Movies available on-line

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“…Geminin, Foxd4l1, Neogenin, Sox3, Zic1 and Zic3 probes were prepared via PCR amplification of published coding regions from cDNA, adding a bacteriophage T7 promoter to the 3Ј end: xGeminin (Kroll et al, 1998;McGarry and Kirschner, 1998), xFoxd4l1 (Sölter et al, 1999;Sullivan et al, 2001), xNeogenin (Anderson and Holt, 2002), xSox3 (Denny et al, 1992;Penzel et al, 1997), xZic1 (Kuo et al, 1998;Mizuseki et al, 1998;Nakata et al, 1998) and xZic3 (Nakata et al, 1997). Erf-and Etv3-coding sequences (Klein et al, 2002) were cloned into pBluescript II SK-.…”

Section: Whole-mount In Situ Hybridization (Wish)mentioning

confidence: 99%

ERF and ETV3L are retinoic acid-inducible repressors required for primary neurogenesis

et al. 2013

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SUMMARYCells in the developing neural tissue demonstrate an exquisite balance between proliferation and differentiation. Retinoic acid (RA) is required for neuronal differentiation by promoting expression of proneural and neurogenic genes. We show that RA acts early in the neurogenic pathway by inhibiting expression of neural progenitor markers Geminin and Foxd4l1, thereby promoting differentiation. Our screen for RA target genes in early Xenopus development identified Ets2 Repressor Factor (Erf) and the closely related ETS repressors Etv3 and Etv3-like (Etv3l). Erf and Etv3l are RA responsive and inhibit the action of ETS genes downstream of FGF signaling, placing them at the intersection of RA and growth factor signaling. We hypothesized that RA regulates primary neurogenesis by inducing Erf and Etv3l to antagonize proliferative signals. Loss-of-function analysis showed that Erf and Etv3l are required to inhibit proliferation of neural progenitors to allow differentiation, whereas overexpression of Erf led to an increase in the number of primary neurons. Therefore, these RA-induced ETS repressors are key components of the proliferation-differentiation switch during primary neurogenesis in vivo.

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Characterization of a subfamily of related winged helix genes, XFD-12/12′/12″ (XFLIP), during Xenopus embryogenesis

Cited by 30 publications

References 21 publications

Xenopus flotillin1, a novel gene highly expressed in the dorsal nervous system

Xenopus flotillin1, a novel gene highly expressed in the dorsal nervous system

Neural tube closure requires Dishevelled-dependent convergent extension of the midline

ERF and ETV3L are retinoic acid-inducible repressors required for primary neurogenesis

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