Characterization of a second bovine rotavirus serotype

Abstract

SummaryBovine rotavirus (BRV) V 1005 was characterized by two-way crossneutralization tests as a second serotype of BRV. Virions and inner shell particles of 65 nm and 55 nm diameter respectively, and empty capsids of 65 nm and 55 nm diameter were separated by density gradient centrifugation. Three polypeptides of molecular weight 60,000, 36,000 and 28,000 (minor protein) could be identified in the outer shell of virions and in the larger empty capsids. Inner shell particles contained three polypeptides of molecular weight 105,000, 83,000 and 43,000. Both sizes of empty eapsids showed two polypeptides of molecular weight 75,000 and 55,000 not found in virions. Pulse-labelling of infected cells revealed eight major and three minor intracellular viral polypeptides. Viral polypeptide synthesis started at about 6 hours p.i. and correlated in time with double-stranded RNA synthesis. As soon as viral polypeptide synthesis was detectable, newly synthesized virM polypeptides were incorporated into intracellular viral partieles. Radioactive viral polypeptides appeared without a longer lag period in extraeellular viruses from 6 hours p.i. onwards.

Use of Bovine Milk Concentrate Containing Antibody to Rotavirus to Treat Rotavirus Gastroenteritis in Infants

“…Daily stool samples weretaken from concentrate-treated and control infants after hospitalization for rotavirus gastroenteritis. Tworandom stool samples from each of 55 control infants and from each of 22 infants treated with concentrate B (neutralization titer, 1:1,1(0) were checked for the presence of bovine milk immunoglobulins by the Ouchterlony test and for neutralizing activity against bovine rotavirus VlOO5, which is serologically unrelated to human rotavirus [31]. Concentrate B-treated infants had bovine immunoglobulins in 21 (48010) of the 44 stool samples examined and in 11 (25010) stool samples, neutralizing activities, with a mean neutralization titer of 1:8 (mean of neutralizing stool samples only).…”

“…A series of twofold dilutions, starting with a 1:5 dilution, of stool extracts (10070 stool suspension in PBS), was used for neutralization tests, according to Gerna et al [29]. Bovine rotavirus vioos [30,31], human rotavirus Wa (serotype 1) [32], and simian rotavirus SA-ll [32] were used to measure rotavirus-neutralizing activity.…”

Presence of three P types (VP4 serotypes) and two G types (VP7 serotypes) among bovine rotavirus strains

“…Earlier works indicate the presence of at least two serotypes among group A bovine rotaviruses [2,3,9,15,21,26,32,35,36]. While the NCDV and UK strains have long been established as serotype 6 under the unified serotyping scheme, assignment of the bovine serotype 2 strains to a serotype under this scheme has just been taken into consideration.…”

Analyzing and monitoring of phage–bacteria interaction using CE

“…The pellet of the phage was suspended in 0.1% w/v ammonium acetate, then 20 mL 3% phosphotungstate was added, and finally it was placed on 400-mesh grid covered with carbon formvar (3.05 mm, Agar Scientific, UK). After 15 min, the grids were washed with deionized water and dried on a filter paper [25,26]. Micrographs of the phages were taken under 80 kV power by using a LEO 906 E electron microscope.…”