Characterization of a single SA7-like VA RNA gene in subgroup F adenoviruses

Kidd, Alistair H.; Tiemessen, Caroline T.

doi:10.1099/0022-1317-74-8-1621

Cited by 5 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The importance of VA RNA function in adenovirus replication is reflected in the fact that all known adenovirus genomes have at least one, and usually two VA RNA genes (Larsson et al, 1986 ;Kidd & Tiemessen, 1993 ;Ma & Mathews, 1993). Thus it was anticipated that OAV would also have a VA RNA gene.…”

Section: Discussionmentioning

confidence: 99%

Ovine adenovirus (OAV287) lacks a virus-associated RNA gene.

Venktesh

Watt

et al. 1998

Journal of General Virology

View full text Add to dashboard Cite

Ovine adenovirus OAV287 (OAV) is the prototype of a virus group which is phylogenetically distinct from the mastadenoviruses and aviadenoviruses. The genome arrangement of OAV showed that virusassociated (VA) RNA genes were not located between the reading frames for p52/55K and terminal protein as these overlapped. To determine whether VA genes were located elsewhere, several approaches were used. Nuclear extracts containing RNA polymerase III activity were used to transcribe OAV genome fragments in vitro. A product of " 120 bp was produced from two widely separated coding regions of the genome. However, when these were subcloned and used as radiolabelled probes to analyse RNA from OAV-infected cells, no VA-like RNA was detected, although late mRNAs that were transcribed from the regions were identified. In

show abstract

Section: Discussionmentioning

confidence: 99%

Ovine adenovirus (OAV287) lacks a virus-associated RNA gene.

Venktesh

Watt

et al. 1998

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The test used in this study is based on differentiation between the VA RNA gene regions of the different human adenovirus subgenera by PCR. Adenoviruses of subgenera A, B:2, and F have one VA RNA gene (23,24,31) and yield PCR products ranging in size from 240 to 290 bp, whereas representative adenoviruses of subgenera B:1, C, D, and E, which have two VA RNA genes rather than one, yield products from 410 to 520 bp ( Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…As differentiation between subgenera B:1, C, D, and E on this basis is not reliable, we adopted a fingerprinting approach based on the presence of two similar genes. All VA RNA genes have promoter element B regions, and these usually include a TCGA motif (24,31), which in Ad2 is thought to be important for interaction of the promoter with transcription factor TFIIIC (6). It is possible to fingerprint adenoviruses belonging to different subgenera by treating the amplified VA RNA region product with TaqI, which recognizes the TCGA motif.…”

Section: Resultsmentioning

confidence: 99%

“…Second, one monkey adenovirus strain gave a PCR product matching the size of those from subgenus F adenoviruses. As this product was not digested by SfuI and AvaI in combination, the strain was mistakenly identified as belonging to subgenus F. A high level of DNA sequence identity between the VA RNA regions of subgenus F adenoviruses and two strains of monkey adenovirus (of types SAV16 and SAV19) has been noted previously (23,24).…”

Section: Fig 4 Comparison Of Eight Commonmentioning

confidence: 87%

“…Primers. PCR products from amplification of the VA RNA regions of several adenovirus serotypes were sequenced by using previously described primers (24). These sequences were then used to identify adenovirus genus-specific primer binding sequences situated immediately upstream and downstream of each VA RNA gene region (23).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Rapid subgenus identification of human adenovirus isolates by a general PCR

Kidd¹,

Jönsson²,

Garwicz³

et al. 1996

J Clin Microbiol

View full text Add to dashboard Cite

In most clinical situations involving adenovirus infection, subgenus (subgroup) identification of an adenovirus isolate is as informative as a finer identification by serotype. A PCR method which allows the identification of human adenovirus isolates as members of subgenera A, B:1, B:2, C, D, E, or F is described. It is based on a simple (nonnested) PCR using primers which bind to regions immediately flanking the VA RNA-encoding regions of human adenovirus genomes. The PCR allows amplification of DNA from all 49 human adenovirus prototype strains so far described. Since there are differences in the lengths of the VA RNA-encoding regions in adenoviruses of different subgenera, it is possible to differentiate some subgenera according to the size of the PCR product determined by electrophoresis. This forms the basis of an initial broad categorization of isolates as belonging to either (i) subgenus B:1, C, D, or E or (ii) subgenus A, B:2, or F. Subgenus identification is completed by a one-step restriction enzyme digestion and gel electrophoresis. The method was assessed by blind subgenus identification of 200 miscellaneous primate adenovirus isolates prepared by the reference laboratory at Bilthoven, The Netherlands. Identification at the subgenus level by PCR correlated 91.5% with the results of serotyping. A further 5.5% of isolates were correctly identified as belonging to one of two specified subgenera. Six of the 200 identifications (3%) were unsuccessful for various reasons, including weak PCR products, intermediate strains, and mistaken primate host. The method should serve as a rapid means of confirming adenovirus cytopathic effects in laboratories performing virus culture, with simultaneous subgenus identification of the isolate. It will also have relevance as an aid to conventional serotyping for epidemiological purposes, since for all adenoviruses except those belonging to subgenus D, neutralization tests need only involve a maximum of four type-specific antisera.

show abstract