Characterization of a site contaminated by chlorinated ethenes and ethanes using multi-analysis

Kaown, Dugin; Jun, Seong-Chun; Kim, Rak-Hyeon; Shin, Woosik; Lee, Kang‐Kun

doi:10.1007/s12665-016-5536-2

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…Increase in abundance of an unclassified taxon associated with Deltaproteobacteria also occurred in the later phase of incubation (Figure 5). Indeed, Deltaproteobacteria are often associated with key dechlorinators in contaminated groundwater (Kotik et al, 2013; Adetutu et al, 2015; Kaown et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater

Hermon

Hellal

Denonfoux³

et al. 2019

Front. Microbiol.

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Microcosm experiments with CE-contaminated groundwater from a former industrial site were set-up to evaluate the relationships between biological CE dissipation, dehalogenase genes abundance and bacterial genera diversity. Impact of high concentrations of PCE on organohalide respiration was also evaluated. Complete or partial dechlorination of PCE, TCE, cis -DCE and VC was observed independently of the addition of a reducing agent (Na 2 S) or an electron donor (acetate). The addition of either 10 or 100 μM PCE had no effect on organohalide respiration. qPCR analysis of reductive dehalogenases genes ( pceA, tceA, vcrA , and bvcA ) indicated that the version of pceA gene found in the genus Dehalococcoides [hereafter named pceA (Dhc)] and vcrA gene increased in abundance by one order of magnitude during the first 10 days of incubation. The version of the pceA gene found, among others, in the genus Dehalobacter, Sulfurospirillum, Desulfuromonas , and Geobacter [hereafter named pceA (Dhb)] and bvcA gene showed very low abundance. The tceA gene was not detected throughout the experiment. The proportion of pceA (Dhc) or vcrA genes relative to the universal 16S ribosomal RNA (16S rRNA) gene increased by up to 6-fold upon completion of cis- DCE dissipation. Sequencing of 16S rRNA amplicons indicated that the abundance of Operational Taxonomic Units (OTUs) affiliated to dehalogenating genera Dehalococcoides, Sulfurospirillum , and Geobacter represented more than 20% sequence abundance in the microcosms. Among organohalide respiration associated genera, only abundance of Dehalococcoides spp. increased up to fourfold upon complete dissipation of PCE and cis -DCE, suggesting a major implication of Dehalococcoides in CEs organohalide respiration. The relative abundance of pceA and vcrA genes correlated with the occurrence of Dehalococcoides and with dissipation extent of PCE, cis -DCE and CV. A new type of dehalogenating Dehalococcoides sp. phylotype affiliated to the Pinellas group, and suggested to contain both pceA (Dhc) and vcrA genes, may be involved in organohalide respiration of CEs in groundwater of the study site. Overall, the results demonstrate in situ dechlorination potential of CE in the plume, and suggest that taxonomic and functional biomarkers in laboratory microcosms of contaminated groundwater following pollutant exposure can ...

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Section: Discussionmentioning

confidence: 99%

Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater

Hermon

Hellal

Denonfoux³

et al. 2019

Front. Microbiol.

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“…A northeastern American industrial area was polluted with 38 μM TCA and 8 μM TCE ( Grostern and Edwards, 2006 ). Kaown et al (2016) found that both chlorinated ethenes and ethanes were present in the industrial area of Asan, Korea, and the levels of monitored TCE and TCA ranged from 0.004 to 5.8 mg/L and from non-detected to 1.8 mg/L, respectively. Though initially was not suggested as an unidentified contamination ( Halden and Paull, 2005 ), TCC was monitored at a value of 6750 ng/L with a frequency of 68% in United States water resources.…”

Section: Introductionmentioning

confidence: 97%

“…These sites were also possibly contaminated with TCE and TCA ( Zhao J.L. et al, 2010 ; Kaown et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of 1,1,1-Trichloroethane and Triclocarban on Reductive Dechlorination of Trichloroethene in a TCE-Reducing Culture

et al. 2017

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Chlorinated compounds were generally present in the environment due to widespread use in the industry. A short-term study was performed to evaluate the effects of 1,1,1- trichloroethane (TCA) and triclocarban (TCC) on trichloroethene (TCE) removal in a reactor fed with lactate as the sole electron donor. Both TCA and TCC inhibited TCE reduction, but the TCC had a more pronounced effect compared to TCA. The TCE-reducing culture, which had never been exposed to TCA before, reductively dechlorinated TCA to 1,1-dichloroethane (DCA). Below 15 μM, TCA had little effect on the transformation of TCE to cis-dichloroethene (DCE); however, the reduction of cis-DCE and vinyl chloride (VC) were more sensitive to TCA, and ethene production was completely inhibited when the concentration of TCA was above 15 μM. In cultures amended with TCC, the reduction of TCE was severely affected, even at concentrations as low as 0.3 μM; all the cultures stalled at VC, and no ethene was detected. The cultures that fully transformed TCE to ethene contained 5.2–8.1% Dehalococcoides. Geobacter and Desulfovibrio, the bacteria capable of partially reducing TCE to DCE, were detected in all cultures, but both represented a larger proportion of the community in TCC-amended cultures. All cultures were dominated by Clostridium_sensu_stricto_7, a genus that belongs to Firmicutes with proportions ranging from 40.9% (in a high TCC (15 μM) culture) to 88.2%. Methanobacteria was detected at levels of 1.1–12.7%, except in cultures added with 15 and 30 μM TCA, in which they only accounted for ∼0.4%. This study implies further environmental factors needed to be considered in the successful bioremediation of TCE in contaminated sites.

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“…In this context, compound-specific isotope analysis (CSIA) can directly evidence in situ biodegradation of CEs, and can be coupled to diversity data. In particular, CSIA can help to evaluate pollutant degradation with respect to non-degradative dissipative processes, including sorption or dilution (e.g., Carreón-Diazconti et al, 2009;Courbet et al, 2011;Hunkeler, 2016;Kaown et al, 2016;Kuntze et al, 2020;Thullner et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…It is promising to couple these approaches to better evaluate and predict the degradation of chlorinated solvents by OHR in situ (Carréon-Diazconti et al, 2009;Courbet et al, 2011;Hunkeler et al, 2011;Hermon et al, 2018;Imfeld et al, 2011;Kaown et al, 2016). This can be extended by specific follow-up of OHRB biomarkers selected for their specificity and relevance to identified questions (Maphosa et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Chlorinated ethene biodegradation and associated bacterial taxa in multi-polluted groundwater: Insights from biomolecular markers and stable isotope analysis

Hellal

Joulian

Urien³

et al. 2021

Science of The Total Environment

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Characterization of a site contaminated by chlorinated ethenes and ethanes using multi-analysis

Cited by 10 publications

References 37 publications

Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater

Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater

Effects of 1,1,1-Trichloroethane and Triclocarban on Reductive Dechlorination of Trichloroethene in a TCE-Reducing Culture

Chlorinated ethene biodegradation and associated bacterial taxa in multi-polluted groundwater: Insights from biomolecular markers and stable isotope analysis

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